中华检验医学杂志
中華檢驗醫學雜誌
중화검험의학잡지
CHINESE JOURNAL OF LABORATORY MEDICINE
2012年
1期
47-52
,共6页
傅修涛%代智%赵一鸣%周正君%周俭%樊嘉
傅脩濤%代智%趙一鳴%週正君%週儉%樊嘉
부수도%대지%조일명%주정군%주검%번가
癌,肝细胞%肝肿瘤%抗原,分化,B淋巴细胞%组织相容性抗原Ⅱ类%预后
癌,肝細胞%肝腫瘤%抗原,分化,B淋巴細胞%組織相容性抗原Ⅱ類%預後
암,간세포%간종류%항원,분화,B림파세포%조직상용성항원Ⅱ류%예후
Carcinoma,hepatocellular%Liver neoplasms%Antigens,differentiation,B-lymphocyte%Histocompatibility antigens class Ⅱ%Prognosis
目的 研究分析白细胞分化抗原74(duster of differentiation 74,CD74)分子在肝癌组织及肝癌细胞系中的表达水平与肝癌预后的关系.方法 用免疫印迹法( Western blot,WB)检测高转移潜能肝癌细胞系( MHCC-LM3、MHCC-97H)、低转移潜能肝癌细胞系(MHCC-97L)及无转移潜能肝癌细胞系(Hep-G2)中CD74的表达；将320例行根治性肝癌切除术患者的肝癌及癌旁组织与5份正常移植供体肝的石蜡包埋标本做成高密度组织芯片,对其用免疫组织化学法检测癌内、癌旁及正常肝组织中CD74的表达水平.Kaplan-Meier法绘制生存曲线,Logrank法比较组间生存差异,并用Cox比例风险回归模型进行预后影响因素的单因素及多因素分析.结果 肝癌细胞系( MHCC-LM3、MHCC-97H、MHCC-97L、Hep-G2)中CD74的平均相对表达量分别为0.622±0.078、0.732±0.083、1.224±0.014、1.374±0.006,高转移潜能肝癌细胞系(MHCC-LM3、MHCC-97H)与低转移潜能肝癌细胞系(MHCC-97L)及无转移潜能肝癌细胞系(Hep-G2)各指标间及各组间CD74表达差异均有统计学意义(t值分别为- 13.308、- 16.849、- 10.177、- 13.436、- 17.057,P均＜0.01),CD74的表达量随肝癌细胞系转移潜能的递减而递增；320份肝癌患者标本中,CD74在癌内组织中阳性表达221份,阴性表达99份,癌旁组织中仅微量表达,5份正常肝组织中均不表达；肝癌患者癌内CD74的表达在不同性别组间差异有统计学意义(x2=3.954,P＜0.05),而在按年龄、乙型肝炎表面抗原、肝硬化、甲胎蛋白、肿瘤数目与大小、肿瘤包膜、血管侵犯、Edmondson分级及肿瘤、结节、转移分类(tumor、nodes、metastasis classification,TNM)分期等临床病理特征分组的分布差异均无统计学意义(x2值分别为0.053、0.141、1.200、0.000、0.277、1.975、0.263、1.044、0.000、0.433,P均＞0.05)；Kaplan-Meier生存曲线及Logrank分析显示癌内CD74表达阳性者预后较好(x2=5.620,P＜0.05)；Cox模型多因素分析显示CD74是独立的预后影响因素[风险比(hazard ratio,HR)=0.721,95％可信限(confidence interval,CI) =0.522 ～0.996,P＜0.05].结论 CD74可作为根治性肝癌切除术后病理判断患者预后的指标,其机制可能与引发肝癌细胞凋亡相关.
目的 研究分析白細胞分化抗原74(duster of differentiation 74,CD74)分子在肝癌組織及肝癌細胞繫中的錶達水平與肝癌預後的關繫.方法 用免疫印跡法( Western blot,WB)檢測高轉移潛能肝癌細胞繫( MHCC-LM3、MHCC-97H)、低轉移潛能肝癌細胞繫(MHCC-97L)及無轉移潛能肝癌細胞繫(Hep-G2)中CD74的錶達；將320例行根治性肝癌切除術患者的肝癌及癌徬組織與5份正常移植供體肝的石蠟包埋標本做成高密度組織芯片,對其用免疫組織化學法檢測癌內、癌徬及正常肝組織中CD74的錶達水平.Kaplan-Meier法繪製生存麯線,Logrank法比較組間生存差異,併用Cox比例風險迴歸模型進行預後影響因素的單因素及多因素分析.結果 肝癌細胞繫( MHCC-LM3、MHCC-97H、MHCC-97L、Hep-G2)中CD74的平均相對錶達量分彆為0.622±0.078、0.732±0.083、1.224±0.014、1.374±0.006,高轉移潛能肝癌細胞繫(MHCC-LM3、MHCC-97H)與低轉移潛能肝癌細胞繫(MHCC-97L)及無轉移潛能肝癌細胞繫(Hep-G2)各指標間及各組間CD74錶達差異均有統計學意義(t值分彆為- 13.308、- 16.849、- 10.177、- 13.436、- 17.057,P均＜0.01),CD74的錶達量隨肝癌細胞繫轉移潛能的遞減而遞增；320份肝癌患者標本中,CD74在癌內組織中暘性錶達221份,陰性錶達99份,癌徬組織中僅微量錶達,5份正常肝組織中均不錶達；肝癌患者癌內CD74的錶達在不同性彆組間差異有統計學意義(x2=3.954,P＜0.05),而在按年齡、乙型肝炎錶麵抗原、肝硬化、甲胎蛋白、腫瘤數目與大小、腫瘤包膜、血管侵犯、Edmondson分級及腫瘤、結節、轉移分類(tumor、nodes、metastasis classification,TNM)分期等臨床病理特徵分組的分佈差異均無統計學意義(x2值分彆為0.053、0.141、1.200、0.000、0.277、1.975、0.263、1.044、0.000、0.433,P均＞0.05)；Kaplan-Meier生存麯線及Logrank分析顯示癌內CD74錶達暘性者預後較好(x2=5.620,P＜0.05)；Cox模型多因素分析顯示CD74是獨立的預後影響因素[風險比(hazard ratio,HR)=0.721,95％可信限(confidence interval,CI) =0.522 ～0.996,P＜0.05].結論 CD74可作為根治性肝癌切除術後病理判斷患者預後的指標,其機製可能與引髮肝癌細胞凋亡相關.
