中华病理学杂志
中華病理學雜誌
중화병이학잡지
Chinese Journal of Pathology
2011年
3期
177-181
,共5页
结节病%颗粒物%光谱分析%拉曼
結節病%顆粒物%光譜分析%拉曼
결절병%과립물%광보분석%랍만
Sarcoidosis%Particulate matter%Spectrum analysis,Raman
目的 分析结节病肉芽肿细胞(吞噬细胞、类上皮细胞、多核巨细胞)内空气细颗粒物(PM2.5)的元素组成及来源,探讨结节病与PM2.5的相关性.方法 收集50例结节病病变组织、10例非结节病成人尸检肺组织、18例PM2.5支气管灌注染毒大鼠肺组织(有肉芽肿病灶)石蜡包埋标本,采集大气中PM2.5,分别行HE染色、沃森-斯塔理(Warthin-Starry,WS)银染色,应用共聚焦显微镜拉曼光谱和透射电镜-能谱对上述部分病例标本肉芽肿细胞和尘细胞内的PM2.5及大气PM2.5的成分进行分析,用X线荧光分析技术对所采集大气PM2.5的主要元素成分进行分析.结果 结节病病变组织、非结节病成人尸检肺组织、PM2.5支气管灌注染毒大鼠肺组织及大气中PM2.5共4组标本中PM2.5的拉曼光谱和元素组成非常相似,均以碳质成分为主要特征.结论 为结节病肉芽肿细胞中的PM2.5来源于大气PM2.5提供了进一步的依据,从而不能完全除外结节病患者可能是对PM2.5易感的个体的可能性.
目的 分析結節病肉芽腫細胞(吞噬細胞、類上皮細胞、多覈巨細胞)內空氣細顆粒物(PM2.5)的元素組成及來源,探討結節病與PM2.5的相關性.方法 收集50例結節病病變組織、10例非結節病成人尸檢肺組織、18例PM2.5支氣管灌註染毒大鼠肺組織(有肉芽腫病竈)石蠟包埋標本,採集大氣中PM2.5,分彆行HE染色、沃森-斯塔理(Warthin-Starry,WS)銀染色,應用共聚焦顯微鏡拉曼光譜和透射電鏡-能譜對上述部分病例標本肉芽腫細胞和塵細胞內的PM2.5及大氣PM2.5的成分進行分析,用X線熒光分析技術對所採集大氣PM2.5的主要元素成分進行分析.結果 結節病病變組織、非結節病成人尸檢肺組織、PM2.5支氣管灌註染毒大鼠肺組織及大氣中PM2.5共4組標本中PM2.5的拉曼光譜和元素組成非常相似,均以碳質成分為主要特徵.結論 為結節病肉芽腫細胞中的PM2.5來源于大氣PM2.5提供瞭進一步的依據,從而不能完全除外結節病患者可能是對PM2.5易感的箇體的可能性.
목적 분석결절병육아종세포(탄서세포、류상피세포、다핵거세포)내공기세과립물(PM2.5)적원소조성급래원,탐토결절병여PM2.5적상관성.방법 수집50례결절병병변조직、10례비결절병성인시검폐조직、18례PM2.5지기관관주염독대서폐조직(유육아종병조)석사포매표본,채집대기중PM2.5,분별행HE염색、옥삼-사탑리(Warthin-Starry,WS)은염색,응용공취초현미경랍만광보화투사전경-능보대상술부분병례표본육아종세포화진세포내적PM2.5급대기PM2.5적성분진행분석,용X선형광분석기술대소채집대기PM2.5적주요원소성분진행분석.결과 결절병병변조직、비결절병성인시검폐조직、PM2.5지기관관주염독대서폐조직급대기중PM2.5공4조표본중PM2.5적랍만광보화원소조성비상상사,균이탄질성분위주요특정.결론 위결절병육아종세포중적PM2.5래원우대기PM2.5제공료진일보적의거,종이불능완전제외결절병환자가능시대PM2.5역감적개체적가능성.
Objective To explore the source of the fine particulate matter (PM2.5) in the sarcoidosis granulomatous cell and the relationship between the sarcoidosis and the PM2.5 in the atmosphere.Methods Paraffin-embedded tissues of 50 cases of human sarcoidosis biopsy samples, 10 cases of nonsarcoidosis autopsy lung samples, 18 cases of lung tissues (with granulomatous lesions) of rats exposed to PM2.5 by bronchial infusion, and the free PM2.5 sample in the atmosphere were collected. The characteristics of tissues above mentioned were observed under the light microscopy, which stained by HE staining and Warthin-Starry silver staining. The characteristics of the PM2.5 in the four groups were analyzed using confocal Raman microscopy. The component of the PM2.5 in the sarcoidosis granuloma was analyzed using transmission electron microscope-energy dispersive X-ray detector (TEM-EDX), and the component of the PM2.5 in the atmosphere was analyzed with X-ray fluorescence separately. Results The PM2.5 in the four groups have the similar Raman spectrum, they share the feature of carbonaceous composition, the element component of PM2.5 in the human sarcoidosis was the same as PM2.5 in the atmosphere. Conclusion The study provided the further evidence that the PM2.5 in the sarcoidosis lesion was from PM2.5 in the atmosphere,and it should be not excepted that sarcoidosis may be a sensitive individual reaction to the PM2.5 inhaled from the atmosphere.
