CAJ | 학술논문

目的探讨孕期应激对子代雌鼠成年后酒精摄取行为以及脑内小白蛋白表达的影响.方法 6只SD孕鼠随机分为孕期应激组(PS组,n=3)和对照组(CON组,n=3).PS组孕鼠在妊娠晚期接受限制性应激,CON组孕鼠不给予孕期应激.子代成年后,PS组和CON组雌性子代分别随机分为PS-S组(n=6)和PS-NS组(n=6),CON-S组(n=6)和CON-NS组(n=6).PS-S组和CON-S组动物先后接受限制性应激和冰水应激.所有动物均给予水和酒精溶液进行喂养,比较各组子鼠的基础酒精摄人量和子代应激后的酒精摄入量.用免疫组化方法检测子代海马和前额皮质小白蛋白的表达.结果 PS-NS组在第1,4,6周的酒精摄入量明显低于CON-NS组(P<0.05).PS-S组在限制性应激后第1周的酒精摄入量[(3.79±0.47)kg·d-1·g-1]明显高于CON-S组[(1.68±0.68)kg·d-1·g-1](P<0.05),而PS-S组动物在冰水应激后第3周的酒精摄入明显小于CON-S组(P<0.05).另外,PS-S组子代海马CA2区和前额皮质M1区的小白蛋白表达均明显低于CON-S组(15.82±0.30,26.52±0.12,P<0.05).结论孕期应激可影响成年雌性子代的酒精摄取行为,此过程可能与海马和前额皮质小白蛋白表达的减少有关.
목적 탐토잉기응격대자대자서성년후주정섭취행위이급뇌내소백단백표체적영향.방법 6지SD잉서수궤분위잉기응격조(PS조,n=3)화대조조(CON조,n=3).PS조잉서재임신만기접수한제성응격,CON조잉서불급여잉기응격.자대성년후,PS조화CON조자성자대분별수궤분위PS-S조(n=6)화PS-NS조(n=6),CON-S조(n=6)화CON-NS조(n=6).PS-S조화CON-S조동물선후접수한제성응격화빙수응격.소유동물균급여수화주정용액진행위양,비교각조자서적기출주정섭인량화자대응격후적주정섭입량.용면역조화방법검측자대해마화전액피질소백단백적표체.결과 PS-NS조재제1,4,6주적주정섭입량명현저우CON-NS조(P<0.05).PS-S조재한제성응격후제1주적주정섭입량[(3.79±0.47)kg·d-1·g-1]명현고우CON-S조[(1.68±0.68)kg·d-1·g-1](P<0.05),이PS-S조동물재빙수응격후제3주적주정섭입명현소우CON-S조(P<0.05).령외,PS-S조자대해마CA2구화전액피질M1구적소백단백표체균명현저우CON-S조(15.82±0.30,26.52±0.12,P<0.05).결론 잉기응격가영향성년자성자대적주정섭취행위,차과정가능여해마화전액피질소백단백표체적감소유관.
Objective To observe the effects of prenatal stress on voluntary ethanol intake(EI) and brain parvalbumin (PV) expression in adult female rat offspring. Methods Six pregnant SD rats were randomly divided into prenatal stress (PS, n= 3) and control group (CON, n=3). During the late gestational periods, rats in PS were subjected to restraint stress for consecutive 3 days. The adult female offspring in PS were randomly divided into PS plus stress group(PS-S, n=6) and PS without stress group(PS-NS, n=6). The adult female offspring in CON were randomly divided into stress(CON-S, n = 6) and no stress group(CON-NS, n = 6). The rats in PS-S and CON-S were subjected to restraint stress, and 3 weeks later ice-water stress. All the rats in each group were of fered free choice between water and ethanol solution. Then EI of every rat was assessed every day. The PV expression in hippocampns and frontal cortex were detected by immunohistochemistry. Results The basal El of PS-NS at the 1st, 4th and 6th week were significantly less than that of CON-NS(P<0.05). The EI of PS-S at the 1st week after restraint stress was significantly higher than that of CON-S (P<0.05). But EI of PS-S at the 3rd week after ice-water stress was much less than that of CON-S (P<0.05). The PV expression of PS-S offspring in the hippocampal CA2 and frontal cortex M1 were obviously lower than that of CON-S(P<0.05). Conclusion Prenatal stress could affect on alcohol preference of adult female rat offspring, which possibly is related to the reduction of PV expression in brain.