CAJ | 학술논문

目的研究手术创伤动员荷瘤裸鼠血管内皮祖细胞(EPC)入血与实体瘤生长的关系.方法将42只荷瘤裸鼠随机分为非手术1 d组、单纯麻醉组、手术创伤组(术后24 h、48 h、72 h、30 d组)及非手术30 d组,每组6只.单纯麻醉组24 h后取血及获取移植瘤组织,手术创伤组分别在术后24 h、48 h、72 h、30 d取血及获取移植瘤组织.流式细胞仪检测各组外周血EPC百分比;ELISA检测血清血管内皮生长因子(VEGF)水平;免疫组织化学法检测移植瘤组织VEGF表达及微血管密度(MVD).结果术后24 h、48 h、72 h组EPC百分比与非手术1 d组比较,差异均有统计学意义(P<0.05);术后24 h组、48 h组、72 h组、单纯麻醉组VEGF水平与非手术1 d组比较,差异有统计学意义(P<0.05);各组MVD差异无统计学意义(P>0.05).Pearson相关性分析显示,血清VEGF水平与外周血EPC百分比呈正相关(r=0.695 6,P<0.01),外周血EPC百分比与MVD无相关性(r=0.221 4,P>0.05),血清VEGF水平与MVD也无相关性(r=0.224 9,P>0.05).结论手术创伤对移植瘤生长无明显促进作用.
목적 연구수술창상동원하류라서혈관내피조세포(EPC)입혈여실체류생장적관계.방법 장42지하류라서수궤분위비수술1 d조、단순마취조、수술창상조(술후24 h、48 h、72 h、30 d조)급비수술30 d조,매조6지.단순마취조24 h후취혈급획취이식류조직,수술창상조분별재술후24 h、48 h、72 h、30 d취혈급획취이식류조직.류식세포의검측각조외주혈EPC백분비;ELISA검측혈청혈관내피생장인자(VEGF)수평;면역조직화학법검측이식류조직VEGF표체급미혈관밀도(MVD).결과 술후24 h、48 h、72 h조EPC백분비여비수술1 d조비교,차이균유통계학의의(P<0.05);술후24 h조、48 h조、72 h조、단순마취조VEGF수평여비수술1 d조비교,차이유통계학의의(P<0.05);각조MVD차이무통계학의의(P>0.05).Pearson상관성분석현시,혈청VEGF수평여외주혈EPC백분비정정상관(r=0.695 6,P<0.01),외주혈EPC백분비여MVD무상관성(r=0.221 4,P>0.05),혈청VEGF수평여MVD야무상관성(r=0.224 9,P>0.05).결론 수술창상대이식류생장무명현촉진작용.
Objective To explore the correlation of tumor growth and endothelial progenitor cells (EPC) entering blood induced by surgical injury in tumor bearing nude mice. Methods Forty-two tumor bearing nude mice were randomly divided into seven groups (n=6): non-surgical injury groups (1 d and 30 d), anesthetic group, surgical injury groups (24 h, 48 h, 72 h and 30 d after surgery). Blood samples and xenograft tumor tissues were taken from anesthetic group 24 h after anaesthesia and surgical injury groups 24 h, 48 h, 72 h and 30 d after surgery. EPC levels in peripheral blood were measured by flow cytometry, serum VEGF levels were determined by ELISA, microvessel density (MVD) and expression of VEGF were detected by immunohistochemistry. Results The levels of EPC in 24 h post-surgery group, 48 h post-surgery group and 72 h post-surgery group were significantly higher than that in non-surgical injury 1 d group (P<0.05). The levels of VEGF in 24 h post-surgery group, 48 h post-surgery group, 72 h post-surgery group and anesthetic group were significantly higher than that in non-surgical injury 1 d group (P<0.05). There was no significant difference in MVD among groups (P>0.05). Pearson correlation analysis revealed that serum VEGF levels were related to EPC levels in peripheral blood (r=0.695 6, P<0.01), while EPC levels in peripheral blood were not related to MVD (r=0.221 4, P>0.05), and serum VEGF levels had no correlation with MVD (r=0.224 9, P>0.05). Conclusion Surgical injury has no obvious influence on xenograft tumor growth.