CAJ | 학술논문

通过构建香猪肌肉组织cDNA文库,并在文库中随机挑选克隆进行测序的方法,获得了131个香猪肌肉EST序列.在这131个EST序列所代表的109个单一克隆中,有99个为人类及其他物种的同源序列,3个为已知的猪的ESTs,7个为未知ESTs.对这10个已知、未知ESTs进行开放阅读框预测并进行B1ast分析,没有找到高度同源的氨基酸序列.对上述EST所对应的基因功能分析结果表明,除去27.27%的EST未能分类外,克隆到的EST大多来自与基因/蛋白的表达调控相关的基因(占45.46%).来自具有其他功能的基因的EST依次是细胞代谢占10.10%、细胞结构/迁移占10.10%、细胞/机体防御占5.05%和细胞信号/传导占2.02%.没有发现和细胞分裂相关的已知功能基因.本研究结果为中国地方品种香猪提供了第一个骨骼肌的基因表达谱,为今后寻找猪肌肉生长和肉用品质的候选基因奠定了基础.
통과구건향저기육조직cDNA문고,병재문고중수궤도선극륭진행측서적방법,획득료131개향저기육EST서렬.재저131개EST서렬소대표적109개단일극륭중,유99개위인류급기타물충적동원서렬,3개위이지적저적ESTs,7개위미지ESTs.대저10개이지、미지ESTs진행개방열독광예측병진행B1ast분석,몰유조도고도동원적안기산서렬.대상술EST소대응적기인공능분석결과표명,제거27.27%적EST미능분류외,극륭도적EST대다래자여기인/단백적표체조공상관적기인(점45.46%).래자구유기타공능적기인적EST의차시세포대사점10.10%、세포결구/천이점10.10%、세포/궤체방어점5.05%화세포신호/전도점2.02%.몰유발현화세포분렬상관적이지공능기인.본연구결과위중국지방품충향저제공료제일개골격기적기인표체보,위금후심조저기육생장화육용품질적후선기인전정료기출.
A Longissimus Dorsi muscle cDNA library of Xiang Pig was constructed, and 131 randomly isolated clones were sequenced in this study. The results of bioinformatics analysis showed that 131 ESTs represented 109 unique clones sequences, of which 99 showed homology to previously identified genes in humans or other mammals, 3 matched other uncharacterized expressed sequence tags (ESTs), and 7 showed no significant matches to sequences already present in DNA databases. No protein matches were found for 10 ESTs. Functional analysis of the ESTs showed that a considerable proportion of them encoded proteins involved in gene/protein expression (45.46%). Other classes included genes involved in metabolism (10.10%), cell structure/motility (10.10%), cell/organism defense (5.05%), cell signaling/communication (2.02%), and cell division (0.0%).Unclassified genes constituted the remaining 27.27%. This study reported the results of the first gene expression profile analysis of Chinese native Xiang Pig skeletal muscle cells, thereby greatly facilitating the functional study of candidate genes involved in muscle growth as well as in the improvement of meat quality in domestic pigs.