CAJ | 학술논문

一些对蛋白质活性很重要的残基在进化过程中是高度保守的,另有一些残基通过共进化来维持蛋白质结构和功能上的稳定.由于共进化残基分析可在未知蛋白质结构时,仅依据序列推断残基间的相互作用,因此在蛋白质结构和功能预测上具有重要的研究意义.当前分析共进化残基的方法主要有基于相关系数的方法、基于微扰理论的方法、参数检验法等.然而,由于存在蛋白质系统进化的背景干扰,目前共进化残基分析的精度仍有待进一步提高.本文概述了蛋白质共进化分析的方法及其研究进展,并对其发展趋势进行了预测.
일사대단백질활성흔중요적잔기재진화과정중시고도보수적,령유일사잔기통과공진화래유지단백질결구화공능상적은정.유우공진화잔기분석가재미지단백질결구시,부의거서렬추단잔기간적상호작용,인차재단백질결구화공능예측상구유중요적연구의의.당전분석공진화잔기적방법주요유기우상관계수적방법、기우미우이론적방법、삼수검험법등.연이,유우존재단백질계통진화적배경간우,목전공진화잔기분석적정도잉유대진일보제고.본문개술료단백질공진화분석적방법급기연구진전,병대기발전추세진행료예측.
Some functionally important protein residues are highly conserved,while co-evolving residues are also able to preserve structure and function of proteins. Because protein coevolution analysis can predict residues-residues interactions from their sequences without structural information,it is very significant in protein structure and function prediction. Nowadays,protein coevolution analysis is mainly based on coefficient correlation methods,perturbation methods and parameter test. However,because of phylogenetic noise,the accuracy of protein coevolution analysis needs to be improved. Here we briefly introduce methods and advances of protein coevolution analysis and point out its research directions.