CAJ | 학술논문

目的探讨HPV-18病毒E2与E6基因在宫颈病变组织中的表达及其在宫颈癌变过程中的作用.方法采用逆转录聚合酶链反应技术,检测HPV-18阳性的宫颈炎15例、CINⅠ 19例、CIN Ⅱ 17例、CIN Ⅲ 20例、宫颈浸润癌24例的标本中E2及E6基因的表达,比较两者在各组中的表达差异.结果HPV-18 E2在宫颈炎、CIN Ⅰ、CINⅡ、CIN Ⅲ和宫颈浸润癌的阳性表达率分别为93.3％、94.7％、58.8％、20.0％及0％; E6在上述各组的阳性表达率分别为6.7％、5.3％、41.2％、85.0％及100％; E2和E6在宫颈炎和CIN Ⅰ的表达差异无显著性(P＞0.05)；E2和E6的表达在CIN Ⅰ、CINⅡ、CIN Ⅲ和宫颈浸润癌组间的比较,差异有显著意义(P＜0.05)；E6和E2的表达呈负相关(r=- 0.728,P＜0.01).结论 HPV-18 E2的表达缺失和E6的过度表达,可能是H PV诱导宫颈细胞恶性转化的关键环节,在宫颈癌的演变过程中起重要作用.检测HPV- 18 E2与E6的表达对早期评估CIN的预后可能有一定的参考价值.
목적 탐토HPV-18병독E2여E6기인재궁경병변조직중적표체급기재궁경암변과정중적작용.방법 채용역전록취합매련반응기술,검측HPV-18양성적궁경염15례、CINⅠ 19례、CIN Ⅱ 17례、CIN Ⅲ 20례、궁경침윤암24례적표본중E2급E6기인적표체,비교량자재각조중적표체차이.결과HPV-18 E2재궁경염、CIN Ⅰ、CINⅡ、CIN Ⅲ화궁경침윤암적양성표체솔분별위93.3％、94.7％、58.8％、20.0％급0％; E6재상술각조적양성표체솔분별위6.7％、5.3％、41.2％、85.0％급100％; E2화E6재궁경염화CIN Ⅰ적표체차이무현저성(P＞0.05)；E2화E6적표체재CIN Ⅰ、CINⅡ、CIN Ⅲ화궁경침윤암조간적비교,차이유현저의의(P＜0.05)；E6화E2적표체정부상관(r=- 0.728,P＜0.01).결론 HPV-18 E2적표체결실화E6적과도표체,가능시H PV유도궁경세포악성전화적관건배절,재궁경암적연변과정중기중요작용.검측HPV- 18 E2여E6적표체대조기평고CIN적예후가능유일정적삼고개치.
Objective To investigate the expressions of human papillomavirus-18 E2 and E6 oncogene in cervical lesions and its correlation with cervical carcinogenesis.Methods The expressions of HPV- 18 E2 and E6 gene in the tissues from 15 cases of cervicitis,19 cases of CIN Ⅰ,17 cases of CIN Ⅱ,20 cases of CIN Ⅲ,and 24 cases of invasion cervical carcinoma with HPV-18 infection were detected by reverse transcription polymerase chain reaction.Results The positive rate of E2 gene expression in groups of cervicitis,CIN Ⅰ,CIN Ⅱ,CIN Ⅲ and invasion cervical carcinoma were 93.3 ％,94.7 ％,58.8％,20.0％ and 0％ respectively,and thatofE6genewere6.7％,5.3％,41.2％,85.0％and 100％respectively.No significant differences of E2 and E6 gene expressions were found between groups of cervicitis and CIN Ⅰ ( P＞ 0.05 ).However,there were significant differences between any two groups of CIN Ⅰ,CIN Ⅱ,CIN Ⅲ and invasion cervical carcinoma ( P＜ 0.05 ).The expression of E6 gene was inversely correlated with expression of E2 gene ( r=-0.728, P ＜ 0.01 ).Conclusions Deletion of HPV-18 E2 and overexpression of E6 which has been regarded as a key step in malignant transformation of cervical cells inducted by HPV may play an important role in cervical carcinogenesis.Detection of levels of HPV-18 E2 and E6 expression might be clinically valuable in the prediction of cervical carcinogenesis and the evaluation of prognosis in CIN.