CAJ | 학술논문

目的建立并初步评价多元检测引起病毒性出血热病原体特异性IgM抗体的方法.方法在原核细胞中重组表达纯化马尔堡病毒、拉沙热病毒、裂谷热病毒、肺综合征出血热汉坦病毒、汉滩病毒、Seoul病毒及普马拉病毒核蛋白(NP),共价偶联到7种不同的xMAP荧光微球上.优化评价偶联效果,通过参比血清中相应病毒NP特异性抗体检测,评估检测方法,并与常规使用的MacELISA方法进行比较.结果在Luminex平台的基础上建立了多元检测引起病毒性出血热的病毒核蛋白特异性抗体的方法,特异性与敏感性与常规应用的特异性抗体检测MacELISA试剂盒相当,但可以同时排查多个病毒感染情况.结论利用Luminex xMAP技术建立基于病毒核蛋白多元检测方法快速敏感,操作简单,可以用于病毒性出血热的检测和血清流行病学调查.
목적 건립병초보평개다원검측인기병독성출혈열병원체특이성IgM항체적방법.방법 재원핵세포중중조표체순화마이보병독、랍사열병독、렬곡열병독、폐종합정출혈열한탄병독、한탄병독、Seoul병독급보마랍병독핵단백(NP),공개우련도7충불동적xMAP형광미구상.우화평개우련효과,통과삼비혈청중상응병독NP특이성항체검측,평고검측방법,병여상규사용적MacELISA방법진행비교.결과 재Luminex평태적기출상건립료다원검측인기병독성출혈열적병독핵단백특이성항체적방법,특이성여민감성여상규응용적특이성항체검측MacELISA시제합상당,단가이동시배사다개병독감염정황.결론 이용Luminex xMAP기술건립기우병독핵단백다원검측방법쾌속민감,조작간단,가이용우병독성출혈열적검측화혈청류행병학조사.
Objective To develop and evaluate a multiplex detection of IgM antibodies to pathogens caused viral hemorrhagic fever. Methods The nucleocapsid proteins (NP) of HTN, SEO, Puu MBV, Lassa, RFV and HPS viruses expressed in prokaryotic cells and purified were coupled to 7 different xMAP fluorescent microbeads. The assay was evaluated and optimized when screened against a panel of reference sera collected from HFRS patients, and compared to commonly used MacELISA Kits. Results For detection of anti-NP antibodies, the sensitivity and specificity of the assay were comparable to a commonly used MacELISA kit, but it could detect different antigen specific antibodies in one reaction simultaneously. Conclusion A robust, rapid and multiplex assay based on NPs could be developed via Luminex xMAP platform for laboratory diagnosis of viral hemorrhagic fever and seroepidemiological investigation.