CAJ | 학술논문

采用水提醇沉工艺获得了水溶性野木瓜粗多糖(CCP),经过Sevag法脱蛋白,大孔树脂脱色及透析,得到野木瓜精多糖,再经DEAE-纤维素和Sepharose Cl-6B柱层析,从野木瓜中分离得到多糖组分CCP1,经过高效凝胶渗透色谱法(HPGPC)鉴定为均一组分,测定其相对分子量(MW)为2.879 ×106D.高效液相法测定CCP1的单糖组成为:鼠李糖、阿拉伯糖、果糖、甘露糖、葡萄糖,其摩尔比为0.034:0.228:0.045:0.055:0.638.补体结合实验表明,多糖CCP1在体外具有一定抗补体活性,并呈一定的剂量效应关系.
채용수제순침공예획득료수용성야목과조다당(CCP),경과Sevag법탈단백,대공수지탈색급투석,득도야목과정다당,재경DEAE-섬유소화Sepharose Cl-6B주층석,종야목과중분리득도다당조분CCP1,경과고효응효삼투색보법(HPGPC)감정위균일조분,측정기상대분자량(MW)위2.879 ×106D.고효액상법측정CCP1적단당조성위:서리당、아랍백당、과당、감로당、포도당,기마이비위0.034:0.228:0.045:0.055:0.638.보체결합실험표명,다당CCP1재체외구유일정항보체활성,병정일정적제량효응관계.
The crude water-soluble polysaccharides from Chaenomeles cathayensis fruits were isolated with hot water extraction followed by ethanol precipitation. The polysaccharides were deproteinized using Sevag method, decolorized by macroporous resin and then dialyzed. The fine water-soluble Chaenomeles cathayensis polysaccharides (CCP) were obtained and further fractionated to elute for the component CCP1 by anion exchange column chromatography on DEAE-cellulose. The purity of CCP1 was examined by gel filtration chromatography of Sepharose Cl-6B. The purified polysaccharide CCP1 was proved to be homogeneous by HPGPC and its molecular weight (MW) was 2.879×106 D. Analysis of monosaccharide composition by HPLC indicated that CCP1 consists of rhamnose, arabinose, fructose, mannitose, glucose and their molar ratio is 0.034:0.228:0.045:0.055:0.638. The main fraction CCP1 was subjected to the complement-fixation experiment. CCP1 expressed some anti-complementary activity in vitro and dosage-effect relationship between them existing.