CAJ | 학술논문

基于聚合酶链式反应的变性梯度凝胶电泳(Polymerase chain reaction denaturing graaient gel electrophoresis,PCR-DGGE)技术作为研究微生物物种多样性和动态变化的分子检测工具之一,具有可靠性强、重复性好、方便快捷等优点.该技术无需传统的微生物分离培养,便能快速、准确地获取复杂样品中微生物的菌群多样性、动态性及其功能菌的遗传信息,被广泛用于环境生态学的研究中.该文概述了PCR-DGGE技术的基本原理,并对该技术的各个环节如DNA提取、PCR扩增、DGGE条件以及图谱染色等条件的优化进行探讨,提出可能出现的影响因素,并对该技术自身存在的局限性和应用前景进行了评价.
기우취합매련식반응적변성제도응효전영(Polymerase chain reaction denaturing graaient gel electrophoresis,PCR-DGGE)기술작위연구미생물물충다양성화동태변화적분자검측공구지일,구유가고성강、중복성호、방편쾌첩등우점.해기술무수전통적미생물분리배양,편능쾌속、준학지획취복잡양품중미생물적균군다양성、동태성급기공능균적유전신식,피엄범용우배경생태학적연구중.해문개술료PCR-DGGE기술적기본원리,병대해기술적각개배절여DNA제취、PCR확증、DGGE조건이급도보염색등조건적우화진행탐토,제출가능출현적영향인소,병대해기술자신존재적국한성화응용전경진행료평개.
As a reliable,reproducible rapid and inexpensive molecular method,PCR-DGGE had been proven to be a powerful tool for studying microbial communities and their dynamics changes.This technique was widely used in environmental ecology to analyze the diversity and dynamics of microbial communities,and to monitor functional bacteria without isolation and cultivation processes.In this paper,the principle,as well as the limitations and future perspectives of DGGE techniques for microbial ecology analysis were introduced,and the main causing factors addressed and discussed,such as DNA extraction,PCR amplification,DGGE conditions optimization.