목적 연구분석백세포분화항원74(duster of differentiation 74,CD74)분자재간암조직급간암세포계중적표체수평여간암예후적관계.방법 용면역인적법( Western blot,WB)검측고전이잠능간암세포계( MHCC-LM3、MHCC-97H)、저전이잠능간암세포계(MHCC-97L)급무전이잠능간암세포계(Hep-G2)중CD74적표체；장320례행근치성간암절제술환자적간암급암방조직여5빈정상이식공체간적석사포매표본주성고밀도조직심편,대기용면역조직화학법검측암내、암방급정상간조직중CD74적표체수평.Kaplan-Meier법회제생존곡선,Logrank법비교조간생존차이,병용Cox비례풍험회귀모형진행예후영향인소적단인소급다인소분석.결과 간암세포계( MHCC-LM3、MHCC-97H、MHCC-97L、Hep-G2)중CD74적평균상대표체량분별위0.622±0.078、0.732±0.083、1.224±0.014、1.374±0.006,고전이잠능간암세포계(MHCC-LM3、MHCC-97H)여저전이잠능간암세포계(MHCC-97L)급무전이잠능간암세포계(Hep-G2)각지표간급각조간CD74표체차이균유통계학의의(t치분별위- 13.308、- 16.849、- 10.177、- 13.436、- 17.057,P균＜0.01),CD74적표체량수간암세포계전이잠능적체감이체증；320빈간암환자표본중,CD74재암내조직중양성표체221빈,음성표체99빈,암방조직중부미량표체,5빈정상간조직중균불표체；간암환자암내CD74적표체재불동성별조간차이유통계학의의(x2=3.954,P＜0.05),이재안년령、을형간염표면항원、간경화、갑태단백、종류수목여대소、종류포막、혈관침범、Edmondson분급급종류、결절、전이분류(tumor、nodes、metastasis classification,TNM)분기등림상병리특정분조적분포차이균무통계학의의(x2치분별위0.053、0.141、1.200、0.000、0.277、1.975、0.263、1.044、0.000、0.433,P균＞0.05)；Kaplan-Meier생존곡선급Logrank분석현시암내CD74표체양성자예후교호(x2=5.620,P＜0.05)；Cox모형다인소분석현시CD74시독립적예후영향인소[풍험비(hazard ratio,HR)=0.721,95％가신한(confidence interval,CI) =0.522 ～0.996,P＜0.05].결론 CD74가작위근치성간암절제술후병리판단환자예후적지표,기궤제가능여인발간암세포조망상관.
Objective By analyzing the expression of cluster of differentiation 74 (CD74) in hepatocellular carcinoma (HCC) and HCC cell lines,the correlation between the level of CD74 expression and the patients' prognosis was investigated.Methods The expression of CD74 in high metastatic potential HCC cell lines(MHCC-LM3,MHCC-97H),low metastatic potential HCC cell line( MHCC-97L),and no metastatic potential HCC cell line(Hep-G2) were estimated by Western blot.The paraffin embedded tissues which include intra-tumor and paratumor tissues were collected from 320 patients who had received HCC curative surgical resection and 5 normal liver tissues from the donors of liver tranplantation.The high density tissue micro-array was made of these specimens. Immunol-histochemistry was applied to discover the different levels of CD74 in tumor,paratumor and normal liver tissues.Survival curves were generated by the Kaplan-Meier method and verified by the Logrank test.Cox proportional hazards regression analysis was applied to estimate the prognostic factors in multivariate analysis.Results The expression level of CD74 was significantly higher in low metastatic potential and no metastatic potential HCC cell lines (MHCC-97L 1.224 ±0.014,Hep-G2 1.374 ±0.006) than that in high metastatic potential ones( MHCC-LM3 0.622 ±0.078,MHCC-97H 0.732 ± 0.083 ).Significant differences can be found between the groups (t =- 13.308,- 16.849,- 10.177,- 13.436,- 17.057; P ＜0.01 ).Meanwhile,in tumor tissues,the CD74 was expressed positively in 221 patients and negatively in 99 patients.But CD74 was expressed slightly in paratumor and negatively in 5 normal liver tissues.There's no significant differences between the groups categorization according to age,HBsAg,cirrhosis,AFP level,tumor number,tumor size,tumor capsule,blood vessel invasion,Edmondson Grades and tumor nodes metastasis classification (TNM) stages (x2 =0.053,0.141,1.200,0.000,0.277,1.975,0.263,1.044,0.000,0.433 ; P ＞ 0.05 ),except gender (x2 =3.954,P ＜ 0.05).Kaplan-Meier method showed that patients with positively expression of CD74 had better prognosis than others (x2 =5.620,P ＜ 0.05 ).Cox proportional hazards regression analysis showed that CD74 was a significant and independent prognostic factor of survival [ hazard ratio (HR) =0.721,95％confidence interval (CI) =0.522 - 0.996,P ＜ 0.05 ].Conclusion The expression of CD74 in hepatocellular carcinoma could be a biomarker of the prognosis and there's some potential correlation with cancer cell apoptosis.
