CAJ | 학술논문

背景:多数学者认为正常乳腺组织中无神经内分泌细胞,乳腺病变后出现神经内分泌细胞是乳腺上皮干细胞分化过程中受局部微环境和激素水平影响所发生的突变、异常分化结果.目的:通过检测人乳腺病变组织中乳腺癌耐药蛋白、细胞角蛋白8及嗜铬蛋白A的表达,从干细胞多向分化的角度探讨人乳腺病变中出现神经内分泌细胞的可能机制.方法:用乳腺癌耐药蛋白作为SP干细胞标记物,用细胞角蛋白8作为腺上皮分化标记物,用嗜铬蛋白A作为神经内分泌分化指标标记物,采用免疫组化方法分别检测这3种蛋白在89例人乳腺组织中的表达情况,并分析3者间的相关性.结果与结论:乳腺癌耐药蛋白与细胞角蛋白8在正常乳腺组织、乳腺增生组织、乳腺病变组织中均有表达,乳腺癌耐药蛋白在病变组织中的表达呈上升趋势,细胞角蛋白8的表达则随乳腺组织异常分化程度的降低呈逐渐减少,嗜铬蛋白A只在乳腺病变组织中有表达.在正常乳腺组织、乳腺增生组织和乳腺病变组织中,乳腺癌耐药蛋白与嗜铬蛋白A的阳性表达呈显著相关(P < 0.01),与细胞角蛋白8的阳性表达无明显相关(P=0.069).上述结果表明正常及增生乳腺组织中未发现神经内分泌细胞,乳腺病变组织中发现神经内分泌细胞,其机制可能与多向分化潜能干细胞的分化有关.
배경:다수학자인위정상유선조직중무신경내분비세포,유선병변후출현신경내분비세포시유선상피간세포분화과정중수국부미배경화격소수평영향소발생적돌변、이상분화결과.목적:통과검측인유선병변조직중유선암내약단백、세포각단백8급기락단백A적표체,종간세포다향분화적각도탐토인유선병변중출현신경내분비세포적가능궤제.방법:용유선암내약단백작위SP간세포표기물,용세포각단백8작위선상피분화표기물,용기락단백A작위신경내분비분화지표표기물,채용면역조화방법분별검측저3충단백재89례인유선조직중적표체정황,병분석3자간적상관성.결과여결론:유선암내약단백여세포각단백8재정상유선조직、유선증생조직、유선병변조직중균유표체,유선암내약단백재병변조직중적표체정상승추세,세포각단백8적표체칙수유선조직이상분화정도적강저정축점감소,기락단백A지재유선병변조직중유표체.재정상유선조직、유선증생조직화유선병변조직중,유선암내약단백여기락단백A적양성표체정현저상관(P < 0.01),여세포각단백8적양성표체무명현상관(P=0.069).상술결과표명정상급증생유선조직중미발현신경내분비세포,유선병변조직중발현신경내분비세포,기궤제가능여다향분화잠능간세포적분화유관.
BACKGROUND: Generally speaking, neuroendocrine cells have been not observed in normal breast tissue but found in breast carcinoma tissue which was affected by local microenvironment and hormone level during differentiation of breast epithelial stem cells.OBJECTIVE: By detecting expressions of breast cancer resistance protein (BCRP), cytokeratin-8 (CK8), and chromogranin-A (CgA) in breast carcinoma tissue, to explore the possible mechanism of neuroendocrine cells observed in breast carcinoma tissue during differentiation of multi-lineage potential of bone marrow mesenchymal stem cells.METHODS: BCRP, CK8, and CgA were used as markers for SP stem cells, glandular epithelium differentiation, and neuroendocrine differentiation, respectively. Immunohistochemistry was used to detect the expressions of BCRP, CK8, and CgA in breast tissues of 89 subjects and analyze their correlation. RESULTS AND CONCLUSION: Both BCRP and CK8 expressions were observed in normal breast tissues, hyperplastic tissues, and breast carcinoma tissue. BCRP expression was increased in the breast carcinoma tissue; CK8 expression was decreased with the abnormal differentiation of breast tissue; CgA expression was only detected in breast carcinoma tissue. BCRP expression was significantly correlated with positive CgA expression (P < 0.01), but it was no correlation with positive CK8 expression in normal breast tissues, hyperplastic tissues, and breast carcinoma tissue (P=0.069). The results suggested that neuroendocrine cells were not observed in both normal breast tissues and hyperplastic tissues but in breast carcinoma tissue, which possibly correlated to differentiation of multi-lineage potential of bone marrow mesenchymal stem cells.