CAJ | 학술논문

目的: 研究凤尾草抑制前列腺上皮细胞增生的活性化学成分.方法:大孔树脂、凝胶、正相及反相硅胶柱层析方法用于化合物的分离;红外、紫外、核磁和质谱等方法用于鉴定化合物结构.化合物对大鼠前列腺上皮细胞的抑制活性用MT T方法测定.结果:从凤尾草的丙酮和20%乙醇提取物中,分离并鉴定了22个化合物,其中化合物木犀草素,棕榈酸和芹菜素4′-O-α-L-鼠李糖苷显示与爱普列特相比拟的大鼠前列腺上皮细胞增生体外抑制效应.结论: 化合物pteroside P′ (1)是一个新的C14的倍半萜苷;化合物木犀草素、棕榈酸和芹菜素4′-O-α-L-鼠李糖苷可能是凤尾草能抑制良性前列腺增生的主要活性成分.
목적: 연구봉미초억제전렬선상피세포증생적활성화학성분.방법:대공수지、응효、정상급반상규효주층석방법용우화합물적분리;홍외、자외、핵자화질보등방법용우감정화합물결구.화합물대대서전렬선상피세포적억제활성용MT T방법측정.결과:종봉미초적병동화20%을순제취물중,분리병감정료22개화합물,기중화합물목서초소,종려산화근채소4′-O-α-L-서리당감현시여애보렬특상비의적대서전렬선상피세포증생체외억제효응.결론: 화합물pteroside P′ (1)시일개신적C14적배반첩감;화합물목서초소、종려산화근채소4′-O-α-L-서리당감가능시봉미초능억제량성전렬선증생적주요활성성분.
AIM: To study the active chemical constituents of Pteris multifida against growth of rat prostatic epithelial cells (PECs). METHODS: Column chromatographies of macroporous resin D-1400, Sephadex LH-20, Rp-18, silica gel were used for the isolation of compounds, and spectroscopic techniques (NMR, IR, UV and MS) were used for the structural identification . The inhibitory activities of compounds on rat PECs growth were evaluated by MTT method under serum-free conditions with or without growth factors added to the culture medium. RESULTS: 22 compounds were isolated from acetone and 20% ethanol extracts of the titled plant. Among them, luteolin, palmitic acid and apigenin 4′-O-α-L-rhamnopyranoside showed close effect against growth of rat PECs in vitro with epristeride. CONCLUSION: Pteroside P′(1) was identified to be a new C14 sesquiterpene glycoside; luteolin, palmitic acid and apigenin 4′-O-α-L-rhamnopyranoside may be the main active components of Pteris multifida against benign prostatic hypertrophy.