CAJ | 학술논문

核糖体蛋白S5是核糖体蛋白家族的重要成员,在核糖体中发挥重要作用.利用本实验室获得的部分序列设计引物,采用3'-RACE技术从巴西橡胶树胶乳中获得一个核糖体蛋白S5基因的完整阅读框,该基因编码209个氨基酸的多肽,含有一个典型的真核生物核糖体蛋白S5结构域.在HbRPS5氨基酸序列中没有预测到跨膜区和信号肽,二级结构分析表明HbRPS5属于混合型蛋白.对14个RPS5系统进化分析表明,巴西橡胶树的RPS5基因在进化上具有保守性,与植物类拟南芥的RPS5基因亲缘关系最近,而与人和酵母等亲缘关系相对较远.该基因的克隆和生物信息学分析为深入研究其功能奠定了基础.
핵당체단백S5시핵당체단백가족적중요성원,재핵당체중발휘중요작용.이용본실험실획득적부분서렬설계인물,채용3'-RACE기술종파서상효수효유중획득일개핵당체단백S5기인적완정열독광,해기인편마209개안기산적다태,함유일개전형적진핵생물핵당체단백S5결구역.재HbRPS5안기산서렬중몰유예측도과막구화신호태,이급결구분석표명HbRPS5속우혼합형단백.대14개RPS5계통진화분석표명,파서상효수적RPS5기인재진화상구유보수성,여식물류의남개적RPS5기인친연관계최근,이여인화효모등친연관계상대교원.해기인적극륭화생물신식학분석위심입연구기공능전정료기출.
The ribosomal protein S5 is the key member of ribosomal protein family,and it plays an important role in the ribosomal proteins.The primers designed based on the known sequences,the gene was cloned with 3'-RACE technique from Hevea brasiliensis latex.The open reading frame encoded 209 amino acids,containing a conserved ribosomal S5 domain,so the gene was named as HbRRS5.No signal peptide and transmembrane structure were predicted within HbRPS5.The result of secondary structure predicted showed that the protein was classified as mixed type.The result of phylogenetic tree indicated that HbRPS5 displayed a high degree of sequence similarity to AtRPS5,but not to animals,such as Homo sapiens,Rattus norveegicus,and 80 on.This paper will be benefit to further analyze the functions of HbRPS5.