重庆医科大学学报
重慶醫科大學學報
중경의과대학학보
UNIVERSITATIS SCIENTIAE MEDICINAE CHONGQING
2009年
10期
1351-1354
,共4页
青蒿琥酯钠%瘢痕疙瘩%成纤维细胞%分化抑制因子1(Id1)
青蒿琥酯鈉%瘢痕疙瘩%成纖維細胞%分化抑製因子1(Id1)
청호호지납%반흔흘탑%성섬유세포%분화억제인자1(Id1)
Artesunate%Keloid%Fibroblast%Inhibitor of differentiation 1 (Id1)
目的:探讨青蒿琥酯对瘢痕疙瘩(Keloid)成纤维细胞(Fibroblast,fb)的增殖抑制作用及其作用机制.方法:将60mg/L的青蒿琥酯作用于体外培养的瘢痕疙瘩成纤维细胞24 h,MTT法检测细胞生长抑制率,免疫细胞化学和western blotting法检测分化抑制因子1(Inhibitor of differentiation 1,Id1)的表达情况.结果:在青蒿琥酯的作用下,成纤维细胞的Id1表达降低.结论:青蒿琥酯抑制成纤维细胞的机制可能与抑制Id1的表达有关.
目的:探討青蒿琥酯對瘢痕疙瘩(Keloid)成纖維細胞(Fibroblast,fb)的增殖抑製作用及其作用機製.方法:將60mg/L的青蒿琥酯作用于體外培養的瘢痕疙瘩成纖維細胞24 h,MTT法檢測細胞生長抑製率,免疫細胞化學和western blotting法檢測分化抑製因子1(Inhibitor of differentiation 1,Id1)的錶達情況.結果:在青蒿琥酯的作用下,成纖維細胞的Id1錶達降低.結論:青蒿琥酯抑製成纖維細胞的機製可能與抑製Id1的錶達有關.
목적:탐토청호호지대반흔흘탑(Keloid)성섬유세포(Fibroblast,fb)적증식억제작용급기작용궤제.방법:장60mg/L적청호호지작용우체외배양적반흔흘탑성섬유세포24 h,MTT법검측세포생장억제솔,면역세포화학화western blotting법검측분화억제인자1(Inhibitor of differentiation 1,Id1)적표체정황.결과:재청호호지적작용하,성섬유세포적Id1표체강저.결론:청호호지억제성섬유세포적궤제가능여억제Id1적표체유관.
Objective: To investigate the inhibition of artesunate on fibroblasts in vitro derived from human keloid. Methods:60mg/L artesunate was added to the medium.The effects of artesunate on fibroblasts in vitro were observed after 24h.The inhibitory rate of the cellular growth was meassured using MTT assay .The expression of Id 1 protein was detected by immunohistochemistry and western blotting. Results:The data indicated that the expression of Id1 of fibroblasts derived from keloid were depressed by artesunat. Conclusion:The mechanism of inhibit fibroblast proliferation by artesunat might be related to the depression of protein expression of Id1.
