CAJ | 학술논문

GDP-甘露糖焦磷酸化酶(GMPase,EC 2.7.7.22)是维生素C合成途径的第一步关键酶.通过RT-PCR扩增到1498 bp的GMPase全长序列,GenBank登录号为DQ449030.利用克隆到的基因分别构建得到正义及反义植物表达载体.并将其克隆至PBI121真核表达载体,转化农杆菌LBA4404,利用农杆菌介导法转化烟草植株,经基因组PCR及琼脂糖凝胶电泳检测, 结果表明GMPase真核表达载体构建成功,并成功获得转基因植株.
GDP-감로당초린산화매(GMPase,EC 2.7.7.22)시유생소C합성도경적제일보관건매.통과RT-PCR확증도1498 bp적GMPase전장서렬,GenBank등록호위DQ449030.이용극륭도적기인분별구건득도정의급반의식물표체재체.병장기극륭지PBI121진핵표체재체,전화농간균LBA4404,이용농간균개도법전화연초식주,경기인조PCR급경지당응효전영검측, 결과표명GMPase진핵표체재체구건성공,병성공획득전기인식주.
GDP-mannose pyrophosphorylase(GMPase,EC 2.7.7.22)can catalyse the synthesis of GDP-D-mannose and represents the first committed step in the formation of all guanosin-containing sugar nucleotides found in plants which are precursors for the synthesis of L-ascorbate.A full-length cDNA encoding GMPase from Lycopersicon esculentum was isolated using a RT-PCR.Transgenic tobacco plants were generated in which the fragments of tomato GDP-mannose pyrophosphorylase gene(GMPase)cDNA was introduced in antisense orientation to the 35 S promoter.