中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2009年
3期
219-222
,共4页
章沿锋%姚尚龙%张小洺%张德仁
章沿鋒%姚尚龍%張小洺%張德仁
장연봉%요상룡%장소명%장덕인
痛觉过敏%脊髓%星形细胞%Toll样受体3
痛覺過敏%脊髓%星形細胞%Toll樣受體3
통각과민%척수%성형세포%Toll양수체3
Hyperalgesia%Spinal cord%Astrecytes%Toll like receptor 3
目的 评价脊髓星形胶质细胞Toll样受体3(TLR3)与大鼠痛觉过敏形成的关系.方法 雄性SD大鼠,体重180-250 g,取鞘内置管成功的大鼠126只,随机分为3组(n=42),正常对照组(C组);生理盐水组(NS组)鞘内注射生理盐水0.5 ml/kg,1次,d.连续7 d;痛觉过敏组(H组)腹腔注射米诺环素40me/kg+鞘内注射Poly(I:C)0.5 mg/kg,1次,d,连续7 d.各组于鞘内给药前1d和鞘内给药后1、3、5、7、10、14、21、28 d时测定机械痛阈和热痛阈;各组于鞘内给药后7 d时处死6只大鼠,取L4,5脊髓节段,采用免疫组化法测定脊髓背角胶质纤维酸性蛋白(GFAP)表达;各组于鞘内给药前1d和鞘内给药后1、7,14、21、28 d时各处死6只大鼠,取L4,5脊髓节段,采用RT-PCR法测定TLR3 mRNA表达.结果 与C组和Ns组比较,H组机械痛阈降低,脊髓背角GFAP和TLR3 mRNA表达上调(P<0.05).结论 TLR3与其特异性配体结合后,激活脊髓背角星形胶质细胞,诱发大鼠痛觉过敏.
目的 評價脊髓星形膠質細胞Toll樣受體3(TLR3)與大鼠痛覺過敏形成的關繫.方法 雄性SD大鼠,體重180-250 g,取鞘內置管成功的大鼠126隻,隨機分為3組(n=42),正常對照組(C組);生理鹽水組(NS組)鞘內註射生理鹽水0.5 ml/kg,1次,d.連續7 d;痛覺過敏組(H組)腹腔註射米諾環素40me/kg+鞘內註射Poly(I:C)0.5 mg/kg,1次,d,連續7 d.各組于鞘內給藥前1d和鞘內給藥後1、3、5、7、10、14、21、28 d時測定機械痛閾和熱痛閾;各組于鞘內給藥後7 d時處死6隻大鼠,取L4,5脊髓節段,採用免疫組化法測定脊髓揹角膠質纖維痠性蛋白(GFAP)錶達;各組于鞘內給藥前1d和鞘內給藥後1、7,14、21、28 d時各處死6隻大鼠,取L4,5脊髓節段,採用RT-PCR法測定TLR3 mRNA錶達.結果 與C組和Ns組比較,H組機械痛閾降低,脊髓揹角GFAP和TLR3 mRNA錶達上調(P<0.05).結論 TLR3與其特異性配體結閤後,激活脊髓揹角星形膠質細胞,誘髮大鼠痛覺過敏.
목적 평개척수성형효질세포Toll양수체3(TLR3)여대서통각과민형성적관계.방법 웅성SD대서,체중180-250 g,취초내치관성공적대서126지,수궤분위3조(n=42),정상대조조(C조);생리염수조(NS조)초내주사생리염수0.5 ml/kg,1차,d.련속7 d;통각과민조(H조)복강주사미낙배소40me/kg+초내주사Poly(I:C)0.5 mg/kg,1차,d,련속7 d.각조우초내급약전1d화초내급약후1、3、5、7、10、14、21、28 d시측정궤계통역화열통역;각조우초내급약후7 d시처사6지대서,취L4,5척수절단,채용면역조화법측정척수배각효질섬유산성단백(GFAP)표체;각조우초내급약전1d화초내급약후1、7,14、21、28 d시각처사6지대서,취L4,5척수절단,채용RT-PCR법측정TLR3 mRNA표체.결과 여C조화Ns조비교,H조궤계통역강저,척수배각GFAP화TLR3 mRNA표체상조(P<0.05).결론 TLR3여기특이성배체결합후,격활척수배각성형효질세포,유발대서통각과민.
Objective To evaluate the relationship between toll-like receptor 3(TLR3)of spinal astrocytes and development of hyperalgesia induced by intrathecal Poly(I:C)in rats.Methods Male SD-rats weighing 180-250 g were anesthetized with intraperitoneal(IT)10%chloral hydrate 3-3.5 ml/mg.Intrathecnl catheter was placed at L6-S1 interspace with the tip positioned at lumbar enlargement.One hundred and twenty.six SD rats in which IT catheters were successfully placed were randomly divided into 3 groups(n=42 each):group Ⅰ normal control(C);group Ⅱ received normal saline 0.5ml/kg IT, once a day for 7 consecutive days(NS); group Ⅲ received minocycline 40 mg/kg IP+Poly(1:C)0.5 mg/kg IT once a day for 7 consecutive days(H).Paw withdrawal threshold to von Frey filament stimulation(PWT)and paw withdrawal latency to neciceptive thermal stimulation(PWL)were measured at 1 d before operation and 1,3,5,7.10,14.21,28 day after IT injection.Six animals were kiUed at 1d before operation and 1,7,14,21,28 d after IT injection respectively in each group and the lumbar segment of the spinal cord Wae removed for determination of the expression of TLR3-mRNA(by RT-PCR)and GFAP in the spinal donsal hom(by immuno-histochemistry)(only on day 7 after IT injection).Results PWT was significantly decreased and the expression of GFAP and TLR3 mRNA in the spinal dorsal horn were significandy up-regulated in group H as compared with group C and NS.Conclusion TLB3.Iigand binding can activate astrocytes in the spinal dorsal hom and then induce hyperalgesia in rats.
