CAJ | 학술논문

目的将PCR指纹图用于个体间HLA-DP的基因水平交叉配型，确定供-受体间DPB基因的相容性。方法引入猴的类DPB等位基因的扩增产物作通用性异源双链生成物（UHG），使原不能产生PCR指纹图的DPB基因扩增产物在非变性PAGE中显示特定的“卫星条带”，从而可用于供-受体间HLA-DP基因的相容性测定。结果 11株HLA纯合的B淋巴细胞系和随机个体进行PCR-DPB指纹图及交叉配型分析，能敏感地反映个体间DP基因的差异，特别是在纯合子DPB*0201和杂合子DPB*0402/0501中初步发现了可能存在着未能被PCR-RFLP区分的新的亚型或新的等位基因，但是尚不能区分DPB*0201和DPB*0402两个等位基因，有待今后进一步探索。结论初步表明该技术可有效判别供-受体DPB基因匹配性，并有简单、快速等优点。
목적장PCR지문도용우개체간HLA-DP적기인수평교차배형，학정공-수체간DPB기인적상용성。방법인입후적류DPB등위기인적확증산물작통용성이원쌍련생성물（UHG），사원불능산생PCR지문도적DPB기인확증산물재비변성PAGE중현시특정적“위성조대”，종이가용우공-수체간HLA-DP기인적상용성측정。결과 11주HLA순합적B림파세포계화수궤개체진행PCR-DPB지문도급교차배형분석，능민감지반영개체간DP기인적차이，특별시재순합자DPB*0201화잡합자DPB*0402/0501중초보발현료가능존재착미능피PCR-RFLP구분적신적아형혹신적등위기인，단시상불능구분DPB*0201화DPB*0402량개등위기인，유대금후진일보탐색。결론초보표명해기술가유효판별공-수체DPB기인필배성，병유간단、쾌속등우점。
Objective　To determinate HLA-DPB gene compatibility between donors and recipients by HLA-DPB gene crossmatching with PCR-fingerprinting. Methods　By introducing a PCR product of monkey′s DPB-like gene as a universal heteroduplex generator (UHG), each PCR product of HLA-DPB allele that does not generate heteroduplexes originally in the non-denatured PAGE can form some specific “satellite” bands which are used to determine the compatibility of HLA-DP allele by crossmatching with PCR-fingerprinting. Results　The study shows in HLA either 11 homozygous BLCLs or heterozygous individuals, the crossmatching analysis with PCR-fingerprinting can distinguish the HLA-DP differences between individuals sensitively and also find some challenging results primarily in the homozygous DPB*0201 BLCLs and heterozygous DPB*0402/0501 individuals. However the difference between DPB*0201 allele and DPB*0402 allele is still distinguished. Conclusion　PCR-fingerprinting is simple and rapid method in determining the HLA-DP compatibility between donors and recipients at genetic level.