CAJ | 학술논문

目的评价根动脉注射氯胺酮对犬脊髓的影响.方法健康成年犬20只,雌雄不拘,体重12～18 kg-随机分为2组(n=10):对照组(C组)和氯胺酮组(K组).经肋间后动脉,向根动脉方向置管至根动脉分支处,C组注射生理盐水2 ml,K组注射氯胺酮200 mg(用生理盐水稀释至2 ml).药物注射完毕后3 h,取根动脉相对应的脊髓节段,切片,分别行HE染色和尼氏染色,光镜下观察脊髓组织病理学结果,计数阴性神经元(不含有尼氏小体的神经元);采用免疫组化法测定神经元特异性烯醇化酶、S100β蛋白和Tau蛋白的表达水平.结果两组脊髓神经元形态正常,尼氏小体丰富,均未见阴性神经元.两组脊髓神经元特异性烯醇化酶、S100β蛋白和Tau蛋白的表达比较差异无统计学意义(P＞0.05).结论根动脉注射氯胺酮不会导致犬脊髓损伤.
목적 평개근동맥주사록알동대견척수적영향.방법 건강성년견20지,자웅불구,체중12～18 kg-수궤분위2조(n=10):대조조(C조)화록알동조(K조).경륵간후동맥,향근동맥방향치관지근동맥분지처,C조주사생리염수2 ml,K조주사록알동200 mg(용생리염수희석지2 ml).약물주사완필후3 h,취근동맥상대응적척수절단,절편,분별행HE염색화니씨염색,광경하관찰척수조직병이학결과,계수음성신경원(불함유니씨소체적신경원);채용면역조화법측정신경원특이성희순화매、S100β단백화Tau단백적표체수평.결과 량조척수신경원형태정상,니씨소체봉부,균미견음성신경원.량조척수신경원특이성희순화매、S100β단백화Tau단백적표체비교차이무통계학의의(P＞0.05).결론근동맥주사록알동불회도치견척수손상.
Objective To investigate the effect of ketamine injected via the radicular arteries on spinal cord. Methods Twenty healthy mongrel dogs of both sexes weighing 12-18 kg were randomly divided into 2 groups ( n = 10 each): control group (group C) and ketamine group (group K). The animals were anesthetized with intravenous pentobarbital 30-35 mg/kg, fentanyl 50-100 μg and vecuronium 0.2 mg/kg and maintained with propofol ically ventilated after tracheal intubation. A catheter was inserted into T8 poster intercostal artery and advanced toward the opening of radicular artery which supplies the spinal cord. Ketamine 100 mg (in 2 ml of normal saline)was injected via the catheter in group K. Three hours after ketamine administration, the animals were sacrificed. A 1.5 cm long segment of spinal cord at the level of T8 was removed for microscopic examination and determination of the expression of NSE, S100β and Tau protein by immuno-histochemistry. Results There was no significant difference in the number of Nissl' s staining-negative neuronal cells and the expression of NSE, S100β and Tau protein in the spinal cord between the 2 groups ( P ＞ 0.05 ). Conclusion Ketamine injected via the radicular arteries does not induce spinal cord injury.