CAJ | 학술논문

目的观察缓激肽(BK)对人肺癌细胞株A549迁移、侵袭能力的影响.方法应用划痕愈合实验和Transwell实验检测BK对40例人肺癌细胞株A549的划痕愈合和运动侵袭能力.通过Western blot分析刺激和抑制缓激肽各20例后基质金属蛋白酶(MMP)-2、MMP-9和E-钙黏素(E-Cadherin)的变化并研究其机制.结果划痕实验后分别测量愈合距离,结果示BK组24h和48h后迁移细胞数分别为69.2±3.3、94.1±2.9,而阻断其受体或者ERK1/2通路则迁移细胞数分别为51.2±2.1、73.2±2.7和47.5±3.4、77.6±3.8.Transwell实验检测BK对A549侵袭能力的影响结果显示BK可以明显促进A549的侵袭能力.阻断BK受体或ERK1/2通路则可以抑制其侵袭能力.结论缓激肽促进人肺腺癌细胞株A549的迁移和侵袭能力.
목적 관찰완격태(BK)대인폐암세포주A549천이、침습능력적영향.방법 응용화흔유합실험화Transwell실험검측BK대40례인폐암세포주A549적화흔유합화운동침습능력.통과Western blot분석자격화억제완격태각20례후기질금속단백매(MMP)-2、MMP-9화E-개점소(E-Cadherin)적변화병연구기궤제.결과 화흔실험후분별측량유합거리,결과 시BK조24h화48h후천이세포수분별위69.2±3.3、94.1±2.9,이조단기수체혹자ERK1/2통로칙천이세포수분별위51.2±2.1、73.2±2.7화47.5±3.4、77.6±3.8.Transwell실험검측BK대A549침습능력적영향결과 현시BK가이명현촉진A549적침습능력.조단BK수체혹ERK1/2통로칙가이억제기침습능력.결론 완격태촉진인폐선암세포주A549적천이화침습능력.
Objective To investigate the effect of bradykinin (BK) on human lung cancer cell line A549 migration,invasion ability. Methods Scratch Healing and Transwell assay of bradykinin were used to detect scratch healing and movement on A549 human lung cancer cells. Analysis the changes of matrix metalloproteinase (MMP)-2, MMP-9 and E-Cadherin after stimulate and suppress 20 cases respectively in the aim to describe the mechanism by Western blotting. Results In the former, BK group promote cell migration significantly,migrated cell was 69.2±3.3, 94.1±2.9 respectively after 24 h and 48 h,blocking its receptor or ERK1/2 pathway inhibit migration,migrated cell was 51.2±2.1, 73.2±2.7 and 47.5±3.4,77.6±3.8 after 24 h and 48 h. In the latter,Transwell assay of A549 BK showed invasion of A549 BK can significantly promote the invasion ability of A549 to penetrate the basement membrane of the cell. And blocking BK receptor or ERK1/2 pathway can inhibit the invasion. Conclusion Bradykinin promote human lung adenocarcinoma cell line A549 migration and invasion.