CAJ | 학술논문

目的:通过观察神经特异性Nbn基因敲除(Nbn-CNS-del)和对照(Nbn-CNS-Ctr)小鼠小脑神经元发育的形态学变化,探讨DNA损伤修复基因Nbn在小鼠小脑发育过程中的作用.方法:应用免疫组织化学技术和体视学方法对生后(P) 7、 14、 21d的Nbn-CNS-del和Nbn-CNS-Ctr小鼠小脑进行系统观察和定量分析.结果:与Nbn-CNS-ctr小鼠相比,Nbn-CNS-del小鼠生后各时间相点小脑发育迟缓,皮质各层界限不清,细胞排列紊乱,至21d未形成正常小脑皮质的3层结构;NeuN阳性表达的颗粒细胞面数密度和体密度值明显减小;D-28K阳性表达的浦肯野细胞面数密度和体密度值增加.结论:DNA损伤修复基因Nbn可能是小脑发育过程中的重要基因,影响小鼠小脑神经元的发育和成熟.
목적:통과관찰신경특이성Nbn기인고제(Nbn-CNS-del)화대조(Nbn-CNS-Ctr)소서소뇌신경원발육적형태학변화,탐토DNA손상수복기인Nbn재소서소뇌발육과정중적작용.방법:응용면역조직화학기술화체시학방법대생후(P) 7、 14、 21d적Nbn-CNS-del화Nbn-CNS-Ctr소서소뇌진행계통관찰화정량분석.결과:여Nbn-CNS-ctr소서상비,Nbn-CNS-del소서생후각시간상점소뇌발육지완,피질각층계한불청,세포배렬문란,지21d미형성정상소뇌피질적3층결구;NeuN양성표체적과립세포면수밀도화체밀도치명현감소;D-28K양성표체적포긍야세포면수밀도화체밀도치증가.결론:DNA손상수복기인Nbn가능시소뇌발육과정중적중요기인,영향소서소뇌신경원적발육화성숙.
Objective: To investigate the role of DNA repair gene Nbn on the cerebellar development by observing the morphological changes of Nbn-CNS-del and Nbn-CNS-Ctr mouse cerebellar neurons. Methods: Immunohistochemistry and stereological methods were used to analyse the cerebellar development in the mice at postnatal day 7, 14 and 21. Results: Compared with the Nbn-CNS-Ctr mouse, the cerebellar development in Nbn-CNS-del mouse was delayed, and a normal three-layer structure was not observed at postnatal day 21. The profile numberical densities and volume density of NeuN positive granule cells were decreased, while those of D-28K positive Purkinje cells were increased. Conclusion: DNA repair gene Nbn might be a key gene during mouse cerebellar development, impacting the development and maturity of cerebellar neurons of mice.