CAJ | 학술논문

采用紫外光固定化法,对组织培养用聚苯乙烯板进行半乳糖糖化温敏修饰.通过红外光谱(ATR-FTIR)和X射线光电子能谱(XPS)对改性表面的化学组成及结构进行了表征,并采用原子力显微镜(AFM)观察了改性表面形貌,发现改性表面比未经修饰表面粗糙度增加.静态接触角测试结果表明,改性表面具有良好的温度响应性.对人肝肿瘤(HepG-2)细胞在改性表面的吸/脱附行为的研究结果表明,HepG-2细胞在半乳糖糖化温敏表面表现出比在未经修饰聚苯乙烯细胞培养板表面更好的生长趋势,当环境温度降低时,细胞发生自动脱附,避免了酶解法对细胞功能造成的损伤.
채용자외광고정화법,대조직배양용취분을희판진행반유당당화온민수식.통과홍외광보(ATR-FTIR)화X사선광전자능보(XPS)대개성표면적화학조성급결구진행료표정,병채용원자력현미경(AFM)관찰료개성표면형모,발현개성표면비미경수식표면조조도증가.정태접촉각측시결과표명,개성표면구유량호적온도향응성.대인간종류(HepG-2)세포재개성표면적흡/탈부행위적연구결과표명,HepG-2세포재반유당당화온민표면표현출비재미경수식취분을희세포배양판표면경호적생장추세,당배경온도강저시,세포발생자동탈부,피면료매해법대세포공능조성적손상.
Poly(N-isopropylacrylamide) and acrylic acid were grafted onto tissue culture polystyrene(TCPS)by UV irradiation, followed by immobilization of the monoamine terminated lactobionie (L-NH_2 ) to acrylic acid carboxyls. The compositions of modified surfaces were characterized by ATR-FTIR and XPS. The morphologies were observed via AFM. Results from drop contact angle measurement indicate that the modified surfaces show hydrophobic/hydrophilic property alterations in response to temperature. Adhesion and detachment of hepatic tumor cells(HepG-2) were examined on virgin surface and modified surfaces. Immobilization of L-NH_2 facilitated HepG-2 cells adhesion on saccharified thermoresponsive surface. Moreover, HepG-2 cells cultured on modified surfaces can be recovered non-invasively by decreasing temperature.