CAJ | 학술논문

目的探索一种损伤小、可靠、高效的小鼠气管内插管方法并滴定脂多糖制备急性肺损伤模型.方法 80只BALB/C小鼠按随机数字表法分为两组:脂多糖组(LPS组)和对照组(NS组).气管内插管成功后分别滴定脂多糖(3 mg/kg)和生理盐水(1.5 ml/kg),记录插管一次成功率、最终成功率、存活率.24 h后计数左肺肺泡灌洗液(BALF)中总细胞数,瑞氏染色进行细胞分类计数,BCA法测定总蛋白浓度,肺组织烘干后计算湿/干比,动脉血气分析测定血氧分压(PaO2)并计算氧合指数.结果改良式颈透视下插管一次成功率92.5％、最终成功率和存活率均为100％；LPS组BALF中总细胞数[(10.82 ±3.51)×105/ml vs (0.72 ±0.52)×105/ml,t=-6.294,P＜0.01]、多形核粒细胞占总细胞数比例[(93.93±1.77)％vs(2.2±0.91)％,t=-105.565,P＜0.01]、单个核粒细胞占总细胞数比例[(6.07±1.77)％vs(97.8±0.91)％,t=- 105.565,P＜0.01]、总蛋白浓度[ (0.49 ±0.13) mg/ml vs (0.29 ±0.11) mg/ml,t=-2.823,P＜0.05]、湿/干比值(4.60 ±0.18vs4.16±0.25,t=-4.793,P＜0.01)、动脉PaO2[(68.57±7.23)％ vs(87.00±6.33)％,t=4.571,P＜0.01]和氧合指数[(326.53±34.43) mmHg vs( 414.29±30.16) mmHg,t=4.571,P＜0.01]与NS组比较差异均有统计学意义.结论改良式小鼠颈透视下气管内插管成功率高、损伤小,且滴定LPS 3 mg/kg成功制备了急性肺损伤模型.
목적 탐색일충손상소、가고、고효적소서기관내삽관방법병적정지다당제비급성폐손상모형.방법 80지BALB/C소서안수궤수자표법분위량조:지다당조(LPS조)화대조조(NS조).기관내삽관성공후분별적정지다당(3 mg/kg)화생리염수(1.5 ml/kg),기록삽관일차성공솔、최종성공솔、존활솔.24 h후계수좌폐폐포관세액(BALF)중총세포수,서씨염색진행세포분류계수,BCA법측정총단백농도,폐조직홍간후계산습/간비,동맥혈기분석측정혈양분압(PaO2)병계산양합지수.결과 개량식경투시하삽관일차성공솔92.5％、최종성공솔화존활솔균위100％；LPS조BALF중총세포수[(10.82 ±3.51)×105/ml vs (0.72 ±0.52)×105/ml,t=-6.294,P＜0.01]、다형핵립세포점총세포수비례[(93.93±1.77)％vs(2.2±0.91)％,t=-105.565,P＜0.01]、단개핵립세포점총세포수비례[(6.07±1.77)％vs(97.8±0.91)％,t=- 105.565,P＜0.01]、총단백농도[ (0.49 ±0.13) mg/ml vs (0.29 ±0.11) mg/ml,t=-2.823,P＜0.05]、습/간비치(4.60 ±0.18vs4.16±0.25,t=-4.793,P＜0.01)、동맥PaO2[(68.57±7.23)％ vs(87.00±6.33)％,t=4.571,P＜0.01]화양합지수[(326.53±34.43) mmHg vs( 414.29±30.16) mmHg,t=4.571,P＜0.01]여NS조비교차이균유통계학의의.결론 개량식소서경투시하기관내삽관성공솔고、손상소,차적정LPS 3 mg/kg성공제비료급성폐손상모형.
Objective To explore a minimally invasive,reliable,and efficient method for endotracheal intubation to instill lipopolysaccharide (LPS) for preparation of acute lung injury (ALI) in mice.Methods A total of 80 BALB/C mice was randomly selected into LPS group ( n =40) and control group (Normal saline,NS; n =40).After a successfully endotracheal intubation,each mouse was instilled by LPS (3 mg/kg) in LPS group,and NS ( 1.5 ml/kg) in NS group,respectively.The one-time success rate and final success rate of the endotracheal intubation,and survival rate were recorded.After 24 hours,the total number of cells in bronchoalveolar lavage fluid (BALF) of left lung was counted with light microscope.The cells were classified and counted after Wright's stain.Total protein concentration in BALF was assayed with a BCA kit.Wet/dry value was calculated after the lung became dry.Artery blood PaO2 was tested and the oxygenation index was counted.Results Compared to NS group,the LPS group had the one-time success rate 92.5％,and the final success rate 100％,survival rate 100％,the total number of cells [ ( 10.82±3.51) ×105/mlvs (0.72±0.52) ×105/ml.t =-6.294 P ＜0 01] the rate of polymorphonulear leukocytes in total cells [ (93.93 ± 1.77) ％ vs (2.2 ± 0.91 ) ％,t =- 105.565,P ＜ 0.01 ],the rate of mononuclear leukocytes in total cells[ (6.07 ± 1.77)％ vs (97.8 ±0.91 )％,t =- 105.565,P ＜0.01 ],total protein concentration[ (0.49 ± 0.13 ) mg/ml vs (0.29 ± 0.11 ) mg/ml,t =- 2.823,P ＜ 0.05 ],W/D ratio(4.60 ±0.18 vs 4.16 ±0.25,t =-4.793,P ＜0.01 ),PaO2[ (68.57 -±7.23)％ vs(87.00 ±6.33 )％,t =4.571,P ＜ 0.01 ],and oxygenation index [ (326.53 ± 34.43 )mmHg vs (414.29 ± 30.16)mmHg,t =4.571,P ＜0.01 ].Conclusions Improved method for endotracheal intubation has high success rate and minimal injury,and instillation of LPS (3 mg/kg)can induce mice ALI successfully.