国际免疫学杂志
國際免疫學雜誌
국제면역학잡지
INTERNATIONAL JOURNAL OF IMMUNOLOGY
2009年
3期
173-175
,共3页
林乐勋%赵文然%佟雷%武帅钦%刘慧敏%刘惠彬%栾天%王博%马倩倩%钟照华
林樂勛%趙文然%佟雷%武帥欽%劉慧敏%劉惠彬%欒天%王博%馬倩倩%鐘照華
림악훈%조문연%동뢰%무수흠%류혜민%류혜빈%란천%왕박%마천천%종조화
人腺病毒%H9c2细胞%microRNA芯片%心肌炎
人腺病毒%H9c2細胞%microRNA芯片%心肌炎
인선병독%H9c2세포%microRNA심편%심기염
Human adenovirus%H9c2 cell%microRNA microarray%Myocarditis
目的 研究腺病毒感染对大鼠心肌来源的H9c2细胞microRNA表达谱的影响.方法 用人腺病毒3型(Ad3)攻击成层和未成层的H9c2细胞,6 h后收获总RNA,基因芯片检测microRNA表达谱.结果 比较未成层与成层的H9c2细胞microRNh的表达谱,H9c2细胞成层后有54种microRNA表达显著下调,7种microRNA表达显著上调.与未感染病毒的细胞比较,Ad3感染的未成层H9c2细胞有41种microRNA表达显著下调,12种microRNA表达显著上调.与未感染病毒的细胞比较,Ad3感染成层H9c2细胞后有8种microRNA的表达显著下调.62种microRNA的表达显著上调.结论 细胞不同的生长状态及Ad3感染显著改变了H9c2细胞的microRNA表达谱.
目的 研究腺病毒感染對大鼠心肌來源的H9c2細胞microRNA錶達譜的影響.方法 用人腺病毒3型(Ad3)攻擊成層和未成層的H9c2細胞,6 h後收穫總RNA,基因芯片檢測microRNA錶達譜.結果 比較未成層與成層的H9c2細胞microRNh的錶達譜,H9c2細胞成層後有54種microRNA錶達顯著下調,7種microRNA錶達顯著上調.與未感染病毒的細胞比較,Ad3感染的未成層H9c2細胞有41種microRNA錶達顯著下調,12種microRNA錶達顯著上調.與未感染病毒的細胞比較,Ad3感染成層H9c2細胞後有8種microRNA的錶達顯著下調.62種microRNA的錶達顯著上調.結論 細胞不同的生長狀態及Ad3感染顯著改變瞭H9c2細胞的microRNA錶達譜.
목적 연구선병독감염대대서심기래원적H9c2세포microRNA표체보적영향.방법 용인선병독3형(Ad3)공격성층화미성층적H9c2세포,6 h후수획총RNA,기인심편검측microRNA표체보.결과 비교미성층여성층적H9c2세포microRNh적표체보,H9c2세포성층후유54충microRNA표체현저하조,7충microRNA표체현저상조.여미감염병독적세포비교,Ad3감염적미성층H9c2세포유41충microRNA표체현저하조,12충microRNA표체현저상조.여미감염병독적세포비교,Ad3감염성층H9c2세포후유8충microRNA적표체현저하조.62충microRNA적표체현저상조.결론 세포불동적생장상태급Ad3감염현저개변료H9c2세포적microRNA표체보.
Objective To study the microRNA expression profile of rat myoeardium-derived H9c2 cells in-fected with human adenovirus type 3(Ad3).Methods Confluent and unconfluent H9c2 cells were attacked with Ad3.Six hour later total RNAs were harvested and microRNA expression profiling was analyzed by mi-cmRNA array.Results The expression of 54 microRNAs Was down-regulated and 7 micwRNAs Was up-reg-ulated in COnnuent H9c2 cells compared with that in unconiluent H9c2 cells.The expression of 41 miemR-NAs wag down-regulated and 12 Was up-regulated in unconfluent H9e2 cells infected with Ad3 compared with that in unconiluent H9c2 cells without Ad3 infection.There were 8 microRNAs down-regulated and 62 up-regulated in confluent H9c2 infected with Ad3 compared with that in comquent H9c2 ceils without Ad3 infec-tion.Condusion The microRNA expression profile,correlating with different cell growth states,was siguif-icanfly altered by human adenovirus infection.
