CAJ | 학술논문

目的观察Polo样激酶1(PLK1)在原发性肝癌细胞中的表达,探讨其与肝癌患者临床病理特点及预后的关系.方法采用免疫组织化学技术观察PLK1在40例原发性肝癌组织石蜡切片中的表达.结果　癌栓、包膜侵犯、BCLC分期等因素和PLK1的阳性表达呈明显相关(P＜0.05);PLK1阳性组和阴性组的术前甲胎蛋白(AFP)差异有统计学意义(P＜0.05);秩和检验分析PLK1表达与患者肿瘤直径大小的关系发现PLK1阳性组和阴性组之间差异有统计学意义(P＜0.01).生存分析显示,PLK1的表达和患者的生存期无明显相关;Kaplan-Meier法分析原发性肝癌的预后的单因素相关因子显示与包膜侵犯、TNM分期、癌栓及转移与生存期明显相关(P＜0.05),与性别、乙肝、肝硬化、肿瘤数目BCLC分期及临床分期无明显相关;多因素Cox回归分析显示包膜侵犯、癌栓及转移3项指标反映肝癌预后(P＜0.05).结论 PLK1的表达与肝癌的发展及其临床生物学行为密切相关.
목적 관찰Polo양격매1(PLK1)재원발성간암세포중적표체,탐토기여간암환자림상병리특점급예후적관계.방법 채용면역조직화학기술관찰PLK1재40례원발성간암조직석사절편중적표체.결과　암전、포막침범、BCLC분기등인소화PLK1적양성표체정명현상관(P＜0.05);PLK1양성조화음성조적술전갑태단백(AFP)차이유통계학의의(P＜0.05);질화검험분석PLK1표체여환자종류직경대소적관계발현PLK1양성조화음성조지간차이유통계학의의(P＜0.01).생존분석현시,PLK1적표체화환자적생존기무명현상관;Kaplan-Meier법분석원발성간암적예후적단인소상관인자현시여포막침범、TNM분기、암전급전이여생존기명현상관(P＜0.05),여성별、을간、간경화、종류수목BCLC분기급림상분기무명현상관;다인소Cox회귀분석현시포막침범、암전급전이3항지표반영간암예후(P＜0.05).결론 PLK1적표체여간암적발전급기림상생물학행위밀절상관.
Objective To investigate the expression of Polo-like kinase 1 (PLK1) in patients with hepatocellular cancer (HCC), and find out its correlation with clinicopathological features and prognosis of HCC. Methods The expression of PLK1 in 40 patients with primary HCC was detected by using immunohistochemistry (IHC) and analyzed with SPSS13.0 software. Results Significant positive correlations were revealed between PLK1 expression and tumor thrombosis, envelope evasion and BCLC staging ( P ＜0. 05). Meanwhile, there were significant differences in preoperative alpha fetal protein (AFP) level and tumor diameter between PLK1 positive and negative groups ( P ＜ 0. 05). However, no significant correlation was observed between PLK1 expression and survival time of HCC. Factors, including envelope evasion, TNM staging, tumor thrombosis and metastasis, were significantly correlated with the survival time ( P ＜ 0. 05), while Cox regression analysis showed that envelope evasion, tumor thrombosis and metastasis were significantly correlated with HCC prognosis (P ＜ 0. 05). Conclusion PLK1 is closely correlated with the development and clinicobiological characteristics of HCC.