CAJ | 학술논문

目的建立HLA-Ⅰ积累表型频率(CPF)的空间预测系统,旨在指导中国疫苗学家设计受HLA限制的多表位疫苗(包括DNA疫苗),并评价或预测根据不同HLA-Ⅰ组合形式设计的多表位疫苗(或DNA疫苗)在中国不同地区的免疫效果.方法通过构建中国境内人群HLA-Ⅰ基因空间数据库,利用"克立格"技术建立CPF空间预测系统.结果根据疫苗设计目的,设计者可利用该系统预测和估计特定地理位置上人群的CPF,包括根据单个HLA座位(HLA-A或HLA-B)上的等位基因而设计的多表位疫苗和根据两个HLA基因座位(HLA-A和HLA-B)上的等位基因而设计的多表位疫苗在特定地理位置上人群的CPF值,从而估计或预测拟研制的或已研制的多表位疫苗在中国不同地理位置上人群中的免疫效果.结论该预测系统可用于:①鉴别任何中国群体的期望CPF百分比,为设计疫苗组分提供依据;②在特定地理位置的人群中,预测限制性表位已知的多表位疫苗的理论免疫应答率;③在特定地理位置的人群中,识别因缺乏HLA-Ⅰ限制性等位基因而不能对表位疫苗产生免疫应答的个体.
목적건립HLA-Ⅰ적루표형빈솔(CPF)적공간예측계통,지재지도중국역묘학가설계수HLA한제적다표위역묘(포괄DNA역묘),병평개혹예측근거불동HLA-Ⅰ조합형식설계적다표위역묘(혹DNA역묘)재중국불동지구적면역효과.방법통과구건중국경내인군HLA-Ⅰ기인공간수거고,이용"극립격"기술건립CPF공간예측계통.결과근거역묘설계목적,설계자가이용해계통예측화고계특정지리위치상인군적CPF,포괄근거단개HLA좌위(HLA-A혹HLA-B)상적등위기인이설계적다표위역묘화근거량개HLA기인좌위(HLA-A화HLA-B)상적등위기인이설계적다표위역묘재특정지리위치상인군적CPF치,종이고계혹예측의연제적혹이연제적다표위역묘재중국불동지리위치상인군중적면역효과.결론해예측계통가용우:①감별임하중국군체적기망CPF백분비,위설계역묘조분제공의거;②재특정지리위치적인군중,예측한제성표위이지적다표위역묘적이론면역응답솔;③재특정지리위치적인군중,식별인결핍HLA-Ⅰ한제성등위기인이불능대표위역묘산생면역응답적개체.
Objective To build the forecasting system on spatial coverage of cumulative phenotypic frequency (CPF) of HLA- Ⅰ for designing HLA-based vaccines (epitopes or DNA vaccines) and evaluating their effects in China. Methods The spatial database of HLA- Ⅰgenes of Chinese (across the land of China) was set up, and then the spatial forecasting system of CPF was designed using Kriging technique.Results Using this spatial forecasting system, the vaccine designer could predict the spatial coverage of CPF for any combination of the alleles confined to a single class Ⅰ locus, either HLA-A or HLA-B, as well as for any combination of alleles at these two loci of each given population at any geographical location across the land of China. Conclusions This system is applicable in the following rields: ①To provide for the identification of alleles that represent a desired percentage of populations across the land of China and that can be targeted for vaccine composition. ② To predict the theoretical responder status of vaccines whose HLA restricted epitopes have already been known in given population at any geographical location across the land of China. (③) To identify how many individuals will be non-responders to a HLA-based vaccine across the land of China.