中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2010年
1期
40-43
,共4页
王擒云%杨建平%张慧娟%戴体俊
王擒雲%楊建平%張慧娟%戴體俊
왕금운%양건평%장혜연%대체준
二异丙酚%延髓%受体,GABA-A%痛觉过敏
二異丙酚%延髓%受體,GABA-A%痛覺過敏
이이병분%연수%수체,GABA-A%통각과민
Propofol%Medulla oblongata%Receptors,CABA-A%Hyperalgesia
目的 探讨延髓头端腹内侧核γ-氨基丁酸A亚型(GABA_A)受体在异丙酚致大鼠痛觉过敏中的作用.方法 清洁级SD大鼠64只,雌雄不拘,月龄2～3月,体重250～300 g,随机分为4组(n=16):溶媒对照组(C组)、异丙酚组(P组)、荷包牡丹碱组(B组)及荷包牡丹碱+异丙酚组(BP组).参照脑立体定位图谱定位大鼠延髓头端腹内侧核,C组注射人工脑脊液(荷包牡丹碱溶媒)0.4 μl,5 min后注射二甲基亚砜(异丙酚溶媒)0.4μl,P组注射异丙酚0.4μl(4μg),B组注射荷包牡丹碱0.4μl(10 ng),BP组注射荷包牡丹碱0.4 μl(10 ng),5 min后注射异丙酚0.4 μl(4 μg),各药物均在30 s内注射完毕,30 s后拔针.各取8只大鼠分别进行热板实验和福尔马林实验,记录热痛阈、福尔马林疼痛评分及其第1时相和第2时相累计疼痛评分.结果 热板实验中,P组热痛阈低于C组,BP组给药后20 min时热痛阈明显高于P组(P<0.05或0.01),其余时点差异无统计学意义(P>0.05).福尔马林实验中,P组福尔马林疼痛评分高于C组,BP组各时点福尔马林疼痛评分和第1、2时相累计疼痛评分均明显低于P组(P<0.05或0.01).结论 延髓头端腹内侧核GABA_A受体部分介导了异丙酚致大鼠痛觉过敏作用.
目的 探討延髓頭耑腹內側覈γ-氨基丁痠A亞型(GABA_A)受體在異丙酚緻大鼠痛覺過敏中的作用.方法 清潔級SD大鼠64隻,雌雄不拘,月齡2～3月,體重250～300 g,隨機分為4組(n=16):溶媒對照組(C組)、異丙酚組(P組)、荷包牡丹堿組(B組)及荷包牡丹堿+異丙酚組(BP組).參照腦立體定位圖譜定位大鼠延髓頭耑腹內側覈,C組註射人工腦脊液(荷包牡丹堿溶媒)0.4 μl,5 min後註射二甲基亞砜(異丙酚溶媒)0.4μl,P組註射異丙酚0.4μl(4μg),B組註射荷包牡丹堿0.4μl(10 ng),BP組註射荷包牡丹堿0.4 μl(10 ng),5 min後註射異丙酚0.4 μl(4 μg),各藥物均在30 s內註射完畢,30 s後拔針.各取8隻大鼠分彆進行熱闆實驗和福爾馬林實驗,記錄熱痛閾、福爾馬林疼痛評分及其第1時相和第2時相纍計疼痛評分.結果 熱闆實驗中,P組熱痛閾低于C組,BP組給藥後20 min時熱痛閾明顯高于P組(P<0.05或0.01),其餘時點差異無統計學意義(P>0.05).福爾馬林實驗中,P組福爾馬林疼痛評分高于C組,BP組各時點福爾馬林疼痛評分和第1、2時相纍計疼痛評分均明顯低于P組(P<0.05或0.01).結論 延髓頭耑腹內側覈GABA_A受體部分介導瞭異丙酚緻大鼠痛覺過敏作用.
목적 탐토연수두단복내측핵γ-안기정산A아형(GABA_A)수체재이병분치대서통각과민중적작용.방법 청길급SD대서64지,자웅불구,월령2～3월,체중250～300 g,수궤분위4조(n=16):용매대조조(C조)、이병분조(P조)、하포모단감조(B조)급하포모단감+이병분조(BP조).삼조뇌입체정위도보정위대서연수두단복내측핵,C조주사인공뇌척액(하포모단감용매)0.4 μl,5 min후주사이갑기아풍(이병분용매)0.4μl,P조주사이병분0.4μl(4μg),B조주사하포모단감0.4μl(10 ng),BP조주사하포모단감0.4 μl(10 ng),5 min후주사이병분0.4 μl(4 μg),각약물균재30 s내주사완필,30 s후발침.각취8지대서분별진행열판실험화복이마림실험,기록열통역、복이마림동통평분급기제1시상화제2시상루계동통평분.결과 열판실험중,P조열통역저우C조,BP조급약후20 min시열통역명현고우P조(P<0.05혹0.01),기여시점차이무통계학의의(P>0.05).복이마림실험중,P조복이마림동통평분고우C조,BP조각시점복이마림동통평분화제1、2시상루계동통평분균명현저우P조(P<0.05혹0.01).결론 연수두단복내측핵GABA_A수체부분개도료이병분치대서통각과민작용.
Objective To investigate the effects of propofol injected into rostral ventromedial medulla (RVM) on nociceptive responses and examine whether GABA_A resceptor is involved in the mechanism. Methods Sixty-four pathogen free SD rats of both sexes aged 2-3 months weighing 250-300 g were randomly divided into 4 groups ( n = 16 each): group I control (group C) ; group Ⅱ propofol (group P) ; group Ⅲ bicuculline (group B) and group Ⅳ B + P (group BP) . The animals were anesthetized with intraperitoneal 1% pentobarbital 50 mg/kg. Their heads were fixed with stereotactic apparatus. An tracar was inserted into RVM for microinjection of propofol and/or bicuculline. The noxious responses were evaluated by hot plate test (response latency was measured) and formalin test (intraplantar injection of 2.0% formalin 100 μl) . Pain was scored (0 = no pain, 3 = severe pain) . Results Both hot plate test and formalin test showed that hyperalgesia was induced by microinjection of propofol into RVM. In hot plate test hyperalgesia induced by injection of propofol (4μg/0.4μl) into RVM was antagonized at 20 min after microinjection of bicuculline (10 ng/0.4μl) into RVM. In formalin test pain scores were significantly lower at 1, 5,10,20,30,40,50 and 60 min after intraplantar formalin injection in group BP than in group P.Conclusion GABA_A receptor in RVM partially mediates propofol-induced hyperalgesia.
