分析测试学报
分析測試學報
분석측시학보
JOURNAL OF INSTRUMENTAL ANALYSIS
2010年
1期
51-54
,共4页
荧光%脱氧核酶8-17%探针%铅离子
熒光%脫氧覈酶8-17%探針%鉛離子
형광%탈양핵매8-17%탐침%연리자
fluorescence%8-17 DNAzyme%probe%lead ion
将8-17 DNAzyme增加2个"G-C"碱基对进行增强热稳定性的结构修饰,并标记上1个荧光基团"FAM"和2个荧光猝灭基团"Dabcyl",设计成双猝灭Pb~(2+)荧光探针.研究了该探针对Cd~(2+)、Zn~(2+)、Mg~(2+)、Cu~(2+)、Mn~(2+)、Pb~(2+) 6种二价金属离子的响应,结果表明探针对Pb~(2+)具有很强的特异性,在探针浓度为2.5×10~(-7) mol/L时,Pb~(2+)浓度在8.5×10~(-8) ～ 7.5×10~(-6) mol/L范围内和探针的荧光强度呈线性关系,检出限为8.5×10~(-8) mol/L.该探针可用于Pb~(2+)的定性和定量检测.
將8-17 DNAzyme增加2箇"G-C"堿基對進行增彊熱穩定性的結構脩飾,併標記上1箇熒光基糰"FAM"和2箇熒光猝滅基糰"Dabcyl",設計成雙猝滅Pb~(2+)熒光探針.研究瞭該探針對Cd~(2+)、Zn~(2+)、Mg~(2+)、Cu~(2+)、Mn~(2+)、Pb~(2+) 6種二價金屬離子的響應,結果錶明探針對Pb~(2+)具有很彊的特異性,在探針濃度為2.5×10~(-7) mol/L時,Pb~(2+)濃度在8.5×10~(-8) ～ 7.5×10~(-6) mol/L範圍內和探針的熒光彊度呈線性關繫,檢齣限為8.5×10~(-8) mol/L.該探針可用于Pb~(2+)的定性和定量檢測.
장8-17 DNAzyme증가2개"G-C"감기대진행증강열은정성적결구수식,병표기상1개형광기단"FAM"화2개형광졸멸기단"Dabcyl",설계성쌍졸멸Pb~(2+)형광탐침.연구료해탐침대Cd~(2+)、Zn~(2+)、Mg~(2+)、Cu~(2+)、Mn~(2+)、Pb~(2+) 6충이개금속리자적향응,결과표명탐침대Pb~(2+)구유흔강적특이성,재탐침농도위2.5×10~(-7) mol/L시,Pb~(2+)농도재8.5×10~(-8) ～ 7.5×10~(-6) mol/L범위내화탐침적형광강도정선성관계,검출한위8.5×10~(-8) mol/L.해탐침가용우Pb~(2+)적정성화정량검측.
Structure of 8-17 DNAzyme was modified with two pairs of "G-C"to enhance its secondary structure thermal stability and a double quenching fluorescent probe was designed by employing one fluorophore "FAM" and two quenchers "Dabcyl" based on the fluorescence resonance energy transfer(FRET) principle. The qualitative assays was studied by monitoring the change of the probe fluorescent intensity after adding Cd~(2+),Zn~(2+),Mg~(2+),Cu~(2+),Mn~(2+),Pb~(2+),individually.Among these metal ions,Pb~(2+) showed a strong specificity to the probe.When the probe concentration was 2.5×10~(-7) mol/L,a good linear relationship was found between the fluorescence intensities and Pb~(2+) concentration in the range of 8.5×10~(-8)-7.5×10~(-6) mol/L with a detection limit of 8.5×10~(-8) mol/L.The results showed that the probe could be used for both qualitative and quantitative detection of Pb~(2+) at room temperature.
