CAJ | 학술논문

为了寻找与小麦雌性育性紧密关联的SSR分子标记,并估算其表型效应,选择均匀分布于小麦基因的63个SSR标记,对包括小麦雌性不育系XND126在内的261个品种(系)组成的群体进行分析,并使用STRUCTRE软件进行群体结构评估,利用TASSEL软件的GLM模型检测多态性标记与小麦雌性育性(国内法育性D.F.和国际法育性I.F.)两种表型值的关联程度.结果表明:目标群体中存在3个亚群.基因组中多态性标记关联分析初步显示,标记Xcfd36(2AS、2DS)和Xcfd88(4AL)与小麦雌性育性两种表型值相关联,两个标记对D.F.和I.F.的效应分别是0.1585,0.113和0.087,0.0596.进一步分析发现2DS和4AL连锁群上存在一些与小麦雌性育件更加紧密关联的标记,如Xwmc25(2DS)和Xwmc232(4AL),其效应分别可达到0.5833和0.2841,而2AS上未找到独有的关联标记.分析认为小麦染色体2DS和4AL各存在一个控制雌性育性的基因位点,其中2DS上的QTL被连锁分析所证实.对与表型紧密关联的标记的鉴别对于小麦雌性育性QTL发掘的重要作用进行了探讨.
위료심조여소맥자성육성긴밀관련적SSR분자표기,병고산기표형효응,선택균균분포우소맥기인적63개SSR표기,대포괄소맥자성불육계XND126재내적261개품충(계)조성적군체진행분석,병사용STRUCTRE연건진행군체결구평고,이용TASSEL연건적GLM모형검측다태성표기여소맥자성육성(국내법육성D.F.화국제법육성I.F.)량충표형치적관련정도.결과표명:목표군체중존재3개아군.기인조중다태성표기관련분석초보현시,표기Xcfd36(2AS、2DS)화Xcfd88(4AL)여소맥자성육성량충표형치상관련,량개표기대D.F.화I.F.적효응분별시0.1585,0.113화0.087,0.0596.진일보분석발현2DS화4AL련쇄군상존재일사여소맥자성육건경가긴밀관련적표기,여Xwmc25(2DS)화Xwmc232(4AL),기효응분별가체도0.5833화0.2841,이2AS상미조도독유적관련표기.분석인위소맥염색체2DS화4AL각존재일개공제자성육성적기인위점,기중2DS상적QTL피련쇄분석소증실.대여표형긴밀관련적표기적감별대우소맥자성육성QTL발굴적중요작용진행료탐토.
The aims of this study were to identify the SSR markers closely associated with the female fertility, and estimate their genetic effects on female fertilities in wheat. The software STRUCTRE was used to evaluate the structure of the population with 261 wheat varieties and the advanced lines, including a female sterile line XND126, based on the test of 63 SSR markers distributed evenly in wheat genome. The association was analyzed between polymorphic markers and two sets of female fertility phenotypie values (domestic fertility-D.F, and international fertility-I.F.) through the GLM model with the software TASSEL. The results showed that there were three sub-populations in the surveyed population. The candidate markers Xcfd36 (2AS, 2DS) and Xcfd88 (4AL) were associated with the phenotypes in preliminary genome scanning, and the genetic effects of the two markers on D.F. and I.F., were 0.1585, 0.113 and 0.087, 0.0596, respectively. It was also found that some markers such as Xwmc25(2DS) and Xwmc232(4AL)on the candidate markers linkage groups were more closely associated with female fertility in further analysis than those in preliminary scanning, and the genetic effects of the two markers were 0.5833 and 0.2841, respectively, whereas no significantly associated marker was found on 2AS. h is believed that two loci associated with female fertility are located on 2DS and 4AL in wheat genome, and one of the loci on 2DS is validated by QTL mapping in linkage analysis. The screening of the markers closely associated with the female fertility is important for QTLs usage of the female fertility in wheat.