白血病·淋巴瘤
白血病·淋巴瘤
백혈병·림파류
JOURNAL OF LEUKEMIA & LYMPHOMA
2011年
4期
229-231
,共3页
姚军萍%汪兴洪%黄东平%苏贵平
姚軍萍%汪興洪%黃東平%囌貴平
요군평%왕흥홍%황동평%소귀평
多发性骨髓瘤%RNA%缺氧诱导因子1%实时荧光定量逆转录聚合酶链反应
多髮性骨髓瘤%RNA%缺氧誘導因子1%實時熒光定量逆轉錄聚閤酶鏈反應
다발성골수류%RNA%결양유도인자1%실시형광정량역전록취합매련반응
Multiple myeloma%RNA%Hypoxia-inducible factor 1%Real-time fluorescence quantitative reverse transcriptase polymerase chain reaction
目的 探讨多发性骨髓瘤(MM)患者缺氧诱导因子1 α(HIF-1 α)基因mRNA表达和临床各指标的相关性,了解HIF-1 α基因在MM患者中的表达和意义.方法 应用实时荧光定量聚合酶链反应(RQ-PCR)28例检测MM患者和22例骨科外伤患者或非血液系统恶性肿瘤患者(对照组)骨髓单个核细胞中的HIF-1 α mRNA,以β-actin作为内参照,用SDS分析软件测定Ct值,用2-△△Ct表示目的 基因的量.结果 HIF-1 α mRNA在MM骨髓单个核细胞中表达为对照组的12.68倍,HIF-1 α mRNA水平(以-△Ct表示)与患者血清β2-微球蛋白(r=0.575,P=0.000)、红细胞沉降率(r=0.522,P=0.000)、乳酸脱氢酶(r=0.286,P=0.044)、C反应蛋白(r=0.356,P=0.011)水平呈正相关,与患者血红蛋白(Hb)水平呈负相关(r=-0.556,P=0.000).结论 HIF-1 α mRNA在MM组织中高表达,与临床多项指标有关,可作为临床监测指标之一,有可能成为肿瘤分子靶向治疗中新的靶点.
目的 探討多髮性骨髓瘤(MM)患者缺氧誘導因子1 α(HIF-1 α)基因mRNA錶達和臨床各指標的相關性,瞭解HIF-1 α基因在MM患者中的錶達和意義.方法 應用實時熒光定量聚閤酶鏈反應(RQ-PCR)28例檢測MM患者和22例骨科外傷患者或非血液繫統噁性腫瘤患者(對照組)骨髓單箇覈細胞中的HIF-1 α mRNA,以β-actin作為內參照,用SDS分析軟件測定Ct值,用2-△△Ct錶示目的 基因的量.結果 HIF-1 α mRNA在MM骨髓單箇覈細胞中錶達為對照組的12.68倍,HIF-1 α mRNA水平(以-△Ct錶示)與患者血清β2-微毬蛋白(r=0.575,P=0.000)、紅細胞沉降率(r=0.522,P=0.000)、乳痠脫氫酶(r=0.286,P=0.044)、C反應蛋白(r=0.356,P=0.011)水平呈正相關,與患者血紅蛋白(Hb)水平呈負相關(r=-0.556,P=0.000).結論 HIF-1 α mRNA在MM組織中高錶達,與臨床多項指標有關,可作為臨床鑑測指標之一,有可能成為腫瘤分子靶嚮治療中新的靶點.
목적 탐토다발성골수류(MM)환자결양유도인자1 α(HIF-1 α)기인mRNA표체화림상각지표적상관성,료해HIF-1 α기인재MM환자중적표체화의의.방법 응용실시형광정량취합매련반응(RQ-PCR)28례검측MM환자화22례골과외상환자혹비혈액계통악성종류환자(대조조)골수단개핵세포중적HIF-1 α mRNA,이β-actin작위내삼조,용SDS분석연건측정Ct치,용2-△△Ct표시목적 기인적량.결과 HIF-1 α mRNA재MM골수단개핵세포중표체위대조조적12.68배,HIF-1 α mRNA수평(이-△Ct표시)여환자혈청β2-미구단백(r=0.575,P=0.000)、홍세포침강솔(r=0.522,P=0.000)、유산탈경매(r=0.286,P=0.044)、C반응단백(r=0.356,P=0.011)수평정정상관,여환자혈홍단백(Hb)수평정부상관(r=-0.556,P=0.000).결론 HIF-1 α mRNA재MM조직중고표체,여림상다항지표유관,가작위림상감측지표지일,유가능성위종류분자파향치료중신적파점.
Objective To analyze the correlation between the expression of HIF-1α in multiple myeloma (MM) and clinical indexes in order to illustrate the expression and implication of HIF-1α gene in MM. Methods RQ-PCR method was used to amplify HIF-1α mRNA of bone marrow mononuclear cells isolated from 28 cases of MM patients and the control group. β-actin was used as internal standard. HIF-1α mRNA expression was analyzed by SDS software and the ratios of HIF-1α/ β-actin were calculated. Results The relative expression level of HIF-1α mRNA in MM bone marrow mononuclear cells was 12.68 times as that in control group. HIF-1α mRNA was positively correlated with β2-MG (r =0.575, P =0.000), ESR (r =0.522,P =0.000), LDH (r=0.286, P=0.044) and CRP (r =0.356, P =0.011). There was a negative correlation between HIF-1α mRNA and Hb (r =-0.556, P =0.000). Conclusion The expression of HIF-1α mRNA was up-regulated and HIF-1α was related to a number of clinical indexes. HIF-1α may be used to estimate the progress of MM and hopeful to be a new molecular target in cancer therapy.
