中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
1期
16-18
,共3页
黄陈%裘正军%江弢%刘俊%朱光辉%李海东
黃陳%裘正軍%江弢%劉俊%硃光輝%李海東
황진%구정군%강도%류준%주광휘%리해동
RNA干扰%信号传导与转录激活因子3%胰腺癌%血管生成
RNA榦擾%信號傳導與轉錄激活因子3%胰腺癌%血管生成
RNA간우%신호전도여전록격활인자3%이선암%혈관생성
RNA interference%Signal transducer and activator of transcription 3%Pancreatic carcinoma%Angiogenesis
目的 探讨RNA干扰(RNAi)抑制信号传导与转录激活因子3(STAT3)对人胰腺癌体外血管生成的影响及其机制.方法 RNAi抑制胰腺癌细胞株SW1990细胞中STAT3、噻唑蓝(MTT)和流式细胞技术分别检测胰腺癌细胞上清液对人脐静脉内皮细胞(HUVEC)细胞增殖和细胞周期的影响.体外迁移实验检测胰腺癌细胞上清液诱导的HUVEC迁移能力;酶联免疫吸附试验(ELISA)检测胰腺癌细胞上清液中血管内皮生长因子(VEGF)蛋白表达.结果 MTT和流式细胞仪结果显示RNAi抑制STAT3后,HUVEC增殖能力下降,24、48、72 h的细胞增殖率分别为(1.19±0.11)%、(1.62±0.15)%、(1.95±0.18)%;细胞周期阻滞于G0/G1期,为(80.95±7.49)%.体外迁移实验显示RNAi抑制STAT3后,HUVEC迁移能力明显减弱.ELISA显示RNAi抑制STAT3后,VEGF蛋白表达下降60%.结论 RNAi抑制STAT3可以通过下调VEGF,抑制胰腺癌细胞体外血管生成能力.
目的 探討RNA榦擾(RNAi)抑製信號傳導與轉錄激活因子3(STAT3)對人胰腺癌體外血管生成的影響及其機製.方法 RNAi抑製胰腺癌細胞株SW1990細胞中STAT3、噻唑藍(MTT)和流式細胞技術分彆檢測胰腺癌細胞上清液對人臍靜脈內皮細胞(HUVEC)細胞增殖和細胞週期的影響.體外遷移實驗檢測胰腺癌細胞上清液誘導的HUVEC遷移能力;酶聯免疫吸附試驗(ELISA)檢測胰腺癌細胞上清液中血管內皮生長因子(VEGF)蛋白錶達.結果 MTT和流式細胞儀結果顯示RNAi抑製STAT3後,HUVEC增殖能力下降,24、48、72 h的細胞增殖率分彆為(1.19±0.11)%、(1.62±0.15)%、(1.95±0.18)%;細胞週期阻滯于G0/G1期,為(80.95±7.49)%.體外遷移實驗顯示RNAi抑製STAT3後,HUVEC遷移能力明顯減弱.ELISA顯示RNAi抑製STAT3後,VEGF蛋白錶達下降60%.結論 RNAi抑製STAT3可以通過下調VEGF,抑製胰腺癌細胞體外血管生成能力.
목적 탐토RNA간우(RNAi)억제신호전도여전록격활인자3(STAT3)대인이선암체외혈관생성적영향급기궤제.방법 RNAi억제이선암세포주SW1990세포중STAT3、새서람(MTT)화류식세포기술분별검측이선암세포상청액대인제정맥내피세포(HUVEC)세포증식화세포주기적영향.체외천이실험검측이선암세포상청액유도적HUVEC천이능력;매련면역흡부시험(ELISA)검측이선암세포상청액중혈관내피생장인자(VEGF)단백표체.결과 MTT화류식세포의결과현시RNAi억제STAT3후,HUVEC증식능력하강,24、48、72 h적세포증식솔분별위(1.19±0.11)%、(1.62±0.15)%、(1.95±0.18)%;세포주기조체우G0/G1기,위(80.95±7.49)%.체외천이실험현시RNAi억제STAT3후,HUVEC천이능력명현감약.ELISA현시RNAi억제STAT3후,VEGF단백표체하강60%.결론 RNAi억제STAT3가이통과하조VEGF,억제이선암세포체외혈관생성능력.
Objective To investigate the effect and mechanism of RNA interference ( RNAi)-mediated signal transducer and activator of transcription 3 ( STAT3 ) inhibition on human pancreatic cancer cells angiogenesis in vitro.Methods RNAi was used to inhibit STAT3 gene of SW1990 cells.Methyl thiazol tetrazolium (MTT) assay and flow cytometric analysis were performed to detect cell proliferation and cell cycle of human umbilical vein endothelial cell ( HUVEC),respectively.The migration ability of HUVECs was determined by cell migration assay.Enzyme linked immunosorbent assay (ELISA) was used to detect the protein expression of the vascular endothelial growth factor (VEGF).Results Inhibition of STAT3 with RNAi significantly inhibited the proliferation of HUVECs and decreased the migration ability of HUVECs invitro.The cell growth rate at 24,48,72 h in SW1990-RNAi group was (1.19 ±0.11)%,( 1.62 ± 0.15 ) %,( 1.95 ± 0.18 ) %,respectively.The percentage of HUVECs at G0/G1 phase in SW1990-RNAi group was (80.95 ± 7.49)%.Moreover,the VEGF protein expression in SW1990-RNAi cells was reduced by 60%.Conclusion Inhibition of STAT3 with RNAi can significantly inhibit the angiogenesis ability of pancreatic cancer cells through down-regulating VEGF,which may provide a novel strategy in preventing the angiogenesis of pancreatic cancer.