中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2008年
6期
365-368
,共4页
孙勤%李伶%杨刚毅%李仁哲%李钶%齐晓亚%李生兵%陈文雯%刘桦%唐毅%Guenther Boden
孫勤%李伶%楊剛毅%李仁哲%李鈳%齊曉亞%李生兵%陳文雯%劉樺%唐毅%Guenther Boden
손근%리령%양강의%리인철%리아%제효아%리생병%진문문%류화%당의%Guenther Boden
内脂素%胰岛素抗体%正常血糖高胰岛素钳夹技术
內脂素%胰島素抗體%正常血糖高胰島素鉗夾技術
내지소%이도소항체%정상혈당고이도소겸협기술
Visfatin%Insulin antibodics%Euglycemic-hyperinsul inemia clamp
目的 探讨内脂素基因过表达对高脂诱导胰岛素抵抗(IR)大鼠胰岛素敏感性的影响.方法 构建大鼠内脂素基因真核表达质粒,并用该质粒转染高脂喂养诱导的IR大鼠模型.在转染前后采用两次高胰岛素-正糖钳夹技术评价大鼠胰岛素敏感性的变化.结果 成功构建了内脂素真核表达载体pcDNA3.1内脂素.实验组大鼠在pcDNA3.1内脂素质粒注射后3 d血浆内脂素水平较注射前明显升高(2.19 ±0.36 vs 0.98±0.27,P＜0.01);在两次胰岛素钳夹中,pcDNA3.1内脂素质粒注射后葡萄糖输注率较注射前明显升高[(32.6 ±1.2)mg·kg-1·min-1 vs(24.0±1.2)mg·kg-1·min-1,P＜0.01];总胆固醇、高密度脂蛋白胆固醇则较质粒注射前明显降低[(2.36±0.22)mmol/Lvs(1.60±0.21)mmol/L和(1.41±0.24)mmol/L vs(0.88±0.11)mmol/L,均P＜0.05];在转染前后基础血糖和胰岛素水平无明显变化.结论 内脂素质粒转染使IR大鼠血浆内脂素水平升高,胰岛素敏感性增强.
目的 探討內脂素基因過錶達對高脂誘導胰島素牴抗(IR)大鼠胰島素敏感性的影響.方法 構建大鼠內脂素基因真覈錶達質粒,併用該質粒轉染高脂餵養誘導的IR大鼠模型.在轉染前後採用兩次高胰島素-正糖鉗夾技術評價大鼠胰島素敏感性的變化.結果 成功構建瞭內脂素真覈錶達載體pcDNA3.1內脂素.實驗組大鼠在pcDNA3.1內脂素質粒註射後3 d血漿內脂素水平較註射前明顯升高(2.19 ±0.36 vs 0.98±0.27,P＜0.01);在兩次胰島素鉗夾中,pcDNA3.1內脂素質粒註射後葡萄糖輸註率較註射前明顯升高[(32.6 ±1.2)mg·kg-1·min-1 vs(24.0±1.2)mg·kg-1·min-1,P＜0.01];總膽固醇、高密度脂蛋白膽固醇則較質粒註射前明顯降低[(2.36±0.22)mmol/Lvs(1.60±0.21)mmol/L和(1.41±0.24)mmol/L vs(0.88±0.11)mmol/L,均P＜0.05];在轉染前後基礎血糖和胰島素水平無明顯變化.結論 內脂素質粒轉染使IR大鼠血漿內脂素水平升高,胰島素敏感性增彊.
목적 탐토내지소기인과표체대고지유도이도소저항(IR)대서이도소민감성적영향.방법 구건대서내지소기인진핵표체질립,병용해질립전염고지위양유도적IR대서모형.재전염전후채용량차고이도소-정당겸협기술평개대서이도소민감성적변화.결과 성공구건료내지소진핵표체재체pcDNA3.1내지소.실험조대서재pcDNA3.1내지소질립주사후3 d혈장내지소수평교주사전명현승고(2.19 ±0.36 vs 0.98±0.27,P＜0.01);재량차이도소겸협중,pcDNA3.1내지소질립주사후포도당수주솔교주사전명현승고[(32.6 ±1.2)mg·kg-1·min-1 vs(24.0±1.2)mg·kg-1·min-1,P＜0.01];총담고순、고밀도지단백담고순칙교질립주사전명현강저[(2.36±0.22)mmol/Lvs(1.60±0.21)mmol/L화(1.41±0.24)mmol/L vs(0.88±0.11)mmol/L,균P＜0.05];재전염전후기출혈당화이도소수평무명현변화.결론 내지소질립전염사IR대서혈장내지소수평승고,이도소민감성증강.
Objective To investigate the effects of visfatin gene overexpression on insulin sensitivitv in insulin-resistant(IR)rats induced by high-fat diet.Methods The recombinant visfatin Dlasmid was constructed and transfected into IR rats induced by high-fat diet.The euglycemic hyperinsulinemic clamp experiments were performed for evaluation the change of insulin sensitivity before and after administration.Results The expression plasmid of visfatin were successfully constructed.After 3 days for plasmid injecting,plasma visfatin levels and glucose infusion rates were significantly increased(2.19±0.36 vs 0.98±0.27 and 32.6±1.2 vs 24.0±1.2 mg·kg-1·min-1,respectively,all P＜0.01),and total cholesterol and high-density lipoprotein cholesterol(HDL-C)were significantly decreased(2.36±0.22 vs 1.60±0.21 mmol/L and 1.41±0.24 vs 0.88±0.11 mmol/L,respectively,all P＜0.05)in high-fat diet rats.Conclusion The transfection of visfatin plasmid enhanced plasma visfatin level and improved insulin sensitivity in IR rats induced by high-fat diet.
