中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2008年
24期
1715-1718
,共4页
王永兰%费丽思%靳趁心%刘士有
王永蘭%費麗思%靳趁心%劉士有
왕영란%비려사%근진심%류사유
雌激素%牙周炎%牙槽骨质丢失%骨保护素
雌激素%牙週炎%牙槽骨質丟失%骨保護素
자격소%아주염%아조골질주실%골보호소
Estrogens%Periodontitis%Alveolar bone loss%Osteoprotegerin
目的 研究雌激素缺乏对牙周炎大鼠牙槽骨中骨保护素(OPG)表达的影响,探讨雌激素调节牙槽骨改建的机制.方法 采用切除双侧卵巢及牙周结扎的方法建立雌激素缺乏的大鼠牙周炎动物模型,观察大鼠牙周组织病理改变,应用免疫组织化学技术观察OPG在牙槽骨的表达,比较卵巢切除(OVX,n=18)、假手术(Sham,n=17)及雌二醇治疗(OVX+E2,n=18)3组大鼠牙槽骨OPG表达吸光度(A)值的差异.结果 各组大鼠组织学观察均显示牙槽骨骨小梁稀疏,牙槽骨表面可见破骨细胞及骨吸收陷窝,OVX组更为显著.Sham组、OVX组及OVX+E2组的牙槽骨OPG阳性表达A值分别为38 799±6228、27 854±5246和37 146±6394,OVX组吸光度低于Sham组和OVX+E2组(均P＜0.05),而Sham组和OVX+E2组之间差异无统计学意义(P＞0.05).结论 雌激素缺乏降低牙槽骨中骨保护素的表达,促进实验性牙周炎大鼠牙槽骨的吸收.
目的 研究雌激素缺乏對牙週炎大鼠牙槽骨中骨保護素(OPG)錶達的影響,探討雌激素調節牙槽骨改建的機製.方法 採用切除雙側卵巢及牙週結扎的方法建立雌激素缺乏的大鼠牙週炎動物模型,觀察大鼠牙週組織病理改變,應用免疫組織化學技術觀察OPG在牙槽骨的錶達,比較卵巢切除(OVX,n=18)、假手術(Sham,n=17)及雌二醇治療(OVX+E2,n=18)3組大鼠牙槽骨OPG錶達吸光度(A)值的差異.結果 各組大鼠組織學觀察均顯示牙槽骨骨小樑稀疏,牙槽骨錶麵可見破骨細胞及骨吸收陷窩,OVX組更為顯著.Sham組、OVX組及OVX+E2組的牙槽骨OPG暘性錶達A值分彆為38 799±6228、27 854±5246和37 146±6394,OVX組吸光度低于Sham組和OVX+E2組(均P＜0.05),而Sham組和OVX+E2組之間差異無統計學意義(P＞0.05).結論 雌激素缺乏降低牙槽骨中骨保護素的錶達,促進實驗性牙週炎大鼠牙槽骨的吸收.
목적 연구자격소결핍대아주염대서아조골중골보호소(OPG)표체적영향,탐토자격소조절아조골개건적궤제.방법 채용절제쌍측란소급아주결찰적방법건립자격소결핍적대서아주염동물모형,관찰대서아주조직병리개변,응용면역조직화학기술관찰OPG재아조골적표체,비교란소절제(OVX,n=18)、가수술(Sham,n=17)급자이순치료(OVX+E2,n=18)3조대서아조골OPG표체흡광도(A)치적차이.결과 각조대서조직학관찰균현시아조골골소량희소,아조골표면가견파골세포급골흡수함와,OVX조경위현저.Sham조、OVX조급OVX+E2조적아조골OPG양성표체A치분별위38 799±6228、27 854±5246화37 146±6394,OVX조흡광도저우Sham조화OVX+E2조(균P＜0.05),이Sham조화OVX+E2조지간차이무통계학의의(P＞0.05).결론 자격소결핍강저아조골중골보호소적표체,촉진실험성아주염대서아조골적흡수.
Objective To investigate the effects of estrogen-deficiency upon the expression of osteoprotegerin(OPG)in alveolar bone so as to clarify the role of estrogen in alveolaf bone metabolism．Methods Fifty-three SD rats were randomly divided into 3 groups:OVX group(n=18)undergoing bilateral ovariectomy,OVX+E2 group(n=18),undergoing bilateral ovariectomy and subcutaneous injection of estradiol once 3 days since day 2 after the operation,and sham operation group(n=17)undergoing sham operation.Four weeks after the operation periodontal ligature was conducted and hish-sugar diet was fed to establish periodontitis models.Four weeks later the rats were killed.The morphological changes and expression of OPG in alveolar bone were observed by light microscopy and immunohistochemistry.Results All the rats showed sparseness of bone trabeculae in alveolar bone and osteoclasts and lacuna at the surface of alveolar bone.Especially the rats of the group OVX.The A values(absorption of the OPG expression)in the alveolar bone of the OVX groups was 27 854±5246,significantly lower than those of the sham operation group and OVX+E,group(38 799±6228 and 37 146±6394 respectively,both P<0.05)without significant difference between the sham operation group and OVX+E2 group(P>0.05).Conclusion Estrogen deficiency decreases the expression of OPG in alveolar bone,and enhances the absorption of alveolar bone.
