CAJ | 학술논문

将120只600日龄产蛋山麻鸭随机分为正常日粮组、1/2石粉组和1/4石粉组,自动生化分析仪测定更换饲料前1 d及更换饲料后1、3、5周血清钙、磷水平,观察缺钙日粮对蛋鸭血清钙、磷的影响.分离山麻鸭破骨细胞(OC),在此基础上添加不同浓度钙离子(2、3、5、7 mmol·L~(-1)),倒置显微镜观察体外培养OC形态,培养1、3、5、7 d进行抗酒石酸酸性磷酸酶染色(TRAP),7 d后扫描电镜观察象牙片吸收陷窝.结果表明:钙缺乏可导致蛋鸭血清钙、磷水平升高,降低钙磷比例.高浓度钙和较低浓度钙显著抑制OC生成及骨吸收活性.结果说明缺钙可诱导OC生成并发挥骨吸收功能,最终导致骨代谢性疾病.
장120지600일령산단산마압수궤분위정상일량조、1/2석분조화1/4석분조,자동생화분석의측정경환사료전1 d급경환사료후1、3、5주혈청개、린수평,관찰결개일량대단압혈청개、린적영향.분리산마압파골세포(OC),재차기출상첨가불동농도개리자(2、3、5、7 mmol·L~(-1)),도치현미경관찰체외배양OC형태,배양1、3、5、7 d진행항주석산산성린산매염색(TRAP),7 d후소묘전경관찰상아편흡수함와.결과표명:개결핍가도치단압혈청개、린수평승고,강저개린비례.고농도개화교저농도개현저억제OC생성급골흡수활성.결과설명결개가유도OC생성병발휘골흡수공능,최종도치골대사성질병.
To investigate the effects of serum calcium and phosphorus on the model of calcium-deficient laying ducks, one hundred and twenty Shan sheldrakes were allocated into normal diet, half calcium carbonate and quarter calcium car-bonate groups randomly. Serum calcium and phosphorus were detected by automatic biochemistry analyzer one day before and one, three and five weeks after fed with above diets. Osteoelasts (OC) isolated from Shan sheldrakes were cultured with different concentrations of calcium(2,3,5,7 mmol·L~(-1)).OCs were identified by tartrate resistant acid phosphatase (TRAP) staining and observed by phase-contrast microscope at 1, 3, 5, 7 d after treated by calcium. 7 d later, OC activity was evaluated by assessment of bone slices absorption degree. The results showed that ducks' serum calcium, phosphorus increased, while the proportion of calcium and phosphorus decreased in groups with calcium-deficiency diets. OC formation and activity were inhibited by higher concentrations of calcium. These indicate that calcium deficiency can induce bone metabolic disease by stimulating OC formation and bone resorption.