肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2012年
4期
259-262
,共4页
刘文操%冯建宏%李彩霞%翟金俊%张沁莲
劉文操%馮建宏%李綵霞%翟金俊%張沁蓮
류문조%풍건굉%리채하%적금준%장심련
肺肿瘤%腺癌%A549%乙酰胆碱%长春新碱
肺腫瘤%腺癌%A549%乙酰膽堿%長春新堿
폐종류%선암%A549%을선담감%장춘신감
Lung neoplasms%Adenocarcinoma%A549%Acetylcholine%Vincristine
目的 探讨乙酰胆碱联合长春新碱( VCR)对肺腺癌细胞生长及化疗的影响,检测其是否通过影响bcl-2及p53发挥作用.方法 将乙酰胆碱单独或与VCR(浓度为IC50)联合作用于肺腺癌细胞株A549,测定其对A549细胞的抑制率.用免疫组织化学法及Western blot法测定乙酰胆碱联合VCR对bcl-2及p53蛋白表达的影响.结果 0.1、1、10 μmol/L的乙酰胆碱对A549细胞的抑制率分别为( 0.050±0.032)％、(0.101±0.021)％、(0.169±0.015)％；0.1、1、10μmol/L的乙酰胆碱分别与0.2 μg/ml VCR联合,对肿瘤细胞的抑制率分别为(0.529±0.023)％、(0.545 ±0.011)％、(0.589±0.015)％,与VCR组比较差异均有统计学意义(q'值分别为1.09、1.37、1.83,均P＜0.05),0.1～10 μmol/L的乙酰胆碱单独作用于A549细胞表现出浓度依赖性的抑制作用,与VCR联合后显著提高A549对VCR的敏感性(P＜0.05)；此外,联合用药后各组bcl-2及p53蛋白表达均降低(P＜0.05).结论 乙酰胆碱单独及联合VCR均能抑制A549细胞的生长,其机制可能是乙酰胆碱与VCR协同或相加作用影响了bcl-2及p53蛋白的表达.
目的 探討乙酰膽堿聯閤長春新堿( VCR)對肺腺癌細胞生長及化療的影響,檢測其是否通過影響bcl-2及p53髮揮作用.方法 將乙酰膽堿單獨或與VCR(濃度為IC50)聯閤作用于肺腺癌細胞株A549,測定其對A549細胞的抑製率.用免疫組織化學法及Western blot法測定乙酰膽堿聯閤VCR對bcl-2及p53蛋白錶達的影響.結果 0.1、1、10 μmol/L的乙酰膽堿對A549細胞的抑製率分彆為( 0.050±0.032)％、(0.101±0.021)％、(0.169±0.015)％；0.1、1、10μmol/L的乙酰膽堿分彆與0.2 μg/ml VCR聯閤,對腫瘤細胞的抑製率分彆為(0.529±0.023)％、(0.545 ±0.011)％、(0.589±0.015)％,與VCR組比較差異均有統計學意義(q'值分彆為1.09、1.37、1.83,均P＜0.05),0.1～10 μmol/L的乙酰膽堿單獨作用于A549細胞錶現齣濃度依賴性的抑製作用,與VCR聯閤後顯著提高A549對VCR的敏感性(P＜0.05)；此外,聯閤用藥後各組bcl-2及p53蛋白錶達均降低(P＜0.05).結論 乙酰膽堿單獨及聯閤VCR均能抑製A549細胞的生長,其機製可能是乙酰膽堿與VCR協同或相加作用影響瞭bcl-2及p53蛋白的錶達.
목적 탐토을선담감연합장춘신감( VCR)대폐선암세포생장급화료적영향,검측기시부통과영향bcl-2급p53발휘작용.방법 장을선담감단독혹여VCR(농도위IC50)연합작용우폐선암세포주A549,측정기대A549세포적억제솔.용면역조직화학법급Western blot법측정을선담감연합VCR대bcl-2급p53단백표체적영향.결과 0.1、1、10 μmol/L적을선담감대A549세포적억제솔분별위( 0.050±0.032)％、(0.101±0.021)％、(0.169±0.015)％；0.1、1、10μmol/L적을선담감분별여0.2 μg/ml VCR연합,대종류세포적억제솔분별위(0.529±0.023)％、(0.545 ±0.011)％、(0.589±0.015)％,여VCR조비교차이균유통계학의의(q'치분별위1.09、1.37、1.83,균P＜0.05),0.1～10 μmol/L적을선담감단독작용우A549세포표현출농도의뢰성적억제작용,여VCR연합후현저제고A549대VCR적민감성(P＜0.05)；차외,연합용약후각조bcl-2급p53단백표체균강저(P＜0.05).결론 을선담감단독급연합VCR균능억제A549세포적생장,기궤제가능시을선담감여VCR협동혹상가작용영향료bcl-2급p53단백적표체.
Objective To study the effects of acetylcholine combined with vincristine (VCR) on A549 cells, furthermore to study the influence that is due to affecting the expression of the two proteins bcl-2 and p53. Methods MTT assay was used to analyze growth inhibition effect of acetylcholine alone and combined with IC50VCR on A549 cells.The expression of bcl-2 and p53 was measured by immunohistological chemistry technique(IHC) and Western blot.Results The inhibition rates for A549 cells with 0.1,1 and 10 μmol/L acetylcholine were (0.050±0.032)％,(0.101±0.021)％,(0.169±0.015)％.The inhibition rates for tumor cells with 0.1,1 and 10 μmol/L acetylcholine joint 0.2 μg/ml VCR were (0.529±0.023)％,(0.545±0.011)％,(0.589±0.015)％,the difference was statistically significant compared with the VCR group (q'values were 1.09,1.37,1.83,P＜0.05).Acetylcholine alone exerted inhibitory effect on A549 cells in a concentration dependent manner and significanrtly enhanced its sensitivity to VCR (P＜0.05).acetylcholibne (0.1-10 μmol/L)combined with IC50VCR decrased the expression of bcl-2 and p53 (P＜0.05).Conclusion Acetylcholine alone and combined with VCR can inhibit the growth of A549 cells. Significant synergistic effect between acetylcholine and VCR is found in inducing cell apoptosis by changing the expression of bcl-2 and p53.
