CAJ | 학술논문

[目的]研究拟南芥莲座叶芥子油苷对聚乙二醇模拟干旱的响应.[方法]以PEG-6000模拟干旱胁迫,设置对照组和干旱处理组分别培养拟南芥Columbia生态型种子,并在处理后的第0、4、5、6、7天分别取2组拟南芥莲座叶进行生理指标和芥子油苷含量的测定.叶片相对含水量测定采用烘干称重法(水分占叶片鲜重的百分比);叶片相对电导率和丙二醛(MDA)浓度测定参照李合生的方法;采用高效液相色谱-质谱联用(HPLC-MS)系统进行芥子油苷的测定.[结果]干旱胁迫处理后营养生长时期的拟南芥幼苗莲座叶叶片的相对含水量下降,相对电导率(表征膜透性)及丙二醛浓度增加,受损害程度随处理时间的增加而增强.拟南芥莲座叶芥子油苷总量、脂肪族芥子油苷含量及吲哚族芥子油苷含量均在处理后第5天达到最大值,在处理后第6天迅速下降并低于对照组.各种芥子油苷含量变化存在差异,其中含量最高的4MSOB与芥子油苷总量变化趋势一致且差异显著,脂肪族芥子油苷响应干旱胁迫较吲哚族芥子油苷强烈.4MSOB等部分芥子油苷占芥子油苷总量的比例在干旱处理后发生变化并且与其本身含量变化相关.[结论]干旱胁迫确实对拟南芥莲座叶中芥子油苷总量、脂肪族芥子油苷及吲哚族芥子油苷的含量产生影响,使芥子油苷参与到植物对外界干旱胁迫的防御反应中,但长期的干旱胁迫不利于叶片中芥子油苷的积累.
[목적]연구의남개련좌협개자유감대취을이순모의간한적향응.[방법]이PEG-6000모의간한협박,설치대조조화간한처리조분별배양의남개Columbia생태형충자,병재처리후적제0、4、5、6、7천분별취2조의남개련좌협진행생리지표화개자유감함량적측정.협편상대함수량측정채용홍간칭중법(수분점협편선중적백분비);협편상대전도솔화병이철(MDA)농도측정삼조리합생적방법;채용고효액상색보-질보련용(HPLC-MS)계통진행개자유감적측정.[결과]간한협박처리후영양생장시기적의남개유묘련좌협협편적상대함수량하강,상대전도솔(표정막투성)급병이철농도증가,수손해정도수처리시간적증가이증강.의남개련좌협개자유감총량、지방족개자유감함량급신타족개자유감함량균재처리후제5천체도최대치,재처리후제6천신속하강병저우대조조.각충개자유감함량변화존재차이,기중함량최고적4MSOB여개자유감총량변화추세일치차차이현저,지방족개자유감향응간한협박교신타족개자유감강렬.4MSOB등부분개자유감점개자유감총량적비례재간한처리후발생변화병차여기본신함량변화상관.[결론]간한협박학실대의남개련좌협중개자유감총량、지방족개자유감급신타족개자유감적함양산생영향,사개자유감삼여도식물대외계간한협박적방어반응중,단장기적간한협박불리우협편중개자유감적적루.
[Objective] The paper aimed to study effects of drought stress simulated by PEG on glucosinolates content in Arabidopsis thaliana.[Method] Drought stress was simulated by PEG-6000,ecological seeds of Arabidopsis thaliana were cultivated by the control group and drought treatment group respectively,Physical signs of Arabidopsis thaliana and contents of glucosinolates were determined after 0,4,5,6,7 d treatment.[Result] The results showed that leaf water content of rosette leaves was obviously decreased,leaf relative conductivity (characterized by membrane permeability) and the concentration of MDA increased,the extent of damage increased with the increased time.Content of total glucosinolate,aliphatic glucosinolate and indole glucosinolate increased got their maximum after 5 days treatment,and rapidly decreased after 6 and 7 days of treatment,even much lower than the control group.Each kind of glucosinolate changed with difference from each other.4MSOB which made the most proportion of the total glucosinolate changed consistently with the total glucosinolate and difference significant.As a whole,aliphatic glucosinolates were more sensitive to drought than indole glucosinolate.The proportion of some kind glucosinolate,like 4MSOB varied had correlation with the content change.[Conclusion] Drought stress had an effects on the contents of total glucosinolate,aliphatic glucosinolate and indole glucosinolate,which made the glucosinolate participated in defense response of plant to the outside of drought stress,but long-term drought stress in leaves was not conducive to the accumulation of glucosinolates.