CAJ | 학술논문

目的确定2006年11月广西大新县大范围腹泻暴发性流行的病原及其分子生物学特点.方法进行流行病学个案调查和粪便标本的实验室检测,用ELISA试剂盒进行A组轮状病毒的检测,对检测阳性标本进行轮状病毒分型并对其中4份检测阳性轮状病毒的vfy7全基因扩增.结果采集的64份腹泻患者粪便标本中共有30份检测为轮状病毒阳性,总阳性率为46.9%,此次暴发的流行病学和临床特征也符合轮状病毒腹泻特征,RT-PCR进行G/P分型显示为G1P[8]型轮状病毒,VP7氨基酸显示第68位氨基酸改变.结论 G1P[8]型A组轮状病毒是此次腹泻暴发的病原.值得注意的是,此次A组轮状病毒暴发累及儿童和成人.
목적 학정2006년11월엄서대신현대범위복사폭발성류행적병원급기분자생물학특점.방법진행류행병학개안조사화분편표본적실험실검측,용ELISA시제합진행A조륜상병독적검측,대검측양성표본진행륜상병독분형병대기중4빈검측양성륜상병독적vfy7전기인확증.결과 채집적64빈복사환자분편표본중공유30빈검측위륜상병독양성,총양성솔위46.9%,차차폭발적류행병학화림상특정야부합륜상병독복사특정,RT-PCR진행G/P분형현시위G1P[8]형륜상병독,VP7안기산현시제68위안기산개변.결론 G1P[8]형A조륜상병독시차차복사폭발적병원.치득주의적시,차차A조륜상병독폭발루급인동화성인.
Objective To analyze epidemiological characters of an outbreak of rotavirus diarrhea in Daxing county, Guangxi province. Methods Rotavirus-positive specimens were identified by ELBA kit. G/P typing assays were confirmed with multiplex seminested RT-PCR. Full-length VP7 genes of 4 positive specimens were amplified and analyzed. Results 30 cases of Rotavirus-positive were identified from 64 specimens. The attack rate was 46.9% , and G/P typing was G1P[8]. A change of VP7 amino acid residue is at positions 68.Conclusion G1P[8] rotavirus is the etiologic agents of this diarrhea outbreak. In addition, adults were included in this outbreak.