国际医药卫生导报
國際醫藥衛生導報
국제의약위생도보
INTERNATIONAL MEDICINE & HEALTH GUIDANCE NEWS
2012年
7期
896-899
,共4页
黄卓敏%王楚莹%古衍%姚吉龙%谢建生
黃卓敏%王楚瑩%古衍%姚吉龍%謝建生
황탁민%왕초형%고연%요길룡%사건생
人乳头瘤病毒18%E2%E6%宫颈上皮内瘤变%宫颈癌
人乳頭瘤病毒18%E2%E6%宮頸上皮內瘤變%宮頸癌
인유두류병독18%E2%E6%궁경상피내류변%궁경암
HPV-18%E2%E6%Cervical intraepithelial neoplasia%Cervical carcinoma
目的 探讨HPV-18病毒E2与E6基因在宫颈病变组织中的表达及其在宫颈癌变过程中的作用.方法 采用逆转录聚合酶链反应技术,检测HPV-18阳性的宫颈炎15例、CINⅠ 19例、CIN Ⅱ 17例、CIN Ⅲ 20例、宫颈浸润癌24例的标本中E2及E6基因的表达,比较两者在各组中的表达差异.结果HPV-18 E2在宫颈炎、CIN Ⅰ、CINⅡ、CIN Ⅲ和宫颈浸润癌的阳性表达率分别为93.3%、94.7%、58.8%、20.0%及0%; E6在上述各组的阳性表达率分别为6.7%、5.3%、41.2%、85.0%及100%; E2和E6在宫颈炎和CIN Ⅰ的表达差异无显著性(P>0.05);E2和E6的表达在CIN Ⅰ、CINⅡ、CIN Ⅲ和宫颈浸润癌组间的比较,差异有显著意义(P<0.05);E6和E2的表达呈负相关(r=- 0.728,P<0.01).结论 HPV-18 E2的表达缺失和E6的过度表达,可能是H PV诱导宫颈细胞恶性转化的关键环节,在宫颈癌的演变过程中起重要作用.检测HPV- 18 E2与E6的表达对早期评估CIN的预后可能有一定的参考价值.
目的 探討HPV-18病毒E2與E6基因在宮頸病變組織中的錶達及其在宮頸癌變過程中的作用.方法 採用逆轉錄聚閤酶鏈反應技術,檢測HPV-18暘性的宮頸炎15例、CINⅠ 19例、CIN Ⅱ 17例、CIN Ⅲ 20例、宮頸浸潤癌24例的標本中E2及E6基因的錶達,比較兩者在各組中的錶達差異.結果HPV-18 E2在宮頸炎、CIN Ⅰ、CINⅡ、CIN Ⅲ和宮頸浸潤癌的暘性錶達率分彆為93.3%、94.7%、58.8%、20.0%及0%; E6在上述各組的暘性錶達率分彆為6.7%、5.3%、41.2%、85.0%及100%; E2和E6在宮頸炎和CIN Ⅰ的錶達差異無顯著性(P>0.05);E2和E6的錶達在CIN Ⅰ、CINⅡ、CIN Ⅲ和宮頸浸潤癌組間的比較,差異有顯著意義(P<0.05);E6和E2的錶達呈負相關(r=- 0.728,P<0.01).結論 HPV-18 E2的錶達缺失和E6的過度錶達,可能是H PV誘導宮頸細胞噁性轉化的關鍵環節,在宮頸癌的縯變過程中起重要作用.檢測HPV- 18 E2與E6的錶達對早期評估CIN的預後可能有一定的參攷價值.
목적 탐토HPV-18병독E2여E6기인재궁경병변조직중적표체급기재궁경암변과정중적작용.방법 채용역전록취합매련반응기술,검측HPV-18양성적궁경염15례、CINⅠ 19례、CIN Ⅱ 17례、CIN Ⅲ 20례、궁경침윤암24례적표본중E2급E6기인적표체,비교량자재각조중적표체차이.결과HPV-18 E2재궁경염、CIN Ⅰ、CINⅡ、CIN Ⅲ화궁경침윤암적양성표체솔분별위93.3%、94.7%、58.8%、20.0%급0%; E6재상술각조적양성표체솔분별위6.7%、5.3%、41.2%、85.0%급100%; E2화E6재궁경염화CIN Ⅰ적표체차이무현저성(P>0.05);E2화E6적표체재CIN Ⅰ、CINⅡ、CIN Ⅲ화궁경침윤암조간적비교,차이유현저의의(P<0.05);E6화E2적표체정부상관(r=- 0.728,P<0.01).결론 HPV-18 E2적표체결실화E6적과도표체,가능시H PV유도궁경세포악성전화적관건배절,재궁경암적연변과정중기중요작용.검측HPV- 18 E2여E6적표체대조기평고CIN적예후가능유일정적삼고개치.
Objective To investigate the expressions of human papillomavirus-18 E2 and E6 oncogene in cervical lesions and its correlation with cervical carcinogenesis.Methods The expressions of HPV- 18 E2 and E6 gene in the tissues from 15 cases of cervicitis,19 cases of CIN Ⅰ,17 cases of CIN Ⅱ,20 cases of CIN Ⅲ,and 24 cases of invasion cervical carcinoma with HPV-18 infection were detected by reverse transcription polymerase chain reaction.Results The positive rate of E2 gene expression in groups of cervicitis,CIN Ⅰ,CIN Ⅱ,CIN Ⅲ and invasion cervical carcinoma were 93.3 %,94.7 %,58.8%,20.0% and 0% respectively,and thatofE6genewere6.7%,5.3%,41.2%,85.0%and 100%respectively.No significant differences of E2 and E6 gene expressions were found between groups of cervicitis and CIN Ⅰ ( P> 0.05 ).However,there were significant differences between any two groups of CIN Ⅰ,CIN Ⅱ,CIN Ⅲ and invasion cervical carcinoma ( P< 0.05 ).The expression of E6 gene was inversely correlated with expression of E2 gene ( r=-0.728, P < 0.01 ).Conclusions Deletion of HPV-18 E2 and overexpression of E6 which has been regarded as a key step in malignant transformation of cervical cells inducted by HPV may play an important role in cervical carcinogenesis.Detection of levels of HPV-18 E2 and E6 expression might be clinically valuable in the prediction of cervical carcinogenesis and the evaluation of prognosis in CIN.