中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2008年
2期
101-103
,共3页
刘文培%张兵%谢志萍%钟礼立%瞿小旺%郑丽舒%高寒春%漆正宇%匡治州%张万菊%段招军%侯云德
劉文培%張兵%謝誌萍%鐘禮立%瞿小旺%鄭麗舒%高寒春%漆正宇%劻治州%張萬菊%段招軍%侯雲德
류문배%장병%사지평%종례립%구소왕%정려서%고한춘%칠정우%광치주%장만국%단초군%후운덕
呼吸道感染%变性肺病毒%病毒包膜蛋白质类%序列分析
呼吸道感染%變性肺病毒%病毒包膜蛋白質類%序列分析
호흡도감염%변성폐병독%병독포막단백질류%서렬분석
Respiratory tract infections%Metapneumovirus%Viral envelope proteins%Sequence analysis
目的 研究中国湖南地区人偏肺病毒(hMPV)的基因分型及其膜蛋白G序列的遗传学特征,比较分析其区域性流行特点.方法收集2005年中国湖南地区232份住院儿童鼻咽抽吸物(NPA)样本进行常见呼吸道病毒筛查,并扩增hMPV阳性样本的膜蛋白G全序列,与GenBank中已知hMPV参考株及其他地区公布的相关资料进行比对、进化等分子遗传学特征分析.结果从232份临床样本中共检测得到17份(7.3%)hMPV阳性样本,与其他病毒混合感染率达35%.扩增出其中13份hMPV样本的G蛋白全序列,分属于4种亚型(A1、A2、B1、B2).核酸长度类型有4种(711,675,660,696nt),N-连接糖基化位点数目和位置、半胱氨酸残基数目等特征与已报道的同期北京地区、日本、北美等地区hMPV调查分析结果不尽一致.结论中国湖南地区与其他地区同期hMPV调查分析结果各有特点,反映出hMPV变异显著,具有明显的地区流行特征.
目的 研究中國湖南地區人偏肺病毒(hMPV)的基因分型及其膜蛋白G序列的遺傳學特徵,比較分析其區域性流行特點.方法收集2005年中國湖南地區232份住院兒童鼻嚥抽吸物(NPA)樣本進行常見呼吸道病毒篩查,併擴增hMPV暘性樣本的膜蛋白G全序列,與GenBank中已知hMPV參攷株及其他地區公佈的相關資料進行比對、進化等分子遺傳學特徵分析.結果從232份臨床樣本中共檢測得到17份(7.3%)hMPV暘性樣本,與其他病毒混閤感染率達35%.擴增齣其中13份hMPV樣本的G蛋白全序列,分屬于4種亞型(A1、A2、B1、B2).覈痠長度類型有4種(711,675,660,696nt),N-連接糖基化位點數目和位置、半胱氨痠殘基數目等特徵與已報道的同期北京地區、日本、北美等地區hMPV調查分析結果不儘一緻.結論中國湖南地區與其他地區同期hMPV調查分析結果各有特點,反映齣hMPV變異顯著,具有明顯的地區流行特徵.
목적 연구중국호남지구인편폐병독(hMPV)적기인분형급기막단백G서렬적유전학특정,비교분석기구역성류행특점.방법수집2005년중국호남지구232빈주원인동비인추흡물(NPA)양본진행상견호흡도병독사사,병확증hMPV양성양본적막단백G전서렬,여GenBank중이지hMPV삼고주급기타지구공포적상관자료진행비대、진화등분자유전학특정분석.결과종232빈림상양본중공검측득도17빈(7.3%)hMPV양성양본,여기타병독혼합감염솔체35%.확증출기중13빈hMPV양본적G단백전서렬,분속우4충아형(A1、A2、B1、B2).핵산장도류형유4충(711,675,660,696nt),N-련접당기화위점수목화위치、반광안산잔기수목등특정여이보도적동기북경지구、일본、북미등지구hMPV조사분석결과불진일치.결론중국호남지구여기타지구동기hMPV조사분석결과각유특점,반영출hMPV변이현저,구유명현적지구류행특정.
Objective To understand the genotypes of human metapneumovirus (hMPV) and the genetic character of hMPV attachment protein G sequence in Hunan, China. Methods 232 nasopharyngeal aspirates (NPA) samples from hospitalized children with aeute respiratory infections were collected from Hunan, China in 2005. HMPV was detected. The full length of G glycoprotein genes were amplified and sequenced. Bioinformatics soft-wares were employed to analyze the sequences. Results 17/232 (7.3% ) were showed hMPV positive. And co-infection rate with other viruses is 35% .The diagnoses of these hMPV positive cases are pneumonia,bronchiolitis and bronchopneumonia. Phylogenetic analysis for G genes from 13 hMPVs revealed the existence of four major subgroups: A1, A2, B1, B2 in Hunan, China in 2005. There are four types of sequence lengths of hMPV G glycoprotein, which are 711, 675, 660, 696nt. It is different in potential N-linked glycosylation sites and number of eysteine residues among these hMPVs of Hunan, China and Beijing, China. Also it is different from those in Japan and North America. Conclusion The investigation of hMPV from Hunan, China in 2005 revealed the high speed of genetic variation and the marked character of geographic epidemic differences.
