CAJ | 학술논문

藻类是水生生态系统中的敏感物种.在环境生物学研究中,基因转录水平通常作为衡量基因表达水平变化的指标.研究建立了定量PCR技术(Real-time PCR)检测小球藻基因转录变化的研究体系.以3个主要光合作用基因(psaB、psbC、rbcL)为靶标基因,研究了除草剂草丁瞵(Glufosinate)、阿特拉津(Atrazine)和禾草灵(Diclofop-methyl)对基因转录的影响,结果表明3种供试药物对小球藻光合作用基因psbC和rbcL相对表达量有显著地抑制作用.这表明Real-time PCR可以作为评定水生环境中异源化合物毒性的有效方法.
조류시수생생태계통중적민감물충.재배경생물학연구중,기인전록수평통상작위형량기인표체수평변화적지표.연구건립료정량PCR기술(Real-time PCR)검측소구조기인전록변화적연구체계.이3개주요광합작용기인(psaB、psbC、rbcL)위파표기인,연구료제초제초정린(Glufosinate)、아특랍진(Atrazine)화화초령(Diclofop-methyl)대기인전록적영향,결과표명3충공시약물대소구조광합작용기인psbC화rbcL상대표체량유현저지억제작용.저표명Real-time PCR가이작위평정수생배경중이원화합물독성적유효방법.
Among the aquatic organisms, algae are one of the most susceptible organisms to pollution in water. Transcriptional abundance has been gradually used as a surrogate for gene expression in environmental biology. In the present study, real-time PCR assay was established to quantify the transcript abundances of three photosystem genes (psaB,psbC and rbcL) in Chlorella vulgaris, and the acute toxicity of three herbicides (glufosiuate, atrazine and diclofop-methyl) was assessed using this method. The results demonstrated that real-time PCR is an efficient technique to assess the toxicity of xenobiotic compounds in aquatic system.