CAJ | 학술논문

目的明确新生隐球菌群体感应现象,分离鉴定其群体效应分子.方法观察以不同密度接种的新生隐球菌TUP1△株生长情况,取高密度生长的培养滤过液加入低密度接种TUP1△株的培养基中,观察滤过液促其生长作用,提纯并分析滤过液中的活性物质.结果新生隐球菌TUP1△株具有密度依赖生长特性,只有高于特定密度接种才能形成正常菌落,从高密度生长菌落培养滤过液中分离出一种11肽,可促低密度TUP1△株生长,命名为quorum sensing peptide 1(QSP1).人工合成该活性物质QSP1,同样可使低密度接种的TUP1△菌株形成正常菌落.结论新生隐球菌存在群体感应现象,其群体效应分子为QSP1.
목적 명학신생은구균군체감응현상,분리감정기군체효응분자.방법 관찰이불동밀도접충적신생은구균TUP1△주생장정황,취고밀도생장적배양려과액가입저밀도접충TUP1△주적배양기중,관찰려과액촉기생장작용,제순병분석려과액중적활성물질.결과 신생은구균TUP1△주구유밀도의뢰생장특성,지유고우특정밀도접충재능형성정상균락,종고밀도생장균락배양려과액중분리출일충11태,가촉저밀도TUP1△주생장,명명위quorum sensing peptide 1(QSP1).인공합성해활성물질QSP1,동양가사저밀도접충적TUP1△균주형성정상균락.결론 신생은구균존재군체감응현상,기군체효응분자위QSP1.
Objective To clarify the quorum sensing and identify the quorum sensing molecular of Cryptococcus neoformans. Methods The growth of Cryptococcus neoformans TUP1 △ strain at different inoculum size was observed. The culture filtrate of high cell density TUP1△ strain was collected and plated on medium with low density TUP1△ cell, and the growth of low cell density TUP1△ was recorded. Finally, the active molecule in culture filtrate was purified and identified.Results The growth of Cryptococcus neoformans TUP1△ strain was density dependent and the colony could be normally formed only above a specific density. The filtrate from a high cell density TUP1△culture could promote the growth of low cell density TUP1△, and the active molecule in this culture filtrate was identified to be an oligopeptide composed of 11 amino acids pepride, named quorum sensing peptide 1(QSP1). QSP1 was artificially synthesized, which could also promote the colony forming of TUP1△ strain inoculated at low cell density. Conclusion Quorum sensing exists in Cryptococcus neoformans, and the quorum sensing molecular is QSP1.