CAJ | 학술논문

目的利用噬菌体随机15肽库确定SLE相关多肽。方法从系统性红斑狼疮（SLE）病人血清中纯化抗dsDNA多克隆抗体，以此为筛选配基，对噬菌体随机15肽库进行亲和筛选。结果经3轮筛选，每轮的投入/产出比逐轮升高，提示筛选具有良好的富集效果。第3轮筛选后选其中18个克隆进行结合试验，结果显示有6个仅与SLE病人血清反应而不与正常人血清反应，经DNA序列测定，发现其中5个克隆序列相同，通过该序列推出插入的外源短肽序列，合成并纯化该肽段。用此肽段分别与20例SLE病人及20例正常人血清反应，结果差异有显著性。结论该肽段可能是与SLE相关的抗原多肽。
목적이용서균체수궤15태고학정SLE상관다태。방법종계통성홍반랑창（SLE）병인혈청중순화항dsDNA다극륭항체，이차위사선배기，대서균체수궤15태고진행친화사선。결과 경3륜사선，매륜적투입/산출비축륜승고，제시사선구유량호적부집효과。제3륜사선후선기중18개극륭진행결합시험，결과현시유6개부여SLE병인혈청반응이불여정상인혈청반응，경DNA서렬측정，발현기중5개극륭서렬상동，통과해서렬추출삽입적외원단태서렬，합성병순화해태단。용차태단분별여20례SLE병인급20례정상인혈청반응，결과차이유현저성。결론해태단가능시여SLE상관적항원다태。
Objective　The study of SLE related peptide by phage-display random 15 amino acid peptide library. Methods　Anti-dsDNA antibody was purified from the serum of the SLE patients. The antibody was used as the ligand for biopanning of a phage-displayed random peptide library of 15 amino acid residues. Results　After three rounds of screening, the ratio of output to input was increased. It indicated the efficient enrichment. At the third round of screening, eighteen clones were selected for binding test with the sera of patients and of normal people. The results showed that six clones could react with the patients′ serum but not the serum of normal people. By DNA sequencing, it was confirmed that in five clones the fragments inserted had the identical sequence. The peptide was synthesized from the deduced sequence. There is significant difference between the reactions of patients′ and normal people′s serum with the synthetic peptide. Conclusion　The peptide was possibly associated with SLE antigens.