中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2011年
3期
265-268
,共4页
刘道洁%董培玲%丁惠国%赵敬敬%李娟%孟庆华%闫丽%孙斌%武彦宁%王延军
劉道潔%董培玲%丁惠國%趙敬敬%李娟%孟慶華%閆麗%孫斌%武彥寧%王延軍
류도길%동배령%정혜국%조경경%리연%맹경화%염려%손빈%무언저%왕연군
记忆性T细胞%慢性乙型肝炎%IFN-α
記憶性T細胞%慢性乙型肝炎%IFN-α
기억성T세포%만성을형간염%IFN-α
Memory T cell%Chronic hepatitis B%IFN-α
目的 研究IFN-α在治疗慢性乙型肝炎患者中对记忆性T细胞亚群变化的影响,及其临床治疗效果与记忆性T细胞亚群变化的相关性.方法 57例接受IFN-α治疗的慢性乙型肝炎(CHB)患者,分别在治疗前(O周),及疗程期内不同时间点(12周和24周)抽取外周血,分离外周血单个核细胞(PBMCs)后加入抗体染色,流式细胞仪检测,统计学分析.结果 非活动性HBsAg携带者CD8效应记忆性T细胞(CD8+TEM)的比例显著高于未经IFN-α治疗的CHB患者;相反,CD8中心记忆性T细胞(CD8+TCM)的百分比则显著低于未经IFN-α治疗的CHB患者(P<0.05);有效组CD8+TEM百分比在IFN-α治疗前和治疗24周时均高于无效组,CD8+TCM百分比在IFN-α治疗前和治疗24周时则低于无效组(P<0.05);有效组IFN-α的剂量明显高于无效组.结论 病毒的清除与效应记忆性T细胞亚群比例可能有关,TEM亚群在数量上占优势可能对IFN-α治疗效果有益.
目的 研究IFN-α在治療慢性乙型肝炎患者中對記憶性T細胞亞群變化的影響,及其臨床治療效果與記憶性T細胞亞群變化的相關性.方法 57例接受IFN-α治療的慢性乙型肝炎(CHB)患者,分彆在治療前(O週),及療程期內不同時間點(12週和24週)抽取外週血,分離外週血單箇覈細胞(PBMCs)後加入抗體染色,流式細胞儀檢測,統計學分析.結果 非活動性HBsAg攜帶者CD8效應記憶性T細胞(CD8+TEM)的比例顯著高于未經IFN-α治療的CHB患者;相反,CD8中心記憶性T細胞(CD8+TCM)的百分比則顯著低于未經IFN-α治療的CHB患者(P<0.05);有效組CD8+TEM百分比在IFN-α治療前和治療24週時均高于無效組,CD8+TCM百分比在IFN-α治療前和治療24週時則低于無效組(P<0.05);有效組IFN-α的劑量明顯高于無效組.結論 病毒的清除與效應記憶性T細胞亞群比例可能有關,TEM亞群在數量上佔優勢可能對IFN-α治療效果有益.
목적 연구IFN-α재치료만성을형간염환자중대기억성T세포아군변화적영향,급기림상치료효과여기억성T세포아군변화적상관성.방법 57례접수IFN-α치료적만성을형간염(CHB)환자,분별재치료전(O주),급료정기내불동시간점(12주화24주)추취외주혈,분리외주혈단개핵세포(PBMCs)후가입항체염색,류식세포의검측,통계학분석.결과 비활동성HBsAg휴대자CD8효응기억성T세포(CD8+TEM)적비례현저고우미경IFN-α치료적CHB환자;상반,CD8중심기억성T세포(CD8+TCM)적백분비칙현저저우미경IFN-α치료적CHB환자(P<0.05);유효조CD8+TEM백분비재IFN-α치료전화치료24주시균고우무효조,CD8+TCM백분비재IFN-α치료전화치료24주시칙저우무효조(P<0.05);유효조IFN-α적제량명현고우무효조.결론 병독적청제여효응기억성T세포아군비례가능유관,TEM아군재수량상점우세가능대IFN-α치료효과유익.
Objective To investigate the influence of interferon-a therapy on CD8 T memory subsets in patients with chronic hepatitis B(CHB) and correlation between the effect of IFN-α and CD8 T memory subsets. Methods Blood samples from 57 patients with CHB were collected before treatment (0 week), at 12 weeks and 24 weeks of treatment with pegylated IFN-α. Assays were performed on freshly isolated peripheral blood mononuclear cells ( PBMCs). For phenotype analysis, All data were acquired on a flow cytometer instrument and prepared for analysis. Results A significantly higher frequency of CD8+ TEM and lower frequency of CD8+ TCM in inactive HBsAg carriers than that in CHB patients prior to treatment was observed (P <0.05). The proportion of CD8 + TCM was higher in group nonresponders than in group respond-ers, and the proportion of CD8 + TEM was lower in group nonresponders than in group responders (P < 0.05 ). The average dosage of IFN-α applied to patients with response was significantly higher than nonresponders. Conclusion The dominance of circulating effector memory T cells may be associated with elimination of viral infection, and possibly benefit for response to therapy with IFN-α.
