中华肿瘤杂志
中華腫瘤雜誌
중화종류잡지
CHINESE JOURNAL OF ONCOLOGY
2009年
4期
260-264
,共5页
罗花南%李湘平%刘雄%张国华%刘友利
囉花南%李湘平%劉雄%張國華%劉友利
라화남%리상평%류웅%장국화%류우리
趋化因子受体4%间质细胞衍生因子1α%鼻咽肿瘤%器官特异性转移
趨化因子受體4%間質細胞衍生因子1α%鼻嚥腫瘤%器官特異性轉移
추화인자수체4%간질세포연생인자1α%비인종류%기관특이성전이
CXCR4%SDF-1α%Nasopharyngeal neoplasms%Organ-specific metastasis
目的 研究趋化因子受体4(CXCR4)在鼻咽癌细胞中的表达,间质细胞衍牛因子1α(SDF-1α)在鼻咽癌远处靶器官中的表达,探讨CXCR4和(或)SDF-1α在鼻咽癌器官特异性转移中的作用.方法 应用逆转录聚合酶链反应(RT-PCR)和免疫组织化学法分析30例鼻咽癌、15例正常鼻咽组织中CXCR4 mRNA和蛋白的表达及其同临床病理学因素之间的相关性,应用免疫组织化学法分析鼻咽癌患者的正常颈部淋巴结(包括颈深上和颈深下淋巴结)、骨髓、肺、肝脏和肾脏、结肠(各5例)中SDF-1α蛋白的表达.结果 RT-PCR检测结果显示,鼻咽癌组织中CXCR4 mRNA相对表达强度(0.71±0.22)显著高于正常鼻咽组织(0.14±0.07;F=27.94,P<0.05);免疫组织化学检测结果显示,鼻咽癌组织中CXCR4蛋白的表达(1.58±0.59)显著高于正常鼻咽组织(0.51±0.22;F=17.75,P<0.05).鼻咽癌组织中CXCR4 mRNA和蛋白的表达与临床分期、淋巴结转移、细胞分化程度显著相关(均P<0.05).SDF-1α蛋白在鼻咽癌患者的颈深上淋巴结、骨髓、肺、肝脏中表达较高(2.35±0.67),而在颈深下淋巴结、肾脏和结肠中表达较弱(0.68±0.23),差异有统计学意义(t=10.13,P<0.01).结论 CXCR4的表达与鼻咽癌的转移密切相关,CXCR4和(或)SDF-1α在鼻咽癌器官特异性转移中可能发挥重要作用.
目的 研究趨化因子受體4(CXCR4)在鼻嚥癌細胞中的錶達,間質細胞衍牛因子1α(SDF-1α)在鼻嚥癌遠處靶器官中的錶達,探討CXCR4和(或)SDF-1α在鼻嚥癌器官特異性轉移中的作用.方法 應用逆轉錄聚閤酶鏈反應(RT-PCR)和免疫組織化學法分析30例鼻嚥癌、15例正常鼻嚥組織中CXCR4 mRNA和蛋白的錶達及其同臨床病理學因素之間的相關性,應用免疫組織化學法分析鼻嚥癌患者的正常頸部淋巴結(包括頸深上和頸深下淋巴結)、骨髓、肺、肝髒和腎髒、結腸(各5例)中SDF-1α蛋白的錶達.結果 RT-PCR檢測結果顯示,鼻嚥癌組織中CXCR4 mRNA相對錶達彊度(0.71±0.22)顯著高于正常鼻嚥組織(0.14±0.07;F=27.94,P<0.05);免疫組織化學檢測結果顯示,鼻嚥癌組織中CXCR4蛋白的錶達(1.58±0.59)顯著高于正常鼻嚥組織(0.51±0.22;F=17.75,P<0.05).鼻嚥癌組織中CXCR4 mRNA和蛋白的錶達與臨床分期、淋巴結轉移、細胞分化程度顯著相關(均P<0.05).SDF-1α蛋白在鼻嚥癌患者的頸深上淋巴結、骨髓、肺、肝髒中錶達較高(2.35±0.67),而在頸深下淋巴結、腎髒和結腸中錶達較弱(0.68±0.23),差異有統計學意義(t=10.13,P<0.01).結論 CXCR4的錶達與鼻嚥癌的轉移密切相關,CXCR4和(或)SDF-1α在鼻嚥癌器官特異性轉移中可能髮揮重要作用.
목적 연구추화인자수체4(CXCR4)재비인암세포중적표체,간질세포연우인자1α(SDF-1α)재비인암원처파기관중적표체,탐토CXCR4화(혹)SDF-1α재비인암기관특이성전이중적작용.방법 응용역전록취합매련반응(RT-PCR)화면역조직화학법분석30례비인암、15례정상비인조직중CXCR4 mRNA화단백적표체급기동림상병이학인소지간적상관성,응용면역조직화학법분석비인암환자적정상경부림파결(포괄경심상화경심하림파결)、골수、폐、간장화신장、결장(각5례)중SDF-1α단백적표체.결과 RT-PCR검측결과현시,비인암조직중CXCR4 mRNA상대표체강도(0.71±0.22)현저고우정상비인조직(0.14±0.07;F=27.94,P<0.05);면역조직화학검측결과현시,비인암조직중CXCR4단백적표체(1.58±0.59)현저고우정상비인조직(0.51±0.22;F=17.75,P<0.05).비인암조직중CXCR4 mRNA화단백적표체여림상분기、림파결전이、세포분화정도현저상관(균P<0.05).SDF-1α단백재비인암환자적경심상림파결、골수、폐、간장중표체교고(2.35±0.67),이재경심하림파결、신장화결장중표체교약(0.68±0.23),차이유통계학의의(t=10.13,P<0.01).결론 CXCR4적표체여비인암적전이밀절상관,CXCR4화(혹)SDF-1α재비인암기관특이성전이중가능발휘중요작용.
Objective To explore the role of CXCR4/SDF-1α axis in organ-specific metastasis of nasopharyngeal carcinoma (NPC) by assessment of CXCR4 expression in NPC cells and SDF-1α expression in distant target organs of NPC. Methods Thirty patients with NPC and fifteen normal subjects were recruited in this study. The expressions of CXCR4 in NPC and normal cases were identified by RT-PCR and immunohistochemistry (IHC), then the relationship between CXCR4 expression and clinicopathological factors was analyzed. IHC was also used to analyze the SDF-1α protein expression in normal cervical lymph nodes (including normal superior and inferior deep cervical lymph nodes), bone marrow, lung, liver, kidney and colon tissues of NPC patients (5 cases/each group). Results The relative expression level of CXCR4 mRNA in NPC (0.71±0.22) was significantly higher than that of normal nasopharynx tissues (0.14±0.07, F=27.94, P<0.05). The relative expression level of CXCR4 protein in NPC (1.58±0.59) was significantly higher than that of normal nasopharynx tissues (0.51±0.22, F=17.75, P<0.05). The high expression levels of CXCR4 mRNA and protein in NPC were closely related to clinical stage, cervical lymph node metastasis and cancer cell differentiation (P<0.05). SDF-1α protein was strongly expressed in normal superior deep cervical lymph nodes, bone marrow, lung and liver (2.35±0.67), but absent or very poor expression in inferior deep cervical lymph nodes, kidney and colon tissues (0.68±0.23), and the differences between them were statistically significant (t=10.13, P<0.01). Conclusion CXCR4 is closely correlated to metastasis of nasopharyngeal carcinoma. CXCR4/SDF-1α axis may play an important role in organ-specific metastasis of NPC.
