CAJ | 학술논문

目的研究雄激素受体（AR）基因三核苷酸CAG微卫星多态性与前列腺癌（PC）分期和分级的关系，探讨其与肿瘤生物学特性的相关性。方法运用聚合酶链反应-单链构象多态性分析法（PCR-SSCP），对37例PC标本的AR基因CAG微卫星数量进行测定，并进行不同病变期别、分化级别间的比较及统计学分析。结果 AR基因CAG微卫星数量在病变B期与C～D期分别为25.04±1.88和22.14±2.64（P=0.002）；其短的AR基因CAG（数量＜22）的分布为：B期13.0%(3/23)，C～D期28.6%(4/14)；其长的AR基因CAG（数量≥22）的分布为：B期87.0%(20/23)，C～D期71.4%(10/14)，差异有显著性（P=0.029）。AR基因CAG微卫星数量随PC病变分化程度的增高而增加，低分化为23.64±2.56，中分化为24.29±3.45，高分化为25.6±1.67，在高分化与低分化间差异有显著性（P=0.047）。短组CAG微卫星的分布比例随病变分化程度的升高而降低，长组CAG微卫星的分布比例随病变分化程度的升高而增加。结论 AR基因CAG微卫星数量的多少可能是构成前列腺癌不同生物学特性的因素之一，短的CAG微卫星的AR基因可能与恶性度较高的前列腺癌表型有关。
목적연구웅격소수체（AR）기인삼핵감산CAG미위성다태성여전렬선암（PC）분기화분급적관계，탐토기여종류생물학특성적상관성。방법운용취합매련반응-단련구상다태성분석법（PCR-SSCP），대37례PC표본적AR기인CAG미위성수량진행측정，병진행불동병변기별、분화급별간적비교급통계학분석。결과 AR기인CAG미위성수량재병변B기여C～D기분별위25.04±1.88화22.14±2.64（P=0.002）；기단적AR기인CAG（수량＜22）적분포위：B기13.0%(3/23)，C～D기28.6%(4/14)；기장적AR기인CAG（수량≥22）적분포위：B기87.0%(20/23)，C～D기71.4%(10/14)，차이유현저성（P=0.029）。AR기인CAG미위성수량수PC병변분화정도적증고이증가，저분화위23.64±2.56，중분화위24.29±3.45，고분화위25.6±1.67，재고분화여저분화간차이유현저성（P=0.047）。단조CAG미위성적분포비례수병변분화정도적승고이강저，장조CAG미위성적분포비례수병변분화정도적승고이증가。결론 AR기인CAG미위성수량적다소가능시구성전렬선암불동생물학특성적인소지일，단적CAG미위성적AR기인가능여악성도교고적전렬선암표형유관。
Objective　To explore the relationship between the CAG mirosatellite in androgen receptor(AR)gene and the stage and grade of prostate cancer(PC).Methods　The number of CAG repeats in exon 1 of the AR gene was measured in 37 PC tissues by PCR-SSCP analysis, and its association with stages and grades of PC determined.Results　The average number of CAG repeats of AR gene differed significantly between PC in stage B (25.04±1.88) and in stage C～D (24.14±2.64)(P=0.002). The frequency of short CAG(＜22) was 13.0%(3/23) in stage B and 28.6%(4/14) in stage C～D, while that of long CAG(≥22) was 87.0%(20/23) and 71.4%(10/14) (P=0.029 ), respectively. The average number of CAG repeats increased with the decrease in tumor cell differentiation. The frequency of short CAG repeats dropped with the increase in tumor cell differentiation while the opposite was true for that of long CAG repeats.Conclusion　The number of CAG repeats in AR gene may be one of the factors determining the biologic characteratics PC. Short CAG repeats may signify aggressiveness of PC.