CAJ | 학술논문

目的研究民航飞行人员载脂蛋白B基因3'端高可变区串联重复序列(ApoB3'VNTR)多态性及该群体高血脂产生的影响因素.方法聚合酶链反应(PCR)扩增目的基因,琼脂糖凝胶电泳分析PCR产物.血脂测定用常规试剂盒.一般信息采用问卷调查.结果民航飞行人员血脂异常率(49.5%)及体质量指数(BMI)异常率(55.6%)明显高于同年龄段国内男性普通人员(分别为29.2%和49.1%),差异有统计学意义(P<0.01). 2个航空公司682名飞行人员共检测到ApoB3'VNTR 16种等位基因和54种基因型,两地的等位基因和基因型频率分布趋势一致,但与国内普通汉族人比较有明显差异.主要特点为飞行人员纯合子的频率(76.54%)明显高于国内外普通人群(21.50%).血脂异常者大等位基因(VNTR≥39)频率分布高于血脂正常者.协方差分析发现,BMI、低密度脂蛋白(LDL)和总胆同醇水平(TC)均随累计飞行小时的变化而变化,差异有统计学意义(P<0.05).多因素logistic回归分析显示,BMI是唯一影响血脂的因素,而累计飞行小时又是影响BMI的唯一因素.BMI(Y)与累计飞行小时对数(X)的直线回归方程为:(Y)=2.730X+13.584(R~2=0.159,P<0.01).结论民航飞行人员ApoB3'VN-TR多态性可能有特殊的遗传学特点.大等位基因与血脂水平升高有关.飞行负荷可直接影响飞行人员的血脂水平和BMI,也可间接通过BMI影响飞行人员的血脂水平.
목적 연구민항비행인원재지단백B기인3'단고가변구천련중복서렬(ApoB3'VNTR)다태성급해군체고혈지산생적영향인소.방법 취합매련반응(PCR)확증목적 기인,경지당응효전영분석PCR산물.혈지측정용상규시제합.일반신식채용문권조사.결과 민항비행인원혈지이상솔(49.5%)급체질량지수(BMI)이상솔(55.6%)명현고우동년령단국내남성보통인원(분별위29.2%화49.1%),차이유통계학의의(P<0.01). 2개항공공사682명비행인원공검측도ApoB3'VNTR 16충등위기인화54충기인형,량지적등위기인화기인형빈솔분포추세일치,단여국내보통한족인비교유명현차이.주요특점위비행인원순합자적빈솔(76.54%)명현고우국내외보통인군(21.50%).혈지이상자대등위기인(VNTR≥39)빈솔분포고우혈지정상자.협방차분석발현,BMI、저밀도지단백(LDL)화총담동순수평(TC)균수루계비행소시적변화이변화,차이유통계학의의(P<0.05).다인소logistic회귀분석현시,BMI시유일영향혈지적인소,이루계비행소시우시영향BMI적유일인소.BMI(Y)여루계비행소시대수(X)적직선회귀방정위:(Y)=2.730X+13.584(R~2=0.159,P<0.01).결론 민항비행인원ApoB3'VN-TR다태성가능유특수적유전학특점.대등위기인여혈지수평승고유관.비행부하가직접영향비행인원적혈지수평화BMI,야가간접통과BMI영향비행인원적혈지수평.
Objective To study the frequency distribution in polymorphism of the apoprotein B 3' variable number tandem repeat (ApoB3~'VNTR) and influence factors on hyperlipemia in civil aircrew.Methods ApoB genetypes were determined by PCR technology and agarose gel electrophoresis. The blood lipids were measured by routine kits. Personal information of flight personnel was collected by questionnaire.Results Prevalence of the total dyslipidemia (49.5%) and overweight (55.6%) of flight personnel were much higher than that of domestic general population (29.2% and 49.1%) respectively (P<0.05). There were 16 al-leles and 54 kinds of genotypes of ApoB3~' VNTR in the 682 flight personnel. The frequency distribution of al-leles and genotypes of aircrew in the two air companies had same trend, which was different from the general population. The frequency of the homozygote was 76.54%, which was much higher than that of the other peoples home and abroad (21.50%).The frequency of the big allele (VNTR=39) in hyperlipemia groups were higher than that of normal groups. By analysis of co-variance, the body mass index (BMI), low density lipoprotein (LDL) and the total cholesterol (TC) increased with the cumulate flight hours (P<0.05). In multi-variate logistic regression analysis, the BMI was the only factor influencing blood lipids, and the cumulate flight hours was only factors affecting the BMI. Taking the cumulate flight hours logarithm as the independent variable (X), and the BMI as dependent variable(Y), the linearity equations was: (Y)=2.730X+13.584(R~2=0.159, P<0.01).Conclusion There are perhaps special genetic characteristics in the polymorphism of the ApoB3' VNTR in the aircrew. The big allele is correlated with the hyperlipemia. The flight burden not only directly affects the BMI and blood lipids levels, but also it can indirectly affect the lipids levels by BMI.