时珍国医国药
時珍國醫國藥
시진국의국약
LISHIZHEN MEDICINE AND MATERIA MEDICA RESEARCH
2009年
7期
1645-1646
,共2页
张晓霞%陈安家%张一鸣%王洁%李霞%李进冉
張曉霞%陳安傢%張一鳴%王潔%李霞%李進冉
장효하%진안가%장일명%왕길%리하%리진염
毛细管气相色谱法%消肿止痛酊%樟脑%薄荷脑
毛細管氣相色譜法%消腫止痛酊%樟腦%薄荷腦
모세관기상색보법%소종지통정%장뇌%박하뇌
Capillary Gas chromatography%Xiaozhongzhitong Tincture%Camphor%Menthol
目的 建立测定消肿止痛酊中樟脑和薄荷脑含量的方法.方法 采用毛细管气相色谱法.色谱柱为PEG-20M石英毛细管柱(30 m×0.25 mm×0.25 μm);载气为氮气;正辛醇为内标;柱温160℃;氢火焰离子检测器;检测器和气化室的温度均为250℃;N2流速3 ml/min;H2流速30 ml/min;空气流速300 ml/min;尾吹气N2流速30 ml/min.结果 樟脑、薄荷脑和正辛醇能达到良好分离;樟脑的线性范围3.00~18.00 μg/ml(r=0.999 2),薄荷脑的线性范围3.20~19.20 μg/ml(r=0.999 5);樟脑的回收率98.5%~101.5%,薄荷脑的回收率97.6%~101.7%.结论 方法简便、准确、专属性强、重复性好,可用于消肿止痛酊的质量控制.
目的 建立測定消腫止痛酊中樟腦和薄荷腦含量的方法.方法 採用毛細管氣相色譜法.色譜柱為PEG-20M石英毛細管柱(30 m×0.25 mm×0.25 μm);載氣為氮氣;正辛醇為內標;柱溫160℃;氫火燄離子檢測器;檢測器和氣化室的溫度均為250℃;N2流速3 ml/min;H2流速30 ml/min;空氣流速300 ml/min;尾吹氣N2流速30 ml/min.結果 樟腦、薄荷腦和正辛醇能達到良好分離;樟腦的線性範圍3.00~18.00 μg/ml(r=0.999 2),薄荷腦的線性範圍3.20~19.20 μg/ml(r=0.999 5);樟腦的迴收率98.5%~101.5%,薄荷腦的迴收率97.6%~101.7%.結論 方法簡便、準確、專屬性彊、重複性好,可用于消腫止痛酊的質量控製.
목적 건립측정소종지통정중장뇌화박하뇌함량적방법.방법 채용모세관기상색보법.색보주위PEG-20M석영모세관주(30 m×0.25 mm×0.25 μm);재기위담기;정신순위내표;주온160℃;경화염리자검측기;검측기화기화실적온도균위250℃;N2류속3 ml/min;H2류속30 ml/min;공기류속300 ml/min;미취기N2류속30 ml/min.결과 장뇌、박하뇌화정신순능체도량호분리;장뇌적선성범위3.00~18.00 μg/ml(r=0.999 2),박하뇌적선성범위3.20~19.20 μg/ml(r=0.999 5);장뇌적회수솔98.5%~101.5%,박하뇌적회수솔97.6%~101.7%.결론 방법간편、준학、전속성강、중복성호,가용우소종지통정적질량공제.
Objective To establish a method for GC determination of Camphor and Menthol in Xiaozhongzhitong Tincture.Methods Capillary GC was adopted.The internal standard method was employed with octanol as internal standard.The GC system consisted of a flame ionization detector,PEG-20M capillary column(30 m×0.25 mm×0.25 μm),nitrogen as the carrier gas;oven temperature 160℃,detector temperature 250℃,N2:3 ml/min,H2:30 ml/min,Air:300ml/min,made up(N2):30 ml/min.Results Camphor and Menthol could be separated well under the chromatographic conditions.The linear range of Camphor was 3.00~18.00 μg/ml (r=0.999 2),and the linear range of the Menthol was 3.20~19.20 μg/ml(r=0.999 5);the recovery of Camphor was 98.5%~101.5%,and the recovery of Menthol was 97.6%~101.7%.Conclusion The method is simple,sensitive,accurate and has good repeatability,and it can be used to control the quality of Xiaozhongzhitong Tincture.