中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2012年
6期
708-712
,共5页
曾宪明%崔耀梅%程慧娴%朱运河%高玮%曾秋婷%段满林%徐建国
曾憲明%崔耀梅%程慧嫻%硃運河%高瑋%曾鞦婷%段滿林%徐建國
증헌명%최요매%정혜한%주운하%고위%증추정%단만림%서건국
离子通道%质子%脑%再灌注损伤
離子通道%質子%腦%再灌註損傷
리자통도%질자%뇌%재관주손상
Ion channels%Protons%Brain%Reperfusion injury
目的 评价不同剂量酸敏感离子通道1a特异性阻滞剂PcTx1对大鼠全脑缺血再灌注损伤的影响.方法 成年雄性SD大鼠60只,体重250～300 g,采用随机数字表法,将大鼠随机分为6组(n=10):假手术组(S组)、脑缺血再灌注组(I/R组)和不同剂量PcTx1组(P1组、P2组、P3组和P4组).I/R组、P1组、P2组、P3组和P4组采用改良的Pulsinelli四血管阻断法建立大鼠全脑缺血再灌注损伤模型,再灌注即刻P1组、P2组、P3组和P4组分别经侧脑室注射10、25、50、500 ng/ml PcTx1,I/R 组给予等容量6 μl双蒸水.于再灌注24h时,6组各取6只大鼠,取海马组织,测定还原型谷胱甘肽(GSH)、丙二醛(MDA)和一氧化氮(NO)的含量、结构型一氧化氮合酶(cNOS)和诱导型一氧化氮合酶( iNOS)的活性；于再灌注72h时,6组各取4只大鼠、取海马组织,光镜下观察海马CAI区病理学结果.结果 与S组比较,其它5组海马组织GSH含量降低,MDA和NO的含量、cNOS和iNOS的活性升高(P＜0.05或0.01)；与I/R组比较,B组、P3组和P4组海马组织GSH含量升高,海马组织MDA和NO的含量、cNOS和iNOS的活性降低(P＜0.05或0.01)；与P1组比较,P2组、P3组和P4组海马组织GSH含量升高,海马组织MDA含量和cNOS的活性降低,P3组和P4组海马组织NO含量和iNOS活性降低(P＜0.05或0.01);与P2组比较,P3组和P4组海马组织iNOS活性降低(P＜0.05或0.01).P3组和P4组海马组织CA1区损伤程度明显轻于I/R组和P1组.结论 侧脑室注射25、50、500 ng/ml(6μl)PcTx1可减轻大鼠全脑缺血再灌注损伤,50 ng/ml(6μl)是较为适合的剂量.
目的 評價不同劑量痠敏感離子通道1a特異性阻滯劑PcTx1對大鼠全腦缺血再灌註損傷的影響.方法 成年雄性SD大鼠60隻,體重250～300 g,採用隨機數字錶法,將大鼠隨機分為6組(n=10):假手術組(S組)、腦缺血再灌註組(I/R組)和不同劑量PcTx1組(P1組、P2組、P3組和P4組).I/R組、P1組、P2組、P3組和P4組採用改良的Pulsinelli四血管阻斷法建立大鼠全腦缺血再灌註損傷模型,再灌註即刻P1組、P2組、P3組和P4組分彆經側腦室註射10、25、50、500 ng/ml PcTx1,I/R 組給予等容量6 μl雙蒸水.于再灌註24h時,6組各取6隻大鼠,取海馬組織,測定還原型穀胱甘肽(GSH)、丙二醛(MDA)和一氧化氮(NO)的含量、結構型一氧化氮閤酶(cNOS)和誘導型一氧化氮閤酶( iNOS)的活性；于再灌註72h時,6組各取4隻大鼠、取海馬組織,光鏡下觀察海馬CAI區病理學結果.結果 與S組比較,其它5組海馬組織GSH含量降低,MDA和NO的含量、cNOS和iNOS的活性升高(P＜0.05或0.01)；與I/R組比較,B組、P3組和P4組海馬組織GSH含量升高,海馬組織MDA和NO的含量、cNOS和iNOS的活性降低(P＜0.05或0.01)；與P1組比較,P2組、P3組和P4組海馬組織GSH含量升高,海馬組織MDA含量和cNOS的活性降低,P3組和P4組海馬組織NO含量和iNOS活性降低(P＜0.05或0.01);與P2組比較,P3組和P4組海馬組織iNOS活性降低(P＜0.05或0.01).P3組和P4組海馬組織CA1區損傷程度明顯輕于I/R組和P1組.結論 側腦室註射25、50、500 ng/ml(6μl)PcTx1可減輕大鼠全腦缺血再灌註損傷,50 ng/ml(6μl)是較為適閤的劑量.
목적 평개불동제량산민감리자통도1a특이성조체제PcTx1대대서전뇌결혈재관주손상적영향.방법 성년웅성SD대서60지,체중250～300 g,채용수궤수자표법,장대서수궤분위6조(n=10):가수술조(S조)、뇌결혈재관주조(I/R조)화불동제량PcTx1조(P1조、P2조、P3조화P4조).I/R조、P1조、P2조、P3조화P4조채용개량적Pulsinelli사혈관조단법건립대서전뇌결혈재관주손상모형,재관주즉각P1조、P2조、P3조화P4조분별경측뇌실주사10、25、50、500 ng/ml PcTx1,I/R 조급여등용량6 μl쌍증수.우재관주24h시,6조각취6지대서,취해마조직,측정환원형곡광감태(GSH)、병이철(MDA)화일양화담(NO)적함량、결구형일양화담합매(cNOS)화유도형일양화담합매( iNOS)적활성；우재관주72h시,6조각취4지대서、취해마조직,광경하관찰해마CAI구병이학결과.결과 여S조비교,기타5조해마조직GSH함량강저,MDA화NO적함량、cNOS화iNOS적활성승고(P＜0.05혹0.01)；여I/R조비교,B조、P3조화P4조해마조직GSH함량승고,해마조직MDA화NO적함량、cNOS화iNOS적활성강저(P＜0.05혹0.01)；여P1조비교,P2조、P3조화P4조해마조직GSH함량승고,해마조직MDA함량화cNOS적활성강저,P3조화P4조해마조직NO함량화iNOS활성강저(P＜0.05혹0.01);여P2조비교,P3조화P4조해마조직iNOS활성강저(P＜0.05혹0.01).P3조화P4조해마조직CA1구손상정도명현경우I/R조화P1조.결론 측뇌실주사25、50、500 ng/ml(6μl)PcTx1가감경대서전뇌결혈재관주손상,50 ng/ml(6μl)시교위괄합적제량.
Objective To explore the effects of different doses of PcTx1,a specific blocker of acid-sensing ion channel 1a,on global cerebral ischemia/repedfusion (I/R) injury in rats,Methods Sixty adult male Sprague Dawley rats (weighing 250-300 g) were randomly divided into 6 grups ( n =10 each):sham operation group (group S),I/R group,different doses of PcTx1 ( 10 ng/ml,group P1 ; 25 ng/ml,group P2 ; 50 ng/ml,group P3 ;and 500 ng/ml,group P4 ) groups.Global cerebral ischemia was induced by the modified procedure of Pulsinelli 4-vessel occlusion.In groups P1,P2,P3 and P4,different doses of PcTx1 ( 10,25,50 and 500 ng/ml),6 μl each,were respectively injected into the lateral cerebral ventricle at the initiation of reperfusion,while equal volume of double distilled water was injected instead in group I/R.Six rats in each group were sacrificed at 24 h of reperfusion,and the brains were immediately removed,Thereafter,the contents of malondialdehyde (MDA),reduced glutathione (GSH) and ritric oxide (NO),the activities of constitutive NO synthase (eNOS) snd inducible NO synthase (iNOS) were detected in hippocampus.Four rats in each group were sacrificed at 72 h of reperfusion,and hematoxylin and eosin staining was used to observe the pathomorphological changes of the hippocampal neurons.Results Compared with group S,the other groups showed decreases in the contents of GSH,while increases in the contents of MDA and NO and the activities of cNOS and iNOS ( P ＜ 0.05 or 0.01 ).The contents of GSH increased,while the contents of MDA and NO and the activities of cNOS and iNOS decreased in groups P2,P3 and P4 compared with group I/R ( P ＜ 0.05 or 0.01).Compared with group P1,the contents of GSH increased,the contents of MDA and the activities of cNOS decreased in groups P2,P3 and P4,and the contents of NO and the activities of iNOS decreased in groups P3 and P4 ( P ＜ 0.05 or 0.01 ).Compared with group P2,the activities of iNOS decreased in groups P3 and P4(P ＜ 0.05 or 0.01).The damage to neurons in hippocampal CAI was severe in groups I/R and P1,but it was attenuated in groups P3 and P4.Conclusion PcTx1 25,50 and 500 ng/ml (6 μl)injected into lateral cerebral ventricle can attenuate global cerebral I/R injury in rats,and the dose 50 ng/ml (6 μl) is more suitable.
