CAJ | 학술논문

目的研究骨质疏松骨骼局部注射辛伐他汀刺激成骨的效果,探索疏松骨骼局部给药预防脆性骨折的治疗方法.方法 36只3月龄雌性SD大鼠双侧卵巢切除后加低钙饮食3个月,制备大鼠骨质疏松模型.实验大鼠随机分为3组,每组12只,分别在实验大鼠的左侧股骨髓腔内单次注射辛伐他汀溶液5mg、10 mg,对照组单纯注射空白载体.分别在术后1个月及术后5个月每组随机处死半数大鼠(n=6)并取材.双能X线骨密度仪测定骨密度、Micro-CT扫描并定量分析骨组织形态改变、骨生物力学测试研究骨骼力学性能的变化.结果辛伐他汀局部注射后1个月和5个月,辛伐他汀注射组的骨密度、骨微结构参数如骨皮质厚度、骨小梁密度及连接率明显优于对照组,股骨髁及股骨颈的力学性能明显高于对照组.单次注射辛伐他汀的局部骨强化效果至少持续5个月,对照组骨量则持续丢失,力学性能持续降低.结论疏松骨骼单次注射小剂量辛伐他汀可强效而持久地促进皮质骨形成及骨小梁改建,改善骨骼微结构,增加骨密度及骨强度,可作为强化局部、防治骨质疏松骨折的新选择.
목적 연구골질소송골격국부주사신벌타정자격성골적효과,탐색소송골격국부급약예방취성골절적치료방법.방법 36지3월령자성SD대서쌍측란소절제후가저개음식3개월,제비대서골질소송모형.실험대서수궤분위3조,매조12지,분별재실험대서적좌측고골수강내단차주사신벌타정용액5mg、10 mg,대조조단순주사공백재체.분별재술후1개월급술후5개월매조수궤처사반수대서(n=6)병취재.쌍능X선골밀도의측정골밀도、Micro-CT소묘병정량분석골조직형태개변、골생물역학측시연구골격역학성능적변화.결과 신벌타정국부주사후1개월화5개월,신벌타정주사조적골밀도、골미결구삼수여골피질후도、골소량밀도급련접솔명현우우대조조,고골과급고골경적역학성능명현고우대조조.단차주사신벌타정적국부골강화효과지소지속5개월,대조조골량칙지속주실,역학성능지속강저.결론 소송골격단차주사소제량신벌타정가강효이지구지촉진피질골형성급골소량개건,개선골격미결구,증가골밀도급골강도,가작위강화국부、방치골질소송골절적신선택.
Objective To explore the bone anabolic effects after a single local injection of simvastatin into femoral cavities of osteoporotic rats.Methods Thirty-six female SD rats (3 months old,body weight 250-300 g) were ovariectomized (OVX) and low-calcium-diet fed for 3 months,OVX rats were randomized into 3 groups (n=12).Left femurs of group A,B and C were injected with 0,5 and 10 mg simvastatin,respectively.Half of the rats in each group were randomly euthanized separately 1 and 5 months after simvastatin injection.Left femurs were taken out for bone mineral density (BMD) assessment with dual energy X-ray absorptiometry,bone histomorphometic changes were analysized by Micro-CT,and two kinds of biomechanical tests were used to evaluate the osteogenic effects.Results One and five months after injection,BMD in mid-diaphysis significantly increased in simvastatin-injected groups compared to the control group.For Micro-CT analysis,significant increase in total bone volume/total tissue volume,cortical wall thickness,trabecular thickness,trabecular number,and a significant decrease in trabecular spacing were observed in simvastatin-injected groups compared to the control group.For both biomechanics (the three-pointbreaking test of condyles and axial compressive testing of proximal femur),the values were significantly higher in simvastatin-injected groups than the control group.Conclusion Local simvastatin treatment showed a positive effect on improving mechanical strength,structure of osteopenic femurs and BMD.Our findings may provide a new strategy for the prevention and treatment of osteoporosis,especially for osteoporotic fractures.