CAJ | 학술논문

目的比较牙龈结合上皮(junctional epithelium,JE)与口腔龈上皮(oral epithelium,OE)细胞的生长附着特性,观察附着结构的形成过程,为牙周附着的形成提供实验依据.方法人JE和OE取自正畸或阻生原因拔除的牙周健康牙,采用酶消化法和无血清角质细胞培养液分离培养细胞.将5×108个/L JE细胞1ml接种到放置于24孔板的预处理5mm×3mm×1mm牙骨质片上(每孔3片,共21片),于37℃ 5%CO2培养箱中复合培养,1～14d内取样本,透射电镜下观察附着结构的形成;同法以人OE细胞-牙骨质片复合培养作对照研究.结果 JE细胞形态多样,排列散乱,CKl9表达强阳性;OE细胞呈多边形或角形,"铺路石"状排列,仅少量OE细胞CK19阳性.JE细胞-牙骨质片复合培养1～3d,牙片上附着细胞少,9d时接触牙片的细胞膜局部出现少量电子致密沉积物,11～14d出现复层生长,与牙面接触处可见半桥粒样结构;而OE细胞-牙骨质片复合培养1～3 d牙骨质片上细胞较多,部分已伸展成扁平形,7 d时细胞膜和牙面间出现大量颗粒状电子致密沉积物,9 d时细胞呈复层生长,可见半桥粒样结构.结论人JE细胞是一种不同于OE细胞的未成熟、低分化细胞,在同样条件下体外培养的JE细胞在牙片上的生长和附着均较OE细胞慢,其对牙面的附着强度值得深入研究.
목적 비교아간결합상피(junctional epithelium,JE)여구강간상피(oral epithelium,OE)세포적생장부착특성,관찰부착결구적형성과정,위아주부착적형성제공실험의거.방법 인JE화OE취자정기혹조생원인발제적아주건강아,채용매소화법화무혈청각질세포배양액분리배양세포.장5×108개/L JE세포1ml접충도방치우24공판적예처리5mm×3mm×1mm아골질편상(매공3편,공21편),우37℃ 5%CO2배양상중복합배양,1～14d내취양본,투사전경하관찰부착결구적형성;동법이인OE세포-아골질편복합배양작대조연구.결과 JE세포형태다양,배렬산란,CKl9표체강양성;OE세포정다변형혹각형,"포로석"상배렬,부소량OE세포CK19양성.JE세포-아골질편복합배양1～3d,아편상부착세포소,9d시접촉아편적세포막국부출현소량전자치밀침적물,11～14d출현복층생장,여아면접촉처가견반교립양결구;이OE세포-아골질편복합배양1～3 d아골질편상세포교다,부분이신전성편평형,7 d시세포막화아면간출현대량과립상전자치밀침적물,9 d시세포정복층생장,가견반교립양결구.결론 인JE세포시일충불동우OE세포적미성숙、저분화세포,재동양조건하체외배양적JE세포재아편상적생장화부착균교OE세포만,기대아면적부착강도치득심입연구.
Objective To compare the attachment and growth characteristics between human junctional epithelium(JE)and oral epithelium cells.Methods The healthy JE biopsies were derived from the human teeth extracted due to impaction or orthodontic purpose.Enzyme digestion was used to isolate JE cells,which were then cultured in DKGM.The co-culture model of JE cell-tooth slice was buih up by adding 3 decalcification cementum slices(5mm×3mm×1mm)into sterilized plate containing 1ml of JE cells (5×108/L),21 slices all together,and incubated in an atmosphere containing 5% CO2 at 37℃ for 1-14days.The attachment structrure was observed under transmission electron microscope,and the OE cells was used as control.Results The human JE cells were polymorphous in shape and CKl9 posotive,while OE eells were consisted of equal and closely packed epithelial-like cells in a paving stone arrangement,and CK19 was only strained in a few cells.There were a few cells in JE-slice when co-cultured for 1-3 days.and electron dense plaques on the JE cell surface of the attached slice were observed at 9 days.and 2-3 layer of JE cells and hemidesmosome-like structure formed within 11-14 days.There were more OE cells within 1-3 days,electron dense plaques appeared at 7 days,and stratified epithelium and hemidesmosome-like structure formed in OE-slice at 9 days.Conclusions The cultured JE cells were immature and lower differentiated epithelial cells which were different from OE cells.Under the same condition the growth and attachment of JE cells on the cementum slice surface were slower than that of OE cells.Their attachment strength needs further study.