CAJ | 학술논문

目的研究胃癌组织中CD147和上皮型钙黏蛋白(E-cadherin)的表达情况及其与胃癌临床病理特征的关系.方法制备组织芯片,应用原位杂交和免疫组化技术检测220例胃癌组织和31例正常胃黏膜中CD147和E-cadherin的表达情况.结果 CD147mRNA、E-cadherin mRNA和E-cadherin蛋白在胃癌组织中的阳性表达率分别是50.5%、17.7%和15.5%,CD147与E-cadherin表达呈负相关,CD147 mRNA的表达与胃癌的肿瘤大小、TNM分期、浸润深度、脉管侵犯、淋巴结转移和远处转移相关;E-cadherin mBNA的表达与胃癌的TNM分期、浸润深度和脉管侵犯相关;E-cadherin蛋白的表达与肿瘤大小、TNM分期和脉管侵犯相关.CD147 mRNA阳性表达患者的平均生存时间和5年生存率明显低于阴性者,而E-cadherin mRNA和蛋白阳性者的平均生存时间和5年生存率明显高于阴性者.结论胃癌组织中,CD147表达上调,E-cadherin表达下调,且二者呈负相关;联合检测胃癌组织中CD147和E-cadherin的表达情况,可预测胃癌的浸润和转移,对指导临床治疗及预后评估有重要意义.
목적 연구위암조직중CD147화상피형개점단백(E-cadherin)적표체정황급기여위암림상병리특정적관계.방법 제비조직심편,응용원위잡교화면역조화기술검측220례위암조직화31례정상위점막중CD147화E-cadherin적표체정황.결과 CD147mRNA、E-cadherin mRNA화E-cadherin단백재위암조직중적양성표체솔분별시50.5%、17.7%화15.5%,CD147여E-cadherin표체정부상관,CD147 mRNA적표체여위암적종류대소、TNM분기、침윤심도、맥관침범、림파결전이화원처전이상관;E-cadherin mBNA적표체여위암적TNM분기、침윤심도화맥관침범상관;E-cadherin단백적표체여종류대소、TNM분기화맥관침범상관.CD147 mRNA양성표체환자적평균생존시간화5년생존솔명현저우음성자,이E-cadherin mRNA화단백양성자적평균생존시간화5년생존솔명현고우음성자.결론 위암조직중,CD147표체상조,E-cadherin표체하조,차이자정부상관;연합검측위암조직중CD147화E-cadherin적표체정황,가예측위암적침윤화전이,대지도림상치료급예후평고유중요의의.
Objective To investigate the expression of CD147 and E-cadherin in gastric carcinoma and their correlation with clinicopathological features. Methods The expression of CD147 and E-cadherin in gastric cancer tissue chip (TC) was detected by in situ hybridization (ISH) and immtmohistochemistry in 220 cases of gastric carcinoma and 31 cases with normal gastric mucosa. Results The expression rates of CD147 mRNA,E-cadberin mRNA and E-cadherin protein were 50.5%, 17.7% and 15.5%, respectively. The CD147 expression was negatively correlatod with E-cadberin expression. There was a siguificant relationship between CD147 mRNA expression and tumor diameter, TNM stage, invasion depth, vessel invasion, lymph node and distant metastasis. The E-cadherin mRNA expression was closely related with TNM stage, invasion depth and vascular invasion. There was a siguificant relationship between E-cadherin protein expression and tumor diameter, TNM stage and vascular invasion. The mean survival time in cases with CD147-positive expression was shorter than that in negative cases, while E-cadberin was in an opposite relationship. Conclusion In gastric carcinoma, up-regulation of CD147 and down-regulation of E-cadherin are in a negative correlation. The examination of both these two factors together is useful for predicting the invasion and metastasis of gastric cancer, therefore, can be used as a significant marker to direct clinic therapy and estimation of prognosis.