CAJ | 학술논문

目的探讨影响移植肾功能延迟恢复(DGF)的相关因素.方法收集肾移植受者150例临床资料.其中24例发生DGF,对可能影响DGF发生的各项指标进行统计学分析.提取移植肾供者172例和健康体检者157例外周血中基因组DNA,应用多重PCR和特异性引物多态PCR技术检测谷胱甘肽硫转移酶(GST)基因多态性,比较DGF和无DGF组供者GST基因多态性的差异.结果受者DGF组和非DGF组性别(χ~2=0.028,P=0.867)、PRA(χ~2=1.564,P=0.211)及透析类型(χ~2=0.585,P=0.444)之间比较差异无统计学意义(P>0.05);单因素线性回归分析提示术后第2个24 h尿量、第1和第2个24 h入量与T_(1/2(SCr))间存在线性关系(P<0.05),Cox风险比例回归模型提示术后第2个24 h尿量可判断受者移植肾功能的恢复情况(RR=1.002,P=0.001).DGF组供者GSTMl基因型缺失频率为86.4%,与未发生DGF组的62.7%、健康对照组的47.8%比较差异有统计学意义(P<0.05).结论受者术后第2个24 h尿量对预测DGF的发生有重要意义.供者GSTMl基因型缺失可能是发生DGF的原因之一.
목적 탐토영향이식신공능연지회복(DGF)적상관인소.방법 수집신이식수자150례림상자료.기중24례발생DGF,대가능영향DGF발생적각항지표진행통계학분석.제취이식신공자172례화건강체검자157예외주혈중기인조DNA,응용다중PCR화특이성인물다태PCR기술검측곡광감태류전이매(GST)기인다태성,비교DGF화무DGF조공자GST기인다태성적차이.결과 수자DGF조화비DGF조성별(χ~2=0.028,P=0.867)、PRA(χ~2=1.564,P=0.211)급투석류형(χ~2=0.585,P=0.444)지간비교차이무통계학의의(P>0.05);단인소선성회귀분석제시술후제2개24 h뇨량、제1화제2개24 h입량여T_(1/2(SCr))간존재선성관계(P<0.05),Cox풍험비례회귀모형제시술후제2개24 h뇨량가판단수자이식신공능적회복정황(RR=1.002,P=0.001).DGF조공자GSTMl기인형결실빈솔위86.4%,여미발생DGF조적62.7%、건강대조조적47.8%비교차이유통계학의의(P<0.05).결론 수자술후제2개24 h뇨량대예측DGF적발생유중요의의.공자GSTMl기인형결실가능시발생DGF적원인지일.
Objective To explore the factors related to the delayed graft function (DGF). Methods Clinical data of 150 recipients were collected and performed by Cox proportional hazards regression analysis . In addition, the glutathione S-transferase (GST) gene polymorphism of 172 donors and 157 healthy persons was analyzed by multiple PCR and SSP-PCR. Results DGF was observed in 24 patients among 150 recipients. Pretranplantation dialysis mode, PR A levels and recipient gender were uncorrelated with the incidence of DGF(P>0. 05). Urinary volume of the second 24 hours after transplantation was an independent predictor of DGF(RR=1. 002, P = 0. 001). The frequency of donor's null GSTM1 in DGF group was significantly higher than that in non-DGF group(P<0. 05). Conclusions Urinary volume of the second 24 hours after transplantation could be a predictor for DGF. The null GSTM1 in donor might be one of the factors related to the EGF